Reviewed, UniProtKB/Swiss-Prot B7KFS2 (LPXA_CYAP7)

Names and origin

Protein names

Recommended name:
    Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase
      Short name=UDP-N-acetylglucosamine acyltransferase
    EC=2.3.1.129

Gene names

Name:	lpxA
Ordered Locus Names:	PCC7424_5045

Organism

Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155)) [Complete proteome] [HAMAP]

Taxonomic identifier

65393 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00387
Catalytic activity	(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetylglucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine. HAMAP MF_00387
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 1/6. HAMAP MF_00387
Subunit structure	Homotrimer By similarity. HAMAP MF_00387
Subcellular location	Cytoplasm By similarity HAMAP MF_00387.
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxA subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase HAMAP MF_00387		PRO_1000122698

Sequences

	Sequence		Length	Mass (Da)
	B7KFS2-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 3CC0FA37813CC9BD	FASTA	276	30,010
	10 20 30 40 50 60 MLINIRPGDA PLSTPIHPTA IIHPNAELHP SVQVAPYAVI GEQVKIGAST IIGPNVVIEG 70 80 90 100 110 120 PTEIGVGNRI FAGAVIGTEP QDLKYRGAAS QVKIGDHNQI REYVTINRAT GENEVTQIGN 130 140 150 160 170 180 NNLLMAYAHV AHNCVIEDEV IIANSVALAG HIYIESKARI SGVLGVHQFV HIGRLAMVGG 190 200 210 220 230 240 MARIERDVPP FTTVEGNPSR VRTLNLIGLK RAGVNEAEIS EMKKAFRLIY RSNLTLKQAL 250 260 270 EQLESWSNNP YVQHLRDFLH QSTTVTGRRG PIPGKE

References

[1]

"Complete sequence of chromosome Cyanothece sp. PCC 7424." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001291 Genomic DNA. Translation: ACK73397.1.
RefSeq	YP_002380265.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7107191.
GenomeReviews	Gene locus PCC7424_5045 in contig CP001291_GR.
KEGG	cyc:PCC7424_5045.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG659295.
Family and domain databases
HAMAP	MF_00387. LpxA. [Tree]
InterPro	IPR010137. Lipid_A_lpxA. IPR011004. Trimer_LpxA-like. [Graphical view]
PIRSF	PIRSF000456. UDP-GlcNAc_acltr. 1 hit.
TIGRFAMs	TIGR01852. lipid_A_lpxA. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXA_CYAP7

Accession

Primary (citable) accession number: B7KFS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	February 9, 2010
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)