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Protein

Phosphoglycerate kinase

Gene

pgk

Organism
Methylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.UniRule annotation

Pathway: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (Mchl_1043), Pyruvate kinase (Mchl_2971)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381SubstrateUniRule annotation
Binding sitei120 – 1201SubstrateUniRule annotation
Binding sitei153 – 1531SubstrateUniRule annotation
Binding sitei203 – 2031ATPUniRule annotation
Binding sitei325 – 3251ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi355 – 3584ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMCHL440085:GCXT-2697-MONOMER.
UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinaseUniRule annotation (EC:2.7.2.3UniRule annotation)
Gene namesi
Name:pgkUniRule annotation
Ordered Locus Names:Mchl_2645
OrganismiMethylobacterium extorquens (strain CM4 / NCIMB 13688) (Methylobacterium chloromethanicum)
Taxonomic identifieri440085 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesMethylobacteriaceaeMethylobacterium
ProteomesiUP000002385 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Phosphoglycerate kinasePRO_1000203339Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB7KNP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 253Substrate bindingUniRule annotation
Regioni61 – 644Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227107.
KOiK00927.
OMAiCTIHLPL.
OrthoDBiEOG64N9Z0.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7KNP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDFRTLDDA GPLQGKRVLL RVDLNVPMEG GRVTDATRIE RVVPTIREIA
60 70 80 90 100
DAGGRVILLA HFGRPKGKPD PKDSLKPILS TLSEKLGRPV AFGEDCVGEA
110 120 130 140 150
AASAVAALKD GDVILLENTR YHAGEEKNDP DFAKALAANG DIYVNEAFSA
160 170 180 190 200
AHRAHASTEA LARLLPAYAG RLMQAELDAL TKGLEAPARP VIAIVGGAKV
210 220 230 240 250
STKIDLLENL VAKVDMLVIG GGMANTFLHA QGKDVGKSLC EKDLAETAKR
260 270 280 290 300
ILAAAKEKNC TIILPVDALV AREFRANAEN ETVPVDAVPS DAMILDVGAS
310 320 330 340 350
SIATIDGAIE EARTLVWNGP LGAFELTPFD TGTVAVAQHA ARRTKAGQLV
360 370 380 390 400
SVAGGGDTVA ALNHAGVGED FSYVSTAGGA FLEWLEGKEL PGVEALRAQG
Length:400
Mass (Da):41,833
Last modified:February 10, 2009 - v1
Checksum:iF799C424BAC988D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001298 Genomic DNA. Translation: ACK83485.1.
RefSeqiWP_015950997.1. NC_011757.1.
YP_002421413.1. NC_011757.1.

Genome annotation databases

EnsemblBacteriaiACK83485; ACK83485; Mchl_2645.
KEGGimch:Mchl_2645.
PATRICi22499537. VBIMetChl132133_2601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001298 Genomic DNA. Translation: ACK83485.1.
RefSeqiWP_015950997.1. NC_011757.1.
YP_002421413.1. NC_011757.1.

3D structure databases

ProteinModelPortaliB7KNP6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK83485; ACK83485; Mchl_2645.
KEGGimch:Mchl_2645.
PATRICi22499537. VBIMetChl132133_2601.

Phylogenomic databases

eggNOGiCOG0126.
HOGENOMiHOG000227107.
KOiK00927.
OMAiCTIHLPL.
OrthoDBiEOG64N9Z0.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.
BioCyciMCHL440085:GCXT-2697-MONOMER.

Family and domain databases

Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Methylobacterium chloromethanicum CM4."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Marx C., Richardson P.
    Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CM4 / NCIMB 13688.

Entry informationi

Entry nameiPGK_METC4
AccessioniPrimary (citable) accession number: B7KNP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 10, 2009
Last modified: June 24, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.