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B7KE00 (B7KE00_CYAP7) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione synthetase HAMAP-Rule MF_00162
EC=6.3.2.3 HAMAP-Rule MF_00162
Alternative name(s):
GSH synthetase HAMAP-Rule MF_00162
Glutathione synthase HAMAP-Rule MF_00162
Gene names
Name:gshB HAMAP-Rule MF_00162
Ordered Locus Names:PCC7424_3299 EMBL ACK71698.1
OrganismCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155)) [Complete proteome] [HAMAP] EMBL ACK71698.1
Taxonomic identifier65393 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Cyanothece

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds (magnesium or manganese,ion) per subunit By similarity. HAMAP-Rule MF_00162

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family. HAMAP-Rule MF_00162

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00162

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain133 – 317185ATP-grasp By similarity HAMAP-Rule MF_00162
Nucleotide binding159 – 21557ATP By similarity HAMAP-Rule MF_00162

Sites

Metal binding2881Magnesium or manganese By similarity HAMAP-Rule MF_00162
Metal binding2901Magnesium or manganese By similarity HAMAP-Rule MF_00162

Sequences

Sequence LengthMass (Da)Tools
B7KE00 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 57C27C78E2683290

FASTA32035,720
        10         20         30         40         50         60 
MKFVFIIDPL AKLDPGHDTS VALMEAAQSL GHEIWVTEIH QLSVINGKAC AILHQVELTP 

        70         80         90        100        110        120 
VELRENRWIA VSLWYQVSEG VVTNLEEMDA VFMRKDPPVT IQYLYATYIL ALIDESKTLV 

       130        140        150        160        170        180 
INSPRGLREA NEKMYTLQFH QVMPETVVSL DKKIIRRFVE EKKAAVLKPL GGKAGEGILF 

       190        200        210        220        230        240 
LEPGDRNFNS IIEVSTKHGQ EPVMVQDYLP AAKEGDKRII LLNGEPIGAV NRVPTGAEFR 

       250        260        270        280        290        300 
GNMAVGGRVE KTEITAREKE ICALVGPKLR EDGLYFVGLD VIGGYLTEVN VTSPTGVREI 

       310        320 
DRLNNVSLGK QVIQWLENYK

« Hide

References

[1]"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7424.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001291 Genomic DNA. Translation: ACK71698.1.
RefSeqYP_002378566.1. NC_011729.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRING65393.PCC7424_3299.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK71698; ACK71698; PCC7424_3299.
GeneID7111908.
KEGGcyc:PCC7424_3299.
PATRIC21555558. VBICyaSp136448_3384.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAWMRKDPP.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycCSP65393:GJP7-3339-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB7KE00_CYAP7
AccessionPrimary (citable) accession number: B7KE00
Entry history
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info