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B7LAS9 (MENH_ECO55) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Short name=SHCHC synthase
EC=4.2.99.20
Gene names
Name:menH
Ordered Locus Names:EC55989_2511
OrganismEscherichia coli (strain 55989 / EAEC) [Complete proteome] [HAMAP]
Taxonomic identifier585055 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro By similarity. HAMAP-Rule MF_01660

Catalytic activity

5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate. HAMAP-Rule MF_01660

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 3/8. HAMAP-Rule MF_01660

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01660

Sequence similarities

Belongs to the AB hydrolase superfamily. MenH family.

Ontologies

Keywords
   Biological processMenaquinone biosynthesis
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmenaquinone biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2522522-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase HAMAP-Rule MF_01660
PRO_1000187105

Sequences

Sequence LengthMass (Da)Tools
B7LAS9 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 066D7E861099DAE1

FASTA25227,697
        10         20         30         40         50         60 
MILHAQAKHG KPGLPWLVFL HGFSGDCHEW QEVGEAFADY SRLYVDLPGH GGSAAISVDG 

        70         80         90        100        110        120 
FDDVTDLLRK TLVSYNILDF WLVGYSLGGR VAMMAACQGL AGLCGVIVEG GHPGLQNAEQ 

       130        140        150        160        170        180 
RAERQRSDRQ WAQRFRTEPL TAVFADWYQQ PVFASLNDDQ RRELVALRSN NNGATLAAML 

       190        200        210        220        230        240 
EATSLAVQPD LRANLSARTF AFYYLCGERD SKFRALAAEL AADCHVIPRA GHNAHRENPA 

       250 
GVIASLAQIL RF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU928145 Genomic DNA. Translation: CAU98379.1.
RefSeqYP_002403533.1. NC_011748.1.

3D structure databases

ProteinModelPortalB7LAS9.
SMRB7LAS9. Positions 3-251.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING585055.EC55989_2511.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAU98379; CAU98379; EC55989_2511.
GeneID7146818.
KEGGeck:EC55989_2511.
PATRIC38478517. VBIEscCol113220_2551.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0596.
HOGENOMHOG000028072.
KOK08680.
OMALGGRIAM.
OrthoDBEOG689HPM.

Enzyme and pathway databases

BioCycECOL585055:GJOM-2562-MONOMER.
UniPathwayUPA00079; UER00900.

Family and domain databases

Gene3D3.40.50.1820. 1 hit.
HAMAPMF_01660. MenH.
InterProIPR029058. AB_hydrolase.
IPR022485. SHCHC_synthase_MenH.
[Graphical view]
SUPFAMSSF53474. SSF53474. 1 hit.
TIGRFAMsTIGR03695. menH_SHCHC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMENH_ECO55
AccessionPrimary (citable) accession number: B7LAS9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: June 11, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways