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B7KH06

- DAPF_CYAP7

UniProt

B7KH06 - DAPF_CYAP7

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Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131SubstrateUniRule annotation
Binding sitei47 – 471SubstrateUniRule annotation
Binding sitei66 – 661SubstrateUniRule annotation
Active sitei75 – 751Proton donor/acceptorUniRule annotation
Binding sitei164 – 1641SubstrateUniRule annotation
Sitei166 – 1661Important for catalytic activityUniRule annotation
Binding sitei197 – 1971SubstrateUniRule annotation
Sitei215 – 2151Important for catalytic activityUniRule annotation
Active sitei224 – 2241Proton donor/acceptorUniRule annotation

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciCSP65393:GJP7-5199-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
Short name:
DAP epimeraseUniRule annotation
Gene namesi
Name:dapFUniRule annotation
Ordered Locus Names:PCC7424_5143
OrganismiCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Taxonomic identifieri65393 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002384: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 281281Diaminopimelate epimerasePRO_1000118665Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi75 ↔ 224

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi65393.PCC7424_5143.

Structurei

3D structure databases

ProteinModelPortaliB7KH06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni10 – 112Substrate bindingUniRule annotation
Regioni75 – 773Substrate bindingUniRule annotation
Regioni215 – 2162Substrate bindingUniRule annotation
Regioni225 – 2262Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the diaminopimelate epimerase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220466.
KOiK01778.
OMAiLIVEPPY.
OrthoDBiEOG6ND0M5.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7KH06-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGIEFTKYHG LGNDFILIDN RHGSEPMVSP EMAVEMCDRH FGIGADGVIF
60 70 80 90 100
VLPGTSQTDY QMRIFNSDGS EPEMCGNGIR CLAQFIAELE KTTEVGKSYR
110 120 130 140 150
IDTLGGLMTP RLEAQEQVTV DMGLPRLLGS EIPTTLVSRT EKVIDQPIEV
160 170 180 190 200
AGQSWLVTCV SMGNPHCITF VEDVKSIALE TIGPQFEHHV AFPQRTNTEF
210 220 230 240 250
IEVLRPDYLK MRVWERGAGI TLACGTGACA SVVAGVLTGK SDRRCTVELP
260 270 280
GGCLSIYWSE TDGHVYMTGP AKRVFTGVYE M
Length:281
Mass (Da):30,849
Last modified:February 10, 2009 - v1
Checksum:iA6EDE352275FE151
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK73493.1.
RefSeqiWP_015957073.1. NC_011729.1.
YP_002380361.1. NC_011729.1.

Genome annotation databases

EnsemblBacteriaiACK73493; ACK73493; PCC7424_5143.
GeneIDi7108042.
KEGGicyc:PCC7424_5143.
PATRICi21559322. VBICyaSp136448_5252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK73493.1 .
RefSeqi WP_015957073.1. NC_011729.1.
YP_002380361.1. NC_011729.1.

3D structure databases

ProteinModelPortali B7KH06.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 65393.PCC7424_5143.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK73493 ; ACK73493 ; PCC7424_5143 .
GeneIDi 7108042.
KEGGi cyc:PCC7424_5143.
PATRICi 21559322. VBICyaSp136448_5252.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220466.
KOi K01778.
OMAi LIVEPPY.
OrthoDBi EOG6ND0M5.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci CSP65393:GJP7-5199-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7424.

Entry informationi

Entry nameiDAPF_CYAP7
AccessioniPrimary (citable) accession number: B7KH06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3