B7LAS9 (MENH_ECO55) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Short name=SHCHC synthase
EC=4.2.99.20

Gene names

Name:	menH
Ordered Locus Names:	EC55989_2511

Organism

Escherichia coli (strain 55989 / EAEC) [Complete proteome] [HAMAP]

Taxonomic identifier

585055 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	252 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro By similarity. HAMAP-Rule MF_01660
Catalytic activity	5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate. HAMAP-Rule MF_01660
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 3/8. HAMAP-Rule MF_01660
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. MenH family.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	menaquinone biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 252	252	2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase HAMAP-Rule MF_01660		PRO_1000187105

Sequences

	Sequence		Length	Mass (Da)
	B7LAS9 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 066D7E861099DAE1	FASTA	252	27,697
	10 20 30 40 50 60 MILHAQAKHG KPGLPWLVFL HGFSGDCHEW QEVGEAFADY SRLYVDLPGH GGSAAISVDG 70 80 90 100 110 120 FDDVTDLLRK TLVSYNILDF WLVGYSLGGR VAMMAACQGL AGLCGVIVEG GHPGLQNAEQ 130 140 150 160 170 180 RAERQRSDRQ WAQRFRTEPL TAVFADWYQQ PVFASLNDDQ RRELVALRSN NNGATLAAML 190 200 210 220 230 240 EATSLAVQPD LRANLSARTF AFYYLCGERD SKFRALAAEL AADCHVIPRA GHNAHRENPA 250 GVIASLAQIL RF

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 55989 / EAEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928145 Genomic DNA. Translation: CAU98379.1.
RefSeq	YP_002403533.1. NC_011748.1.
3D structure databases
ProteinModelPortal	B7LAS9.
SMR	B7LAS9. Positions 3-251.
ModBase	Search...
Protein-protein interaction databases
STRING	585055.EC55989_2511.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAU98379; CAU98379; EC55989_2511.
GeneID	7146818.
KEGG	eck:EC55989_2511.
PATRIC	38478517. VBIEscCol113220_2551.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0596.
HOGENOM	HOG000028072.
KO	K08680.
OMA	LGGRIAM.
ProtClustDB	PRK11126.
Enzyme and pathway databases
BioCyc	ECOL585055:GJOM-2562-MONOMER.
UniPathway	UPA00079; UER00900.
Family and domain databases
HAMAP	MF_01660. MenH.
InterPro	IPR022485. SHCHC_synthase_MenH. [Graphical view]
TIGRFAMs	TIGR03695. menH_SHCHC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MENH_ECO55

Accession

Primary (citable) accession number: B7LAS9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program