B7LAF8 (GAL1_ECO55) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactokinase
EC=2.7.1.6

Alternative name(s):
Galactose kinase

Gene names

Name:	galK
Ordered Locus Names:	EC55989_0736

Organism

Escherichia coli (strain 55989 / EAEC) [Complete proteome] [HAMAP]

Taxonomic identifier

585055 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of the gamma-phosphate of ATP to D-galactose to form alpha-D-galactose-1-phosphate (Gal-1-P) By similarity. HAMAP-Rule MF_00246
Catalytic activity	ATP + D-galactose = ADP + alpha-D-galactose 1-phosphate. HAMAP-Rule MF_00246
Pathway	Carbohydrate metabolism; galactose metabolism. HAMAP-Rule MF_00246
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00246.
Sequence similarities	Belongs to the GHMP kinase family. GalK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Galactose metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	galactose metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP galactokinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Galactokinase HAMAP-Rule MF_00246		PRO_1000125378
Regions
	Nucleotide binding	124 – 130	7	ATP By similarity
	Region	34 – 37	4	Substrate binding By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Metal binding	130	1	Magnesium By similarity
	Metal binding	162	1	Magnesium By similarity
	Binding site	223	1	Substrate By similarity
	Site	28	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7LAF8 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 7D886CE6FC48F0A1	FASTA	382	41,442
	10 20 30 40 50 60 MSLKEKTQSL FANAFGYPAT HTIQAPGRVN LIGEHTDYND GFVLPCAIDY QTVISCAPRD 70 80 90 100 110 120 DRKVRVMAAD YENQLDEFSL DAPIVAHENY QWANYVRGVV KHLQLRNNSF GGVDMVISGN 130 140 150 160 170 180 VPQGAGLSSS ASLEVAVGTV LQQLYHLPLD GAQIALNGQE AENQFVGCNC GIMDQLISAL 190 200 210 220 230 240 GKKDHALLID CRSLGTKAVS MPKGVAVVII NSNFKRTLVG SEYNTRREQC ETGARFFQQP 250 260 270 280 290 300 ALRDVTIEEF NAVAHELDPI VAKRVRHILT ENARTVEAAS ALEQGDLKRM GELMAESHAS 310 320 330 340 350 360 MRDDFEITVP QIDTLVEIVK AVIGDKGGVR MTGGGFGGCI VALIPEELVP AVQQAVAEQY 370 380 EAKTGIKETF YVCKPSQGAG QC

References

[1]

"Organised genome dynamics in the Escherichia coli species results in highly diverse adaptive paths." Touchon M., Hoede C., Tenaillon O., Barbe V., Baeriswyl S., Bidet P., Bingen E., Bonacorsi S., Bouchier C., Bouvet O., Calteau A., Chiapello H., Clermont O., Cruveiller S., Danchin A., Diard M., Dossat C., Karoui M.E. , Frapy E., Garry L., Ghigo J.M., Gilles A.M., Johnson J., Le Bouguenec C., Lescat M., Mangenot S., Martinez-Jehanne V., Matic I., Nassif X., Oztas S., Petit M.A., Pichon C., Rouy Z., Ruf C.S., Schneider D., Tourret J., Vacherie B., Vallenet D., Medigue C., Rocha E.P.C., Denamur E. PLoS Genet. 5:E1000344-E1000344(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 55989 / EAEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU928145 Genomic DNA. Translation: CAU96604.1.
RefSeq	YP_002401841.1. NC_011748.1.
3D structure databases
ProteinModelPortal	B7LAF8.
SMR	B7LAF8. Positions 11-379.
ModBase	Search...
Protein-protein interaction databases
STRING	585055.EC55989_0736.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAU96604; CAU96604; EC55989_0736.
GeneID	7146250.
KEGG	eck:EC55989_0736.
PATRIC	38474864. VBIEscCol113220_0750.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0153.
HOGENOM	HOG000241100.
KO	K00849.
OMA	MPCAINY.
ProtClustDB	PRK05101.
Enzyme and pathway databases
BioCyc	ECOL585055:GJOM-756-MONOMER.
UniPathway	UPA00214.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00246. Galactokinase.
InterPro	IPR000705. Galactokinase. IPR022963. Galactokinase_bac. IPR019741. Galactokinase_CS. IPR019539. GalKase_gal-bd. IPR013750. GHMP_kinase_C_dom. IPR006204. GHMP_kinase_N_dom. IPR006203. GHMP_knse_ATP-bd_CS. IPR006206. Mevalonate/galactokinase. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Pfam	PF10509. GalKase_gal_bdg. 1 hit. PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000530. Galactokinase. 1 hit.
PRINTS	PR00473. GALCTOKINASE. PR00959. MEVGALKINASE.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00131. gal_kin. 1 hit.
PROSITE	PS00106. GALACTOKINASE. 1 hit. PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GAL1_ECO55

Accession

Primary (citable) accession number: B7LAF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 24, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program