B7KFZ3 (DUT_CYAP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	PCC7424_0730

Organism

Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155)) [Complete proteome] [HAMAP]

Taxonomic identifier

65393 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Cyanothece

Protein attributes

Sequence length	145 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 145	145	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_1000117563
Regions
	Region	62 – 64	3	Substrate binding By similarity
	Region	79 – 81	3	Substrate binding By similarity
Sites
	Binding site	75	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7KFZ3 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 1F2E010BBA87AC57	FASTA	145	15,508
	10 20 30 40 50 60 MKLKVTKLEK AAILPKYVHS DDSGLDLSAI EDLEIPPGES QLVPTGIAIE LPPNTEAQIR 70 80 90 100 110 120 PRSGLALKHQ ITVLNTPGTV DEGYRGEIGV ILINHGKNSF KVTRGMKIAQ MVIAPVIRVE 130 140 VEEVDHLSDT TRGSGGFGST GLTSD

References

[1]

"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria." Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B. MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7424.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001291 Genomic DNA. Translation: ACK69186.1.
RefSeq	YP_002376054.1. NC_011729.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	65393.PCC7424_0730.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACK69186; ACK69186; PCC7424_0730.
GeneID	7109137.
KEGG	cyc:PCC7424_0730.
PATRIC	21550250. VBICyaSp136448_0762.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028966.
KO	K01520.
OMA	CLINLSN.
ProtClustDB	CLSK2328882.
Enzyme and pathway databases
BioCyc	CSP65393:GJP7-736-MONOMER.
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_CYAP7

Accession

Primary (citable) accession number: B7KFZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program