B7KPQ3 (B7KPQ3_METC4) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B HAMAP MF_01898
EC=5.99.1.3 HAMAP MF_01898

Gene names

Name:	gyrB HAMAP MF_01898
Ordered Locus Names:	Mchl_2800

Organism

Methylobacterium chloromethanicum (strain CM4 / NCIMB 13688) [Complete proteome] [HAMAP] EMBL ACK83639.1

Taxonomic identifier

440085 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Methylobacteriaceae › Methylobacterium

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. SAAS SAAS002288 HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01898
Subcellular location	Cytoplasm By similarity HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family. HAMAP MF_01898

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_01898
Ligand	ATP-binding SAAS SAAS002288 HAMAP MF_01898 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS002288 HAMAP MF_01898
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B7KPQ3 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 289D8D376E53EADD	FASTA	815	89,567
	10 20 30 40 50 60 MADSPNTEHA DSYGAESIRV LKGLDAVRKR PGMYIGDTDD GSGLHHMIYE VVDNAIDEAL 70 80 90 100 110 120 AGHADLVTVT LNADGSVTVS DNGRGIPTDI HREEGVSAAE VIMTQLHAGG KFDQNSYKVS 130 140 150 160 170 180 GGLHGVGVSV VNALSTSLKL RIWRAGKEHR MEFRHGDAVA PLEVVGPAGD RRGTEVTFLP 190 200 210 220 230 240 STETFTMTEF DYPTLEKRLR ELAFLNSGVR IVVTDARHAE HKREELCYEG GVEAFVRYLD 250 260 270 280 290 300 RSRKPVDGMT KPVTVLGERD GIRVEVALWW NDSFNETVLP FTNNIPQRDG GTHMAGFRAA 310 320 330 340 350 360 LTRQLTGYAE SSGIAKREKV SLTGDDCREG LTAVISVQVP DPKFSSQTKD KLVSSEVRPA 370 380 390 400 410 420 VENILNEGLS TWLEENPAQA KSVMGKVVLA AAAREAARKA RETITRKGAL DIASLPGKLA 430 440 450 460 470 480 DCQERDPSKC ELLLVEGDSA GGSAKQGRDR TFQAVLPLRG KILNVERVRA DRMLSSAEIG 490 500 510 520 530 540 TLITALGAGI GRSSTDREGF NPDKLRYHRI IIMTDADVDG SHIRTLLLTF FFRQMPELIE 550 560 570 580 590 600 RGHLYIAQPP LYKAERGRRV LYLKDERALE DYLIDQGVEG AVLRLASGTE FGGAQLKTLV 610 620 630 640 650 660 EEARAFRGIL HGLHTRYDRS VVEQSVLAGA FAKDVAENLG EAEVLADRTA KRLDRIADEI 670 680 690 700 710 720 EKGWTGAASE GGYVFSRTLR SVTQVANLDA TLLASQEARR LAERAETLRE IYEEPVTLAR 730 740 750 760 770 780 KGDETELYGP VGLFEAVMAF GRKGLQLQRY KGLGEMTAQQ LWETTLDRDV RSLLQVKVKD 790 800 810 TTDADDLFVK LMGDVVEPRR EFIQENALSV ANLDV

References

[1]

"Complete sequence of chromosome of Methylobacterium chloromethanicum CM4." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Marx C., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CM4 / NCIMB 13688.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001298 Genomic DNA. Translation: ACK83639.1.
RefSeq	YP_002421567.1. NC_011757.1.
3D structure databases
ProteinModelPortal	B7KPQ3.
SMR	B7KPQ3. Positions 18-388.
ModBase	Search...
Protein-protein interaction databases
STRING	B7KPQ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7115029.
GenomeReviews	Gene locus Mchl_2800 in contig CP001298_GR.
KEGG	mch:Mchl_2800.
PATRIC	22499873. VBIMetChl132133_2766.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG304889.
OMA	DCSSRDP.
ProtClustDB	PRK14939.
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 2 hits.
KO	K02470.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01059. GyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B7KPQ3_METC4

Accession

Primary (citable) accession number: B7KPQ3

Entry history

Integrated into UniProtKB/TrEMBL:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)