B7KVP7 (B7KVP7_METC4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2 HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022

Alternative name(s):
Glutamyl-tRNA synthetase 2 HAMAP MF_00022

Gene names

Name:	gltX2 HAMAP MF_00022
Ordered Locus Names:	Mchl_5220

Organism

Methylobacterium chloromethanicum (strain CM4 / NCIMB 13688) [Complete proteome] [HAMAP] EMBL ACK85982.1

Taxonomic identifier

440085 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00022
Cellular component	Cytoplasm HAMAP MF_00022
Ligand	ATP-binding HAMAP MF_00022 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00022 EMBL ACK85982.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	11 – 21	11	"HIGH" region By similarity HAMAP MF_00022
	Motif	242 – 246	5	"KMSKS" region By similarity HAMAP MF_00022
Sites
	Binding site	245	1	ATP By similarity HAMAP MF_00022

Sequences

	Sequence		Length	Mass (Da)
	B7KVP7 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 99297159AF588085	FASTA	449	48,854
	10 20 30 40 50 60 MAASPLVRFA PSPTGFLHIG NARPALLNAL FARREGGRFL LRLDDTDRER STEEFATAAA 70 80 90 100 110 120 EDLGWLGIKP DLFFRQSDRT ALYDTAAERL KAAGRLYPCY ETPEELDRRR KRQLGRGLPP 130 140 150 160 170 180 IYDRAALALT EAERAAFEAE GRKPHWRFKL DHRVVAWNDL VRGESHVDCA SLSDPVLVRA 190 200 210 220 230 240 DGSYLYTLPS VVDDAEVGVT HVIRGEDHVT NTGVQVQLFE ALAASVPAFG HHNLLTTADG 250 260 270 280 290 300 EGLSKRLGHL SLRSLREAGY EPAAVRSLAV LTGSAEAVRP IASLDELADL VDLAHLSRAP 310 320 330 340 350 360 ARFDPAELDG MNARLVHDMP LEDVRERLSA LGVPAEAAEA FWLAVRANLG RVSDAAAWWQ 370 380 390 400 410 420 VVNGPVTPVI AEPDFIARAA DLLPEAPFGP GTWKAWTDAV KAETGAKGRA LFMPLRLALT 430 440 GLDHGPDLAA LLPLIGRERA VRRLAGETA

References

[1]

"Complete sequence of chromosome of Methylobacterium chloromethanicum CM4." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Marx C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CM4 / NCIMB 13688.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001298 Genomic DNA. Translation: ACK85982.1.
RefSeq	YP_002423910.1. NC_011757.1.
3D structure databases
ProteinModelPortal	B7KVP7.
ModBase	Search...
Protein-protein interaction databases
STRING	B7KVP7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7113828.
GenomeReviews	Gene locus Mchl_5220 in contig CP001298_GR.
KEGG	mch:Mchl_5220.
PATRIC	22504754. VBIMetChl132133_5185.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	DQERIEW.
ProtClustDB	PRK12558.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B7KVP7_METC4

Accession

Primary (citable) accession number: B7KVP7

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)