B7L0A5 (FTHS_METC4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	Mchl_0447

Organism

Methylobacterium chloromethanicum (strain CM4 / NCIMB 13688) [Complete proteome] [HAMAP]

Taxonomic identifier

440085 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 557	557	Formate--tetrahydrofolate ligase HAMAP MF_01543		PRO_1000185257
Regions
	Nucleotide binding	65 – 72	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7L0A5 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 969EF0DC2DE42C8B	FASTA	557	59,478
	10 20 30 40 50 60 MPSDIEIARA ATLKPIAQVA EKLGIPDEAL HNYGKHIAKI DHDFIASLEG KPEGKLVLVT 70 80 90 100 110 120 AISPTPAGEG KTTTTVGLGD ALNRIGKRAV MCLREPSLGP CFGMKGGAAG GGKAQVVPME 130 140 150 160 170 180 QINLHFTGDF HAITSAHSLA AALIDNHIYW ANELNIDVRR IHWRRVVDMN DRALRAINQS 190 200 210 220 230 240 LGGVANGFPR EDGFDITVAS EVMAVFCLAK NLADLEERLG RIVIAETRDR KPVTLADVKA 250 260 270 280 290 300 TGAMTVLLKD ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRTGLR LADYTVTEAG 310 320 330 340 350 360 FGADLGAEKF IDIKCRQTGL KPSSVVIVAT IRALKMHGGV NKKDLQAENL DALEKGFANL 370 380 390 400 410 420 ERHVNNVRSF GLPVVVGVNH FFQDTDAEHA RLKELCRDRL QVEAITCKHW AEGGAGAEAL 430 440 450 460 470 480 AQAVVKLAEG EQKPLTFAYE TETKITDKIK AIATKLYGAA DIQIESKAAT KLAGFEKDGY 490 500 510 520 530 540 GKLPVCMAKT QYSFSTDPTL MGAPSGHLVS VRDVRLSAGA GFVVVICGEI MTMPGLPKVP 550 AADTIRLDAN GQIDGLF

References

[1]

"Complete sequence of chromosome of Methylobacterium chloromethanicum CM4." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Marx C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CM4 / NCIMB 13688.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001298 Genomic DNA. Translation: ACK81383.1.
RefSeq	YP_002419311.1. NC_011757.1.
3D structure databases
ProteinModelPortal	B7L0A5.
SMR	B7L0A5. Positions 4-555.
ModBase	Search...
Protein-protein interaction databases
STRING	B7L0A5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7116455.
GenomeReviews	Gene locus Mchl_0447 in contig CP001298_GR.
KEGG	mch:Mchl_0447.
PATRIC	22495059. VBIMetChl132133_0407.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG677721.
OMA	TDFTVTI.
ProtClustDB	CLSK2330235.
Family and domain databases
HAMAP	MF_01543. FTHFS. [Tree]
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
KO	K01938.
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_METC4

Accession

Primary (citable) accession number: B7L0A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program