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Protein

Sulfate adenylyltransferase subunit 1

Gene

cysN

Organism
Methylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

May be the GTPase, regulating ATP sulfurylase activity.UniRule annotation

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC), Adenylyl-sulfate kinase (cysC)
  3. Adenylylsulfate reductase, thioredoxin dependent (Mchl_2510)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 38GTPUniRule annotation8
Nucleotide bindingi110 – 114GTPUniRule annotation5
Nucleotide bindingi165 – 168GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMEXT440085:G1GUD-2521-MONOMER
UniPathwayiUPA00140; UER00204

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase large subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysNUniRule annotation
Ordered Locus Names:Mchl_2508
OrganismiMethylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
Taxonomic identifieri440085 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000002385 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001179161 – 469Sulfate adenylyltransferase subunit 1Add BLAST469

Proteomic databases

PRIDEiB7L0X9

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7L0X9
SMRiB7L0X9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 237tr-type GAdd BLAST216

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 38G1By similarity8
Regioni89 – 93G2By similarity5
Regioni110 – 113G3By similarity4
Regioni165 – 168G4By similarity4
Regioni202 – 204G5By similarity3

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229289
KOiK00956
OMAiFDPYEQN
OrthoDBiPOG091H06C6

Family and domain databases

HAMAPiMF_00062 Sulf_adenylyltr_sub1, 1 hit
InterProiView protein in InterPro
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR011779 SO4_adenylTrfase_lsu
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR02034 CysN, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

B7L0X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIHQSPEAF GYDAFLRQHQ NKEVLRFITC GSVDDGKSTL IGRLLHDTKQ
60 70 80 90 100
IFDDQVTALQ RDSRKHGTQG GEVDFALLVD GLQAEREQGI TIDVAYRFFS
110 120 130 140 150
TDRRSFIVAD TPGHEQYTRN MATGASTADL AVILVDARHG LTRQSRRHAL
160 170 180 190 200
LVSLLGIRRV ALAINKMDLV GWSQDKFEAI VSGFQAFAAP LNFTEVRAIP
210 220 230 240 250
LSAKNGDNVV LPGTAATWYT DVPLLRYLEE VPVKSEERAA AFRMPVQWVN
260 270 280 290 300
RPNSDFRGFS GLIASGSVAP GDAVTVAPSG KTSTIARIFT ADGDLERASE
310 320 330 340 350
GQSVTLVLAD EVDASRGAVI ATSDAPLTLT DSLDVRLFWA AESDLVPGAN
360 370 380 390 400
LWAKVGTQTV NAVVKAVHRR IDPETGQAGP ADKLAVNDIG DVTLTLDRQI
410 420 430 440 450
AVDPYAENRD TGSLILIDRE TTDTAALGLV QTIVASSKVA PAPTASVTAS
460
AEPARSGGLL AGLKRLFGG
Length:469
Mass (Da):50,231
Last modified:February 10, 2009 - v1
Checksum:i20B28B73863BD23F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001298 Genomic DNA Translation: ACK83350.1
RefSeqiWP_015950909.1, NC_011757.1

Genome annotation databases

EnsemblBacteriaiACK83350; ACK83350; Mchl_2508
KEGGimch:Mchl_2508

Similar proteinsi

Entry informationi

Entry nameiCYSN_METC4
AccessioniPrimary (citable) accession number: B7L0X9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: May 23, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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