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B7L0A5 (FTHS_METC4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Formate--tetrahydrofolate ligase

EC=6.3.4.3
Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS
Gene names
Name:fhs
Ordered Locus Names:Mchl_0447
OrganismMethylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum) [Complete proteome] [HAMAP]
Taxonomic identifier440085 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543

Sequence similarities

Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
   Biological processOne-carbon metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfolic acid-containing compound biosynthetic process

Inferred from electronic annotation. Source: InterPro

tetrahydrofolate interconversion

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

formate-tetrahydrofolate ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 557557Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543
PRO_1000185257

Regions

Nucleotide binding65 – 728ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7L0A5 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 969EF0DC2DE42C8B

FASTA55759,478
        10         20         30         40         50         60 
MPSDIEIARA ATLKPIAQVA EKLGIPDEAL HNYGKHIAKI DHDFIASLEG KPEGKLVLVT 

        70         80         90        100        110        120 
AISPTPAGEG KTTTTVGLGD ALNRIGKRAV MCLREPSLGP CFGMKGGAAG GGKAQVVPME 

       130        140        150        160        170        180 
QINLHFTGDF HAITSAHSLA AALIDNHIYW ANELNIDVRR IHWRRVVDMN DRALRAINQS 

       190        200        210        220        230        240 
LGGVANGFPR EDGFDITVAS EVMAVFCLAK NLADLEERLG RIVIAETRDR KPVTLADVKA 

       250        260        270        280        290        300 
TGAMTVLLKD ALQPNLVQTL EGNPALIHGG PFANIAHGCN SVIATRTGLR LADYTVTEAG 

       310        320        330        340        350        360 
FGADLGAEKF IDIKCRQTGL KPSSVVIVAT IRALKMHGGV NKKDLQAENL DALEKGFANL 

       370        380        390        400        410        420 
ERHVNNVRSF GLPVVVGVNH FFQDTDAEHA RLKELCRDRL QVEAITCKHW AEGGAGAEAL 

       430        440        450        460        470        480 
AQAVVKLAEG EQKPLTFAYE TETKITDKIK AIATKLYGAA DIQIESKAAT KLAGFEKDGY 

       490        500        510        520        530        540 
GKLPVCMAKT QYSFSTDPTL MGAPSGHLVS VRDVRLSAGA GFVVVICGEI MTMPGLPKVP 

       550 
AADTIRLDAN GQIDGLF 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium chloromethanicum CM4."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Marx C., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CM4 / NCIMB 13688.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001298 Genomic DNA. Translation: ACK81383.1.
RefSeqYP_002419311.1. NC_011757.1.

3D structure databases

ProteinModelPortalB7L0A5.
SMRB7L0A5. Positions 4-555.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING440085.Mchl_0447.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK81383; ACK81383; Mchl_0447.
GeneID7116455.
KEGGmch:Mchl_0447.
PATRIC22495059. VBIMetChl132133_0407.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2759.
HOGENOMHOG000040280.
KOK01938.
OMAIANSVSM.
OrthoDBEOG6PCPSP.

Enzyme and pathway databases

BioCycMCHL440085:GCXT-455-MONOMER.
UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_01543. FTHFS.
InterProIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFTHS_METC4
AccessionPrimary (citable) accession number: B7L0A5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways