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Protein

Biotin synthase

Gene

bioB

Organism
Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi85Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi89Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi92Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi130Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi167Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi227Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi297Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSP65393:G1GUC-4933-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:PCC7424_4971
OrganismiCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Taxonomic identifieri65393 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesCyanothecaceaeCyanothece
Proteomesi
  • UP000002384 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003813391 – 359Biotin synthaseAdd BLAST359

Proteomic databases

PRIDEiB7KFJ9

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi65393.PCC7424_4971

Structurei

3D structure databases

ProteinModelPortaliB7KFJ9
SMRiB7KFJ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QSQ Bacteria
COG0502 LUCA
HOGENOMiHOG000239958
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

B7KFJ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQASLSSPI TPPKDTQALQ EWLHQLADRI ISGYRITKVE ALALTEIEGQ
60 70 80 90 100
DQILLLCEAA DRIRQACCGN RVDLCSIINI KSGHCSENCS FCSQSVHHPG
110 120 130 140 150
QDSPVYGLKT SEEIVQQAKA AAAAGAKRFC LVSQGRGLKY NSPKSKEFAE
160 170 180 190 200
ILATVKRITT EAKIKPCCAL GELTLEQAQA LKEAGVTRYN HNLEASATFY
210 220 230 240 250
PQIVTTHTWA DRVETVKNLK AAGIQACTGG IIGMGESWED RIDLALSLRD
260 270 280 290 300
LEVDSVPINL LNPRQGTPLG HLPKLDPFEA LQAIAIFRFI LPQQILRYAG
310 320 330 340 350
GREAIMGELQ SLGLKAGINA MLIGHYLTTL GQSPQQDQAM LKSLGLEGGE

APIPGEYQP
Length:359
Mass (Da):38,939
Last modified:February 10, 2009 - v1
Checksum:i380CEEFCA5ADC892
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA Translation: ACK73324.1
RefSeqiWP_015956905.1, NC_011729.1

Genome annotation databases

EnsemblBacteriaiACK73324; ACK73324; PCC7424_4971
KEGGicyc:PCC7424_4971

Similar proteinsi

Entry informationi

Entry nameiBIOB_CYAP7
AccessioniPrimary (citable) accession number: B7KFJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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