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Protein

Sulfate adenylyltransferase subunit 1

Gene

cysN

Organism
Methylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May be the GTPase, regulating ATP sulfurylase activity.UniRule annotation

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC), Adenylyl-sulfate kinase (cysC)
  3. Adenylylsulfate reductase, thioredoxin dependent (Mchl_2510)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 388GTPUniRule annotation
Nucleotide bindingi110 – 1145GTPUniRule annotation
Nucleotide bindingi165 – 1684GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMCHL440085:GCXT-2559-MONOMER.
UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase large subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysNUniRule annotation
Ordered Locus Names:Mchl_2508
OrganismiMethylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
Taxonomic identifieri440085 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000002385 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Sulfate adenylyltransferase subunit 1PRO_1000117916Add
BLAST

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7L0X9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 237216tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni31 – 388G1By similarity
Regioni89 – 935G2By similarity
Regioni110 – 1134G3By similarity
Regioni165 – 1684G4By similarity
Regioni202 – 2043G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229289.
KOiK00956.
OMAiIQDVYKF.
OrthoDBiEOG6GR38Q.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7L0X9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIHQSPEAF GYDAFLRQHQ NKEVLRFITC GSVDDGKSTL IGRLLHDTKQ
60 70 80 90 100
IFDDQVTALQ RDSRKHGTQG GEVDFALLVD GLQAEREQGI TIDVAYRFFS
110 120 130 140 150
TDRRSFIVAD TPGHEQYTRN MATGASTADL AVILVDARHG LTRQSRRHAL
160 170 180 190 200
LVSLLGIRRV ALAINKMDLV GWSQDKFEAI VSGFQAFAAP LNFTEVRAIP
210 220 230 240 250
LSAKNGDNVV LPGTAATWYT DVPLLRYLEE VPVKSEERAA AFRMPVQWVN
260 270 280 290 300
RPNSDFRGFS GLIASGSVAP GDAVTVAPSG KTSTIARIFT ADGDLERASE
310 320 330 340 350
GQSVTLVLAD EVDASRGAVI ATSDAPLTLT DSLDVRLFWA AESDLVPGAN
360 370 380 390 400
LWAKVGTQTV NAVVKAVHRR IDPETGQAGP ADKLAVNDIG DVTLTLDRQI
410 420 430 440 450
AVDPYAENRD TGSLILIDRE TTDTAALGLV QTIVASSKVA PAPTASVTAS
460
AEPARSGGLL AGLKRLFGG
Length:469
Mass (Da):50,231
Last modified:February 10, 2009 - v1
Checksum:i20B28B73863BD23F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001298 Genomic DNA. Translation: ACK83350.1.
RefSeqiWP_015950909.1. NC_011757.1.

Genome annotation databases

EnsemblBacteriaiACK83350; ACK83350; Mchl_2508.
KEGGimch:Mchl_2508.
PATRICi22499255. VBIMetChl132133_2460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001298 Genomic DNA. Translation: ACK83350.1.
RefSeqiWP_015950909.1. NC_011757.1.

3D structure databases

ProteinModelPortaliB7L0X9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK83350; ACK83350; Mchl_2508.
KEGGimch:Mchl_2508.
PATRICi22499255. VBIMetChl132133_2460.

Phylogenomic databases

HOGENOMiHOG000229289.
KOiK00956.
OMAiIQDVYKF.
OrthoDBiEOG6GR38Q.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.
BioCyciMCHL440085:GCXT-2559-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium chloromethanicum CM4."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Marx C., Richardson P.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CM4 / NCIMB 13688.

Entry informationi

Entry nameiCYSN_METC4
AccessioniPrimary (citable) accession number: B7L0X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: December 9, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.