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B7KFJ9

- BIOB_CYAP7

UniProt

B7KFJ9 - BIOB_CYAP7

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Protein

Biotin synthase

Gene
bioB, PCC7424_4971
Organism
Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi85 – 851Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi130 – 1301Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi167 – 1671Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi227 – 2271Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi297 – 2971Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSP65393:GJP7-5026-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:PCC7424_4971
OrganismiCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Taxonomic identifieri65393 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002384: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Biotin synthaseUniRule annotationPRO_0000381339Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi65393.PCC7424_4971.

Structurei

3D structure databases

ProteinModelPortaliB7KFJ9.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B7KFJ9-1 [UniParc]FASTAAdd to Basket

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MVQASLSSPI TPPKDTQALQ EWLHQLADRI ISGYRITKVE ALALTEIEGQ    50
DQILLLCEAA DRIRQACCGN RVDLCSIINI KSGHCSENCS FCSQSVHHPG 100
QDSPVYGLKT SEEIVQQAKA AAAAGAKRFC LVSQGRGLKY NSPKSKEFAE 150
ILATVKRITT EAKIKPCCAL GELTLEQAQA LKEAGVTRYN HNLEASATFY 200
PQIVTTHTWA DRVETVKNLK AAGIQACTGG IIGMGESWED RIDLALSLRD 250
LEVDSVPINL LNPRQGTPLG HLPKLDPFEA LQAIAIFRFI LPQQILRYAG 300
GREAIMGELQ SLGLKAGINA MLIGHYLTTL GQSPQQDQAM LKSLGLEGGE 350
APIPGEYQP 359
Length:359
Mass (Da):38,939
Last modified:February 10, 2009 - v1
Checksum:i380CEEFCA5ADC892
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001291 Genomic DNA. Translation: ACK73324.1.
RefSeqiWP_015956905.1. NC_011729.1.
YP_002380192.1. NC_011729.1.

Genome annotation databases

EnsemblBacteriaiACK73324; ACK73324; PCC7424_4971.
GeneIDi7107037.
KEGGicyc:PCC7424_4971.
PATRICi21558980. VBICyaSp136448_5082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001291 Genomic DNA. Translation: ACK73324.1 .
RefSeqi WP_015956905.1. NC_011729.1.
YP_002380192.1. NC_011729.1.

3D structure databases

ProteinModelPortali B7KFJ9.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 65393.PCC7424_4971.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK73324 ; ACK73324 ; PCC7424_4971 .
GeneIDi 7107037.
KEGGi cyc:PCC7424_4971.
PATRICi 21558980. VBICyaSp136448_5082.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CSP65393:GJP7-5026-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7424.

Entry informationi

Entry nameiBIOB_CYAP7
AccessioniPrimary (citable) accession number: B7KFJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: September 3, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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