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B7KFT1

- SYI_CYAP7

UniProt

B7KFT1 - SYI_CYAP7

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Protein
Isoleucine--tRNA ligase
Gene
ileS, PCC7424_5054
Organism
Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylate By similarity
Binding sitei613 – 6131ATP By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity
Metal bindingi948 – 9481Zinc By similarity
Metal bindingi951 – 9511Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCSP65393:GJP7-5109-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PCC7424_5054
OrganismiCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Taxonomic identifieri65393 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002384: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189147Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi65393.PCC7424_5054.

Structurei

3D structure databases

ProteinModelPortaliB7KFT1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi610 – 6145"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7KFT1-1 [UniParc]FASTAAdd to Basket

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MTEAKSYKDT VNLPQTKFDM RANAVKREPE LQKFWAENQI YETLSENNPG    50
DIFILHDGPP YANGSLHMGH ALNKTLKDII NKYKLQRGYK ARYVPGWDCH 100
GLPIELKVLQ SMKQKEREDL TPLKLRHKAR DFALSTQKEQ SESFQRFGVW 150
GDWEHPYLTL TPDYEAAQIG VFGQMALKGY IYRGLKPVHW SPSSQTALAE 200
AELEYPEGHT SRSVYAVFPI TKASDTAKKV LTPYLKDLGV AIWTTTPWTL 250
PGNLAVALNP DLTYAVVESG KPVCKHQYFI VAADLVDKLS ATFETPLTVK 300
ATLKGEDLEH TTYRHPLFDR ESEILIGGDY VTTESGTGLV HTAPGHGQED 350
YIVGQRYGLP VLSPVDEKGN FTSEAGQFAG LNVLKDANEA IIKELEEKGA 400
LLKEEPYQHK YPYDWRTKKP TIFRATEQWF ASVEGFRDTA LEAIKNVTWI 450
PPQGENRITP MVADRSDWCI SRQRSWGVPI PVFYDEETNE PLLNEETINH 500
VQAIFAEKGS DAWWELSVEE LLPQPYRNNG RKYRKGMDTM DVWFDSGSSW 550
AAVAKQRPEL SYPVDIYLEG SDQHRGWFQS SLLTSVATNG IAPYKMVLTH 600
GFVLDEQGRK MSKSIGNIVD PNMIINGGKD QKKEPAYGAD VLRLWVSSVD 650
YSSDVPIGQN ILKQLVDVRN KIRNTARFLL GNLHDFDPEK DTVAYEDLPE 700
LDRYMLHRIT EVFTEVTEAF ETFQFFRFFQ VVQNFCVVDL SNFYLDIAKD 750
RLYISDPNNL RRRSCQTVLK VALENLAKSI APVLCHLAED IWQFLPYKTP 800
YKSVFEAGWV ELESEWKRPE LTPKWSKFRQ IRDEVNKVME LARKDKMIGS 850
SLDAKVLLYV SDKELREHLD SFNPSDSLSN NRVDELRYLF LASQVDLVDS 900
PKTIGKANYK SESETLGVAI VKADGEKCDR CWNYSTHVGE FVDDPTLCER 950
CNAALKGEF 959
Length:959
Mass (Da):109,390
Last modified:February 10, 2009 - v1
Checksum:i69BC3E8FB4EF089F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001291 Genomic DNA. Translation: ACK73406.1.
RefSeqiWP_015956986.1. NC_011729.1.
YP_002380274.1. NC_011729.1.

Genome annotation databases

EnsemblBacteriaiACK73406; ACK73406; PCC7424_5054.
GeneIDi7107200.
KEGGicyc:PCC7424_5054.
PATRICi21559142. VBICyaSp136448_5163.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001291 Genomic DNA. Translation: ACK73406.1 .
RefSeqi WP_015956986.1. NC_011729.1.
YP_002380274.1. NC_011729.1.

3D structure databases

ProteinModelPortali B7KFT1.
ModBasei Search...

Protein-protein interaction databases

STRINGi 65393.PCC7424_5054.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK73406 ; ACK73406 ; PCC7424_5054 .
GeneIDi 7107200.
KEGGi cyc:PCC7424_5054.
PATRICi 21559142. VBICyaSp136448_5163.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CSP65393:GJP7-5109-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7424.

Entry informationi

Entry nameiSYI_CYAP7
AccessioniPrimary (citable) accession number: B7KFT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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