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B7KFT1

- SYI_CYAP7

UniProt

B7KFT1 - SYI_CYAP7

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
Binding sitei613 – 6131ATPUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation
Metal bindingi948 – 9481ZincUniRule annotation
Metal bindingi951 – 9511ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCSP65393:GJP7-5109-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PCC7424_5054
OrganismiCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Taxonomic identifieri65393 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002384: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Isoleucine--tRNA ligasePRO_1000189147Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi65393.PCC7424_5054.

Structurei

3D structure databases

ProteinModelPortaliB7KFT1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi610 – 6145"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7KFT1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEAKSYKDT VNLPQTKFDM RANAVKREPE LQKFWAENQI YETLSENNPG
60 70 80 90 100
DIFILHDGPP YANGSLHMGH ALNKTLKDII NKYKLQRGYK ARYVPGWDCH
110 120 130 140 150
GLPIELKVLQ SMKQKEREDL TPLKLRHKAR DFALSTQKEQ SESFQRFGVW
160 170 180 190 200
GDWEHPYLTL TPDYEAAQIG VFGQMALKGY IYRGLKPVHW SPSSQTALAE
210 220 230 240 250
AELEYPEGHT SRSVYAVFPI TKASDTAKKV LTPYLKDLGV AIWTTTPWTL
260 270 280 290 300
PGNLAVALNP DLTYAVVESG KPVCKHQYFI VAADLVDKLS ATFETPLTVK
310 320 330 340 350
ATLKGEDLEH TTYRHPLFDR ESEILIGGDY VTTESGTGLV HTAPGHGQED
360 370 380 390 400
YIVGQRYGLP VLSPVDEKGN FTSEAGQFAG LNVLKDANEA IIKELEEKGA
410 420 430 440 450
LLKEEPYQHK YPYDWRTKKP TIFRATEQWF ASVEGFRDTA LEAIKNVTWI
460 470 480 490 500
PPQGENRITP MVADRSDWCI SRQRSWGVPI PVFYDEETNE PLLNEETINH
510 520 530 540 550
VQAIFAEKGS DAWWELSVEE LLPQPYRNNG RKYRKGMDTM DVWFDSGSSW
560 570 580 590 600
AAVAKQRPEL SYPVDIYLEG SDQHRGWFQS SLLTSVATNG IAPYKMVLTH
610 620 630 640 650
GFVLDEQGRK MSKSIGNIVD PNMIINGGKD QKKEPAYGAD VLRLWVSSVD
660 670 680 690 700
YSSDVPIGQN ILKQLVDVRN KIRNTARFLL GNLHDFDPEK DTVAYEDLPE
710 720 730 740 750
LDRYMLHRIT EVFTEVTEAF ETFQFFRFFQ VVQNFCVVDL SNFYLDIAKD
760 770 780 790 800
RLYISDPNNL RRRSCQTVLK VALENLAKSI APVLCHLAED IWQFLPYKTP
810 820 830 840 850
YKSVFEAGWV ELESEWKRPE LTPKWSKFRQ IRDEVNKVME LARKDKMIGS
860 870 880 890 900
SLDAKVLLYV SDKELREHLD SFNPSDSLSN NRVDELRYLF LASQVDLVDS
910 920 930 940 950
PKTIGKANYK SESETLGVAI VKADGEKCDR CWNYSTHVGE FVDDPTLCER

CNAALKGEF
Length:959
Mass (Da):109,390
Last modified:February 10, 2009 - v1
Checksum:i69BC3E8FB4EF089F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK73406.1.
RefSeqiWP_015956986.1. NC_011729.1.
YP_002380274.1. NC_011729.1.

Genome annotation databases

EnsemblBacteriaiACK73406; ACK73406; PCC7424_5054.
GeneIDi7107200.
KEGGicyc:PCC7424_5054.
PATRICi21559142. VBICyaSp136448_5163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK73406.1 .
RefSeqi WP_015956986.1. NC_011729.1.
YP_002380274.1. NC_011729.1.

3D structure databases

ProteinModelPortali B7KFT1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 65393.PCC7424_5054.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK73406 ; ACK73406 ; PCC7424_5054 .
GeneIDi 7107200.
KEGGi cyc:PCC7424_5054.
PATRICi 21559142. VBICyaSp136448_5163.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CSP65393:GJP7-5109-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7424.

Entry informationi

Entry nameiSYI_CYAP7
AccessioniPrimary (citable) accession number: B7KFT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: November 26, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3