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Protein

3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylase

Gene

mhpA

Organism
Escherichia coli (strain 55989 / EAEC)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the insertion of one atom of molecular oxygen into position 2 of the phenyl ring of 3-(3-hydroxyphenyl)propionate (3-HPP) and hydroxycinnamic acid (3HCI).UniRule annotation

Catalytic activityi

3-(3-hydroxyphenyl)propanoate + NADH + O2 = 3-(2,3-dihydroxyphenyl)propanoate + H2O + NAD+.UniRule annotation
(2E)-3-(3-hydroxyphenyl)prop-2-enoate + NADH + O2 = (2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + H2O + NAD+.UniRule annotation

Cofactori

FADUniRule annotation

Pathway:i3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 4630FADUniRule annotationAdd
BLAST
Nucleotide bindingi285 – 29511FADUniRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciECOL585055:GJOM-361-MONOMER.
UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylaseUniRule annotation (EC:1.14.13.127UniRule annotation)
Short name:
3-HCI hydroxylaseUniRule annotation
Short name:
3-HPP hydroxylaseUniRule annotation
Gene namesi
Name:mhpAUniRule annotation
Ordered Locus Names:EC55989_0354
OrganismiEscherichia coli (strain 55989 / EAEC)
Taxonomic identifieri585055 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000746 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5545543-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid hydroxylasePRO_1000186989Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliB7L503.
SMRiB7L503. Positions 15-543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PheA/TfdB FAD monooxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0654.
HOGENOMiHOG000221401.
OMAiNDPLGTP.
OrthoDBiEOG63RGN4.

Family and domain databases

HAMAPiMF_01652. MhpA.
InterProiIPR023786. 3-HPP/3HCI_hydroxylase.
IPR002938. mOase_FAD-bd.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7L503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIQHPDIQP AVNHSVQVAI AGAGPVGLMM ANYLGQMGID VLVVEKLDKL
60 70 80 90 100
IDYPRAIGID DEALRTMQSV GLVDDVLPHT TPWHAMRFLT PKGRCFADIQ
110 120 130 140 150
PMTDEFGWPR RNAFIQPQVD AVMLEGVSRF PNVRCLFSRE LEAFSQQDDE
160 170 180 190 200
VTLHLKTAEG QREIVKAQWL VACDGGASFV RRTLNVPFEG KTAPNQWIVV
210 220 230 240 250
DIANDPLSTP HIYLCCDPVR PYVSAALPHA VRRFEFMVMP GETEEQLREP
260 270 280 290 300
QNMRKLLSKV LPNPDNVELI RQRVYTHNAR LAQRFRIDRV LLAGDAAHIM
310 320 330 340 350
PVWQGQGYNS GMRDAFNLAW KLALVIQGKA RDALLDTYQQ ERRDHAKAMI
360 370 380 390 400
DLSVTAGNVL APPKRWQGTL RDGVSWLLNY LPPVKRYFLE MRFKPMPQYY
410 420 430 440 450
GGALVREGEA KHSPVGKMFI QPKVTLENGD VTLLDNAIGA NFAVIGWGCN
460 470 480 490 500
PLWGMSDEQI QQWRALGTRF IQVVPEVQIH TAQDNHDGVL RVGDTQGRLR
510 520 530 540 550
SWFAQHNASL VVMRPDRFVA ATAIPQTLGK TLNKLASVMT LTRPDADVSV

EKVA
Length:554
Mass (Da):62,154
Last modified:February 10, 2009 - v1
Checksum:i1CFDCB632F3F3A24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928145 Genomic DNA. Translation: CAU96231.1.

Genome annotation databases

EnsemblBacteriaiCAU96231; CAU96231; EC55989_0354.
PATRICi38474058. VBIEscCol113220_0362.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU928145 Genomic DNA. Translation: CAU96231.1.

3D structure databases

ProteinModelPortaliB7L503.
SMRiB7L503. Positions 15-543.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAU96231; CAU96231; EC55989_0354.
PATRICi38474058. VBIEscCol113220_0362.

Phylogenomic databases

eggNOGiCOG0654.
HOGENOMiHOG000221401.
OMAiNDPLGTP.
OrthoDBiEOG63RGN4.

Enzyme and pathway databases

UniPathwayiUPA00714.
BioCyciECOL585055:GJOM-361-MONOMER.

Family and domain databases

HAMAPiMF_01652. MhpA.
InterProiIPR023786. 3-HPP/3HCI_hydroxylase.
IPR002938. mOase_FAD-bd.
[Graphical view]
PfamiPF01494. FAD_binding_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 55989 / EAEC.

Entry informationi

Entry nameiMHPA_ECO55
AccessioniPrimary (citable) accession number: B7L503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: July 22, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.