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B7KYJ1

- PDXA_METC4

UniProt

B7KYJ1 - PDXA_METC4

Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Methylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei141 – 1411SubstrateUniRule annotation
    Binding sitei142 – 1421SubstrateUniRule annotation
    Metal bindingi176 – 1761Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi221 – 2211Divalent metal cation; shared with dimeric partnerUniRule annotation
    Metal bindingi276 – 2761Divalent metal cation; shared with dimeric partnerUniRule annotation
    Binding sitei284 – 2841SubstrateUniRule annotation
    Binding sitei293 – 2931SubstrateUniRule annotation
    Binding sitei302 – 3021SubstrateUniRule annotation

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. cobalt ion binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

    Enzyme and pathway databases

    BioCyciMCHL440085:GCXT-3994-MONOMER.
    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
    Gene namesi
    Name:pdxAUniRule annotation
    Ordered Locus Names:Mchl_3930
    OrganismiMethylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
    Taxonomic identifieri440085 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
    ProteomesiUP000002385: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3493494-hydroxythreonine-4-phosphate dehydrogenasePRO_1000146490Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi440085.Mchl_3930.

    Structurei

    3D structure databases

    ProteinModelPortaliB7KYJ1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1995.
    HOGENOMiHOG000221592.
    KOiK00097.
    OMAiAIGTEDE.
    OrthoDBiEOG6GN6ZC.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B7KYJ1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASDDRPLAL TLGDPSGIGP EIALAAWQLR SERGVPPFQL IGDPEFLEAT    50
    AYRLGLSVPV AEVEPDDACE VFPRALPVLP LPSGAKVIAT PGAPDSANAG 100
    AIVESITAAV DLVRSGAASA VVTNPIAKFV LTRVGFAHPG HTEFLAALAA 150
    REGREPSLPV MMIWSDTLAV VPVTIHVALR RVPELLTQDL VERTARIVHA 200
    DLRARFGLEH PRLVLSGLNP HAGESGTMGT EDRDVLAPAV AVLRSEGIDI 250
    RGPLPADTLF HERARATYDV ALTPTHDQAL IPVKTLAFDE GVNVTLGLPF 300
    VRTSPDHGTA FDIAGKGLAK PDSLIAALRL AHRLAHRPAD TVLPFPARA 349
    Length:349
    Mass (Da):36,842
    Last modified:February 10, 2009 - v1
    Checksum:i5C41DBDAFDF48A06
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001298 Genomic DNA. Translation: ACK84742.1.
    RefSeqiYP_002422670.1. NC_011757.1.

    Genome annotation databases

    EnsemblBacteriaiACK84742; ACK84742; Mchl_3930.
    GeneIDi7117128.
    KEGGimch:Mchl_3930.
    PATRICi22502156. VBIMetChl132133_3898.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001298 Genomic DNA. Translation: ACK84742.1 .
    RefSeqi YP_002422670.1. NC_011757.1.

    3D structure databases

    ProteinModelPortali B7KYJ1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 440085.Mchl_3930.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK84742 ; ACK84742 ; Mchl_3930 .
    GeneIDi 7117128.
    KEGGi mch:Mchl_3930.
    PATRICi 22502156. VBIMetChl132133_3898.

    Phylogenomic databases

    eggNOGi COG1995.
    HOGENOMi HOG000221592.
    KOi K00097.
    OMAi AIGTEDE.
    OrthoDBi EOG6GN6ZC.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .
    BioCyci MCHL440085:GCXT-3994-MONOMER.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00557. pdxA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of chromosome of Methylobacterium chloromethanicum CM4."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Marx C., Richardson P.
      Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CM4 / NCIMB 13688.

    Entry informationi

    Entry nameiPDXA_METC4
    AccessioniPrimary (citable) accession number: B7KYJ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.UniRule annotation

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3