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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei618 – 6181ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSP65393:GJP7-1882-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:PCC7424_1860
OrganismiCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Taxonomic identifieri65393 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002384: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 854854Leucine--tRNA ligasePRO_1000199189Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi65393.PCC7424_1860.

Structurei

3D structure databases

ProteinModelPortaliB7KCI7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi615 – 6195"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7KCI7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESVYNAEAI EKKWQQTWVE MGLDQTPEDN TKPKYYALSM FPYPSGNLHM
60 70 80 90 100
GHVRNYVITD VIARLKRMQG HRVLHPMGWD AFGLPAENAA IERGIHPAKW
110 120 130 140 150
TYQNIAQMRQ QLQQLGLSID WSREVTTCSP DYYQWTQWLF LQFFQAGLAY
160 170 180 190 200
QKEAAVNWDP VDQTVLANEQ VDSEGKSWRS GAKVERKMLR QWFLKITDYA
210 220 230 240 250
EQLLNDLDKL PGWPEKVKLM QANWIGKSIG AYLEFPIVGM DEKIAVFTTR
260 270 280 290 300
PDTVYGVTYV VLAPEHPLTP KVTTAKNKKA VEKFIKEVTS ESEQERTAED
310 320 330 340 350
KPKKGILTGG KAINPFNGQE VPILIANYVL YEYGTGAVMG VPAHDIRDFK
360 370 380 390 400
FAQENKLPIR VVIVPEDQQD QDVTLTQAYT EPGIMVNSGS FDGMDSITGK
410 420 430 440 450
TAIIDYAEKQ GFGKARVQYR LRDWLISRQR YWGCPIPVIH CPSCGTVPVP
460 470 480 490 500
DEDLPVKLPE DVEFSGRGAS PLAKLDSWIN VPCPSCGEPA KRETDTMDTF
510 520 530 540 550
IDSSWYFLRY TDANDQQTAF SLDKVNDWMS VDQYVGGVEH AILHLLYSRF
560 570 580 590 600
FTKVVRDRGL VDVDEPFQRL LTQGMVQGMT YKNPKTGKYI PSSQVNPEDP
610 620 630 640 650
KDPETGEPLS VFYEKMSKSK YNGVDPKQVL AKYGADTARM FILFKAPPEK
660 670 680 690 700
DLEWDDADVE GQFRFLNKVW RIVGEYQSNH KSSSKKKGSL TKVEKDLRRA
710 720 730 740 750
IHIAIKEITE DLDGEYQFNT AVSELMKLSN ALVDAKCYES PVYTEGVQTL
760 770 780 790 800
LILLAPFAPH IAEELWHTLG HSESVHLQGW PQLDPSALEV DEITLVIQIM
810 820 830 840 850
GKTRGTIQVP SSATKEELEK FAFESEVAQR HLKDQEVKKV IVVPGKLVNF

VVGK
Length:854
Mass (Da):96,892
Last modified:February 10, 2009 - v1
Checksum:iE7E26E0AA0BBCC43
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK70292.1.
RefSeqiWP_012599235.1. NC_011729.1.
YP_002377160.1. NC_011729.1.

Genome annotation databases

EnsemblBacteriaiACK70292; ACK70292; PCC7424_1860.
GeneIDi7109861.
KEGGicyc:PCC7424_1860.
PATRICi21552570. VBICyaSp136448_1907.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK70292.1.
RefSeqiWP_012599235.1. NC_011729.1.
YP_002377160.1. NC_011729.1.

3D structure databases

ProteinModelPortaliB7KCI7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi65393.PCC7424_1860.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK70292; ACK70292; PCC7424_1860.
GeneIDi7109861.
KEGGicyc:PCC7424_1860.
PATRICi21552570. VBICyaSp136448_1907.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCSP65393:GJP7-1882-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7424.

Entry informationi

Entry nameiSYL_CYAP7
AccessioniPrimary (citable) accession number: B7KCI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: January 7, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.