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Protein

3-keto-L-gulonate-6-phosphate decarboxylase UlaD

Gene

ulaD

Organism
Escherichia coli (strain 55989 / EAEC)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the decarboxylation of 3-keto-L-gulonate-6-P into L-xylulose-5-P. Is involved in the anaerobic L-ascorbate utilization.UniRule annotation

Catalytic activityi

3-dehydro-L-gulonate 6-phosphate = L-xylulose 5-phosphate + CO2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111SubstrateUniRule annotation
Metal bindingi33 – 331MagnesiumUniRule annotation
Metal bindingi62 – 621MagnesiumUniRule annotation
Sitei64 – 641Transition state stabilizerUniRule annotation
Sitei67 – 671Transition state stabilizerUniRule annotation
Binding sitei192 – 1921SubstrateUniRule annotation

GO - Molecular functioni

  1. 3-dehydro-L-gulonate-6-phosphate decarboxylase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. orotidine-5'-phosphate decarboxylase activity Source: InterPro

GO - Biological processi

  1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
  2. L-ascorbic acid catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciECOL585055:GJOM-4826-MONOMER.
UniPathwayiUPA00263; UER00378.

Names & Taxonomyi

Protein namesi
Recommended name:
3-keto-L-gulonate-6-phosphate decarboxylase UlaDUniRule annotation (EC:4.1.1.85UniRule annotation)
Alternative name(s):
3-dehydro-L-gulonate-6-phosphate decarboxylaseUniRule annotation
KGPDCUniRule annotation
L-ascorbate utilization protein DUniRule annotation
Gene namesi
Name:ulaDUniRule annotation
Ordered Locus Names:EC55989_4753
OrganismiEscherichia coli (strain 55989 / EAEC)
Taxonomic identifieri585055 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000746: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2162163-keto-L-gulonate-6-phosphate decarboxylase UlaDPRO_1000165141Add
BLAST

Expressioni

Inductioni

Induced by L-ascorbate. Repressed by UlaR.UniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi585055.EC55989_4753.

Structurei

3D structure databases

ProteinModelPortaliB7LCQ5.
SMRiB7LCQ5. Positions 2-216.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HPS/KGPDC family. KGPDC subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0269.
HOGENOMiHOG000226068.
KOiK03078.
OMAiTIPTMKA.
OrthoDBiEOG66B435.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01267. UlaD.
InterProiIPR023942. 3-keto-L-gulonate6Pdecase_UlaD.
IPR013785. Aldolase_TIM.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.

Sequencei

Sequence statusi: Complete.

B7LCQ5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLPMLQVAL DNQTMDSAYE TTRLIAEEVD IIEVGTILCV GEGVRAVRDL
60 70 80 90 100
KALYPHKIVL ADAKIADAGK ILSRMCFEAN ADWVTVICCA DINTAKGALD
110 120 130 140 150
VAKEFNGDVQ IELTGYWTWE QAQQWRDAGI QQVVYHRSRD AQAAGVAWGE
160 170 180 190 200
ADITAIKRLS DMGFKVTVTG GLALEDLPLF KGIPIHVFIA GRSIRDAASP
210
VEAARQFKRS IAELWG
Length:216
Mass (Da):23,649
Last modified:February 10, 2009 - v1
Checksum:iCDF287AC1D1BAD68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928145 Genomic DNA. Translation: CAV01690.1.
RefSeqiYP_002405613.1. NC_011748.1.

Genome annotation databases

EnsemblBacteriaiCAV01690; CAV01690; EC55989_4753.
GeneIDi7147915.
KEGGieck:EC55989_4753.
PATRICi38483098. VBIEscCol113220_4776.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928145 Genomic DNA. Translation: CAV01690.1.
RefSeqiYP_002405613.1. NC_011748.1.

3D structure databases

ProteinModelPortaliB7LCQ5.
SMRiB7LCQ5. Positions 2-216.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi585055.EC55989_4753.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAV01690; CAV01690; EC55989_4753.
GeneIDi7147915.
KEGGieck:EC55989_4753.
PATRICi38483098. VBIEscCol113220_4776.

Phylogenomic databases

eggNOGiCOG0269.
HOGENOMiHOG000226068.
KOiK03078.
OMAiTIPTMKA.
OrthoDBiEOG66B435.

Enzyme and pathway databases

UniPathwayiUPA00263; UER00378.
BioCyciECOL585055:GJOM-4826-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01267. UlaD.
InterProiIPR023942. 3-keto-L-gulonate6Pdecase_UlaD.
IPR013785. Aldolase_TIM.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 55989 / EAEC.

Entry informationi

Entry nameiULAD_ECO55
AccessioniPrimary (citable) accession number: B7LCQ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: January 7, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.