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B7KCI7 (SYL_CYAP7) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PCC7424_1860
OrganismCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155)) [Complete proteome] [HAMAP]
Taxonomic identifier65393 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece

Protein attributes

Sequence length854 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 854854Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199189

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif615 – 6195"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6181ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7KCI7 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: E7E26E0AA0BBCC43

FASTA85496,892
        10         20         30         40         50         60 
MESVYNAEAI EKKWQQTWVE MGLDQTPEDN TKPKYYALSM FPYPSGNLHM GHVRNYVITD 

        70         80         90        100        110        120 
VIARLKRMQG HRVLHPMGWD AFGLPAENAA IERGIHPAKW TYQNIAQMRQ QLQQLGLSID 

       130        140        150        160        170        180 
WSREVTTCSP DYYQWTQWLF LQFFQAGLAY QKEAAVNWDP VDQTVLANEQ VDSEGKSWRS 

       190        200        210        220        230        240 
GAKVERKMLR QWFLKITDYA EQLLNDLDKL PGWPEKVKLM QANWIGKSIG AYLEFPIVGM 

       250        260        270        280        290        300 
DEKIAVFTTR PDTVYGVTYV VLAPEHPLTP KVTTAKNKKA VEKFIKEVTS ESEQERTAED 

       310        320        330        340        350        360 
KPKKGILTGG KAINPFNGQE VPILIANYVL YEYGTGAVMG VPAHDIRDFK FAQENKLPIR 

       370        380        390        400        410        420 
VVIVPEDQQD QDVTLTQAYT EPGIMVNSGS FDGMDSITGK TAIIDYAEKQ GFGKARVQYR 

       430        440        450        460        470        480 
LRDWLISRQR YWGCPIPVIH CPSCGTVPVP DEDLPVKLPE DVEFSGRGAS PLAKLDSWIN 

       490        500        510        520        530        540 
VPCPSCGEPA KRETDTMDTF IDSSWYFLRY TDANDQQTAF SLDKVNDWMS VDQYVGGVEH 

       550        560        570        580        590        600 
AILHLLYSRF FTKVVRDRGL VDVDEPFQRL LTQGMVQGMT YKNPKTGKYI PSSQVNPEDP 

       610        620        630        640        650        660 
KDPETGEPLS VFYEKMSKSK YNGVDPKQVL AKYGADTARM FILFKAPPEK DLEWDDADVE 

       670        680        690        700        710        720 
GQFRFLNKVW RIVGEYQSNH KSSSKKKGSL TKVEKDLRRA IHIAIKEITE DLDGEYQFNT 

       730        740        750        760        770        780 
AVSELMKLSN ALVDAKCYES PVYTEGVQTL LILLAPFAPH IAEELWHTLG HSESVHLQGW 

       790        800        810        820        830        840 
PQLDPSALEV DEITLVIQIM GKTRGTIQVP SSATKEELEK FAFESEVAQR HLKDQEVKKV 

       850 
IVVPGKLVNF VVGK 

« Hide

References

[1]"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7424.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001291 Genomic DNA. Translation: ACK70292.1.
RefSeqYP_002377160.1. NC_011729.1.

3D structure databases

ProteinModelPortalB7KCI7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING65393.PCC7424_1860.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK70292; ACK70292; PCC7424_1860.
GeneID7109861.
KEGGcyc:PCC7424_1860.
PATRIC21552570. VBICyaSp136448_1907.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCSP65393:GJP7-1882-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CYAP7
AccessionPrimary (citable) accession number: B7KCI7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries