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B7L503 (MHPA_ECO55) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase

Short name=3-HCI hydroxylase
Short name=3-HPP hydroxylase
EC=1.14.13.127
Gene names
Name:mhpA
Ordered Locus Names:EC55989_0354
OrganismEscherichia coli (strain 55989 / EAEC) [Complete proteome] [HAMAP]
Taxonomic identifier585055 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length554 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI) By similarity. HAMAP-Rule MF_01652

Catalytic activity

3-(3-hydroxyphenyl)propanoate + NADH + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+. HAMAP-Rule MF_01652

(2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+. HAMAP-Rule MF_01652

Cofactor

FAD By similarity. HAMAP-Rule MF_01652

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP-Rule MF_01652

Sequence similarities

Belongs to the PheA/TfdB FAD monooxygenase family.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandFAD
Flavoprotein
NAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_process3-(3-hydroxy)phenylpropionate catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

3-phenylpropionate catabolic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_function3-(3-hydroxyphenyl)propionate hydroxylase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5545543-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase HAMAP-Rule MF_01652
PRO_1000186989

Regions

Nucleotide binding17 – 4630FAD Potential
Nucleotide binding285 – 29511FAD Potential

Sequences

Sequence LengthMass (Da)Tools
B7L503 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 1CFDCB632F3F3A24

FASTA55462,154
        10         20         30         40         50         60 
MAIQHPDIQP AVNHSVQVAI AGAGPVGLMM ANYLGQMGID VLVVEKLDKL IDYPRAIGID 

        70         80         90        100        110        120 
DEALRTMQSV GLVDDVLPHT TPWHAMRFLT PKGRCFADIQ PMTDEFGWPR RNAFIQPQVD 

       130        140        150        160        170        180 
AVMLEGVSRF PNVRCLFSRE LEAFSQQDDE VTLHLKTAEG QREIVKAQWL VACDGGASFV 

       190        200        210        220        230        240 
RRTLNVPFEG KTAPNQWIVV DIANDPLSTP HIYLCCDPVR PYVSAALPHA VRRFEFMVMP 

       250        260        270        280        290        300 
GETEEQLREP QNMRKLLSKV LPNPDNVELI RQRVYTHNAR LAQRFRIDRV LLAGDAAHIM 

       310        320        330        340        350        360 
PVWQGQGYNS GMRDAFNLAW KLALVIQGKA RDALLDTYQQ ERRDHAKAMI DLSVTAGNVL 

       370        380        390        400        410        420 
APPKRWQGTL RDGVSWLLNY LPPVKRYFLE MRFKPMPQYY GGALVREGEA KHSPVGKMFI 

       430        440        450        460        470        480 
QPKVTLENGD VTLLDNAIGA NFAVIGWGCN PLWGMSDEQI QQWRALGTRF IQVVPEVQIH 

       490        500        510        520        530        540 
TAQDNHDGVL RVGDTQGRLR SWFAQHNASL VVMRPDRFVA ATAIPQTLGK TLNKLASVMT 

       550 
LTRPDADVSV EKVA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CU928145 Genomic DNA. Translation: CAU96231.1.
RefSeqYP_002401478.1. NC_011748.1.

3D structure databases

ProteinModelPortalB7L503.
SMRB7L503. Positions 15-543.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING585055.EC55989_0354.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAU96231; CAU96231; EC55989_0354.
GeneID7146850.
KEGGeck:EC55989_0354.
PATRIC38474058. VBIEscCol113220_0362.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0654.
HOGENOMHOG000221401.
KOK05712.
OMARNRISAW.
OrthoDBEOG63RGN4.

Enzyme and pathway databases

BioCycECOL585055:GJOM-361-MONOMER.
UniPathwayUPA00714.

Family and domain databases

HAMAPMF_01652. MhpA.
InterProIPR023786. 3-HPP/3HCI_hydroxylase.
IPR002938. mOase_FAD-bd.
IPR003042. Rng_hydrolase-like.
[Graphical view]
PfamPF01494. FAD_binding_3. 1 hit.
[Graphical view]
PRINTSPR00420. RNGMNOXGNASE.
ProtoNetSearch...

Entry information

Entry nameMHPA_ECO55
AccessionPrimary (citable) accession number: B7L503
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways