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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Methylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei310Coenzyme AUniRule annotation1
Binding sitei499ATPUniRule annotation1
Binding sitei514ATPUniRule annotation1
Binding sitei522Coenzyme A; via carbonyl oxygenUniRule annotation1
Binding sitei525ATPUniRule annotation1
Metal bindingi536Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi538Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi541Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei583Coenzyme AUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi386 – 388ATPUniRule annotation3
Nucleotide bindingi410 – 415ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMEXT440085:G1GUD-2803-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Mchl_2785
OrganismiMethylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
Taxonomic identifieri440085 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
Proteomesi
  • UP000002385 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001640501 – 652Acetyl-coenzyme A synthetaseAdd BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei608N6-acetyllysineUniRule annotation1

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiB7KPN8

Structurei

3D structure databases

ProteinModelPortaliB7KPN8
SMRiB7KPN8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni190 – 193Coenzyme A bindingUniRule annotation4

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229981
KOiK01895
OMAiDHWWHDL
OrthoDBiPOG091H059D

Family and domain databases

CDDicd05966 ACS, 1 hit
HAMAPiMF_01123 Ac_CoA_synth, 1 hit
InterProiView protein in InterPro
IPR011904 Ac_CoA_lig
IPR032387 ACAS_N
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF16177 ACAS_N, 1 hit
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
TIGRFAMsiTIGR02188 Ac_CoA_lig_AcsA, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

B7KPN8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKVIDVQD AWRDRALIDE AKYKEMYEAS VSDPETFWGE HGKRIDWSTP
60 70 80 90 100
FSKVKNTSFA PGDVSIKWFE DGRTNVALNC IDRHLATRGD QTAIIWEGDD
110 120 130 140 150
PNESKHITYR QLHAEVCRMA NVLRNRGVGK GDRVTLYLPM IPEAAYAMLA
160 170 180 190 200
CARLGAIHAI VFGGFSPDSL ASRIKGCGSK LVITADEGLR GGRKVPLKAN
210 220 230 240 250
VDEAIKRLDK DLVDHVIVVK RTGGNVAMEP GRDVYYHEAA EQVTDECPAE
260 270 280 290 300
AVEAEHPLFI LYTSGSTGQP KGVVHTTGGY LVYASMTHQY VFDYHDGEVY
310 320 330 340 350
WCTADVGWVT GHSYIVYGPL ANGATTLMFE GIPTYPSNSR FWEVIDKHKV
360 370 380 390 400
NIFYTAPTAI RSLMGGGEGP VKKTSRQSLR VLGSVGEPIN PEAWDWYYRV
410 420 430 440 450
VGDSRCPIVD TWWQTETGGI LITPLPGATR LKPGSATLPF FGVQPVMVDA
460 470 480 490 500
EGKILDGACE GNLCIKDSWP GQMRTVYGDH ERFEQTYFST YKDLYFTGDG
510 520 530 540 550
ARRDADGYYW ITGRVDDVIN VSGHRMGTAE VESSLVAHPK VSEAAVVGYP
560 570 580 590 600
HNVKGQGIYA YVTLNEGEEG TDELRKELVT WVRKDIGPIA SPDLLQFAPG
610 620 630 640 650
LPKTRSGKIM RRILRKIAED DFGSLGDTST LAEPAVVDDL IENRQNRQNR

SA
Length:652
Mass (Da):72,190
Last modified:February 10, 2009 - v1
Checksum:i50BAC6E81B462479
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001298 Genomic DNA Translation: ACK83624.1
RefSeqiWP_015951093.1, NC_011757.1

Genome annotation databases

EnsemblBacteriaiACK83624; ACK83624; Mchl_2785
KEGGimch:Mchl_2785

Similar proteinsi

Entry informationi

Entry nameiACSA_METC4
AccessioniPrimary (citable) accession number: B7KPN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: May 23, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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