Reviewed, UniProtKB/Swiss-Prot B7KCF3 (RTCA_CYAP7)

Names and origin

Protein names

Recommended name:
    Probable RNA 3'-terminal phosphate cyclase
      Short name=RNA-3'-phosphate cyclase
      Short name=RNA cyclase
    EC=6.5.1.4

Gene names

Name:	rtcA
Ordered Locus Names:	PCC7424_1826

Organism

Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155)) [Complete proteome] [HAMAP]

Taxonomic identifier

65393 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) the enzyme acts on RNA-N3'P to produce RNA-N3'PP5'A; (C) a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity.
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP MF_00200
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Probable RNA 3'-terminal phosphate cyclase HAMAP MF_00200		PRO_1000195095
Sites
	Active site	316	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7KCF3-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 57E3DBE955CAACDC	FASTA	350	38,007
	10 20 30 40 50 60 MLDIDGSWGE GGGQILRTTL SLSAITGQPI RLYQIRAGRQ KPGLSAQHLT CVRAAATICN 70 80 90 100 110 120 AEVRGDKLRS TLLEFIPTRP PQAGHYTFDV MDAQEGGSAG AVTLILQTIL LPLAIASEES 130 140 150 160 170 180 TVILKGGTHV AWSPPISYIE QVYLPLLSKL GLQTELHLQA WGWYPQGGGE VHLHIKGNCQ 190 200 210 220 230 240 LQGVELLERG ALKQVKGLAV VTELPSHIPQ RMAMRCEKLL QQAHVKGYVQ PLRAKGVAPG 250 260 270 280 290 300 AGVFLTSEYE HICAGFAALG RRGLPAESVA QNAVEQLLSF HQQDAPVEEF LGDQLLLPMI 310 320 330 340 350 LAQSPSQYRV AHISEHLKTN VQTISQFGLA QIDLNLEDRL VFVKPGHNLT

References

[1]

"Complete sequence of chromosome Cyanothece sp. PCC 7424." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001291 Genomic DNA. Translation: ACK70258.1.
RefSeq	YP_002377126.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7109827.
GenomeReviews	Gene locus PCC7424_1826 in contig CP001291_GR.
KEGG	cyc:PCC7424_1826.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00200. [Tree]
InterPro	IPR000228. RNA3'_term_phos_cycl-like. IPR013796. RNA3'_term_phos_cycl_insert. IPR020723. RNA3'_term_phos_cycl_prd. IPR017770. RNA3'_term_phos_cycl_sub. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.20. RNA3'_term_phos_cycl. 1 hit.
PANTHER	PTHR11096. RNA3'_term_phos_cycl. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
PROSITE	PS01287. RTC. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RTCA_CYAP7

Accession

Primary (citable) accession number: B7KCF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	November 24, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)