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B7KSC6 (DDL_METC4) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Mchl_3173
OrganismMethylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum) [Complete proteome] [HAMAP]
Taxonomic identifier440085 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000189740

Regions

Domain101 – 301201ATP-grasp
Nucleotide binding127 – 18256ATP By similarity

Sites

Metal binding2511Magnesium or manganese 1 By similarity
Metal binding2681Magnesium or manganese 1 By similarity
Metal binding2681Magnesium or manganese 2 By similarity
Metal binding2701Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B7KSC6 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 304F7314D45BB63D

FASTA30732,744
        10         20         30         40         50         60 
MSKHVAVLMG GWSSEREISL RSGNACAAAL EGEGYRVTRV DVGPDIATVL TELRPDAALN 

        70         80         90        100        110        120 
ALHGPAGEDG TIQGLLEILK IPYTHSGVLA SALAMHKERA KTVMRAAGVS VPEGRVVNRH 

       130        140        150        160        170        180 
DAAKSHPLTP PYVVKPIAEG SSMGVIIVRD ERSHPPQILA SDEWVYGEEV LAETYVAGRE 

       190        200        210        220        230        240 
LTCAVLGDRA LGVTEIKPVS GEWYDFDAKY AGGGSIHVLP ADLKLNIYQR VQELALTAHQ 

       250        260        270        280        290        300 
ALGCRGVSRA DLRYDDTPGG TGALVVLEVN TQPGMTQTSL VPEIAAHAGQ SFGELVRWMV 


EDASLNR 

« Hide

References

[1]"Complete sequence of chromosome of Methylobacterium chloromethanicum CM4."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Marx C., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CM4 / NCIMB 13688.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001298 Genomic DNA. Translation: ACK84011.1.
RefSeqYP_002421939.1. NC_011757.1.

3D structure databases

ProteinModelPortalB7KSC6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING440085.Mchl_3173.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK84011; ACK84011; Mchl_3173.
GeneID7114172.
KEGGmch:Mchl_3173.
PATRIC22500644. VBIMetChl132133_3147.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycMCHL440085:GCXT-3232-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_METC4
AccessionPrimary (citable) accession number: B7KSC6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways