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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107ATPUniRule annotation1
Active sitei316Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi290 – 294ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:PCC7424_1826
OrganismiCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Taxonomic identifieri65393 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesCyanothecaceaeCyanothece
Proteomesi
  • UP000002384 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001950951 – 350RNA 3'-terminal phosphate cyclaseAdd BLAST350

Interactioni

Protein-protein interaction databases

STRINGi65393.PCC7424_1826.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKX. Bacteria.
COG0430. LUCA.
HOGENOMiHOG000015264.
KOiK01974.
OMAiNGCVDEF.
OrthoDBiPOG091H0UB7.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC. 1 hit.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PTHR11096:SF0. PTHR11096:SF0. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.

Sequencei

Sequence statusi: Complete.

B7KCF3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDIDGSWGE GGGQILRTTL SLSAITGQPI RLYQIRAGRQ KPGLSAQHLT
60 70 80 90 100
CVRAAATICN AEVRGDKLRS TLLEFIPTRP PQAGHYTFDV MDAQEGGSAG
110 120 130 140 150
AVTLILQTIL LPLAIASEES TVILKGGTHV AWSPPISYIE QVYLPLLSKL
160 170 180 190 200
GLQTELHLQA WGWYPQGGGE VHLHIKGNCQ LQGVELLERG ALKQVKGLAV
210 220 230 240 250
VTELPSHIPQ RMAMRCEKLL QQAHVKGYVQ PLRAKGVAPG AGVFLTSEYE
260 270 280 290 300
HICAGFAALG RRGLPAESVA QNAVEQLLSF HQQDAPVEEF LGDQLLLPMI
310 320 330 340 350
LAQSPSQYRV AHISEHLKTN VQTISQFGLA QIDLNLEDRL VFVKPGHNLT
Length:350
Mass (Da):38,007
Last modified:February 10, 2009 - v1
Checksum:i57E3DBE955CAACDC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK70258.1.
RefSeqiWP_012599201.1. NC_011729.1.

Genome annotation databases

EnsemblBacteriaiACK70258; ACK70258; PCC7424_1826.
KEGGicyc:PCC7424_1826.
PATRICi21552498. VBICyaSp136448_1871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK70258.1.
RefSeqiWP_012599201.1. NC_011729.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi65393.PCC7424_1826.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK70258; ACK70258; PCC7424_1826.
KEGGicyc:PCC7424_1826.
PATRICi21552498. VBICyaSp136448_1871.

Phylogenomic databases

eggNOGiENOG4105CKX. Bacteria.
COG0430. LUCA.
HOGENOMiHOG000015264.
KOiK01974.
OMAiNGCVDEF.
OrthoDBiPOG091H0UB7.

Family and domain databases

Gene3Di3.30.360.20. 1 hit.
3.65.10.20. 2 hits.
HAMAPiMF_00200. RTC. 1 hit.
InterProiIPR013791. RNA3'-term_phos_cycl_insert.
IPR023797. RNA3'_phos_cyclase_dom.
IPR000228. RNA3'_term_phos_cyc.
IPR017770. RNA3'_term_phos_cyc_type_1.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PANTHERiPTHR11096. PTHR11096. 1 hit.
PTHR11096:SF0. PTHR11096:SF0. 1 hit.
PfamiPF01137. RTC. 1 hit.
PF05189. RTC_insert. 1 hit.
[Graphical view]
PIRSFiPIRSF005378. RNA3'_term_phos_cycl_euk. 1 hit.
SUPFAMiSSF52913. SSF52913. 1 hit.
SSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRTCA_CYAP7
AccessioniPrimary (citable) accession number: B7KCF3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: November 2, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.