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Protein

4-hydroxythreonine-4-phosphate dehydrogenase

Gene

pdxA

Organism
Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).UniRule annotation

Catalytic activityi

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei135 – 1351SubstrateUniRule annotation
Metal bindingi170 – 1701Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi215 – 2151Divalent metal cation; shared with dimeric partnerUniRule annotation
Metal bindingi282 – 2821Divalent metal cation; shared with dimeric partnerUniRule annotation
Binding sitei290 – 2901SubstrateUniRule annotation
Binding sitei299 – 2991SubstrateUniRule annotation
Binding sitei308 – 3081SubstrateUniRule annotation

GO - Molecular functioni

  1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD binding Source: InterPro

GO - Biological processi

  1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
  2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Pyridoxine biosynthesis

Keywords - Ligandi

Metal-binding, NAD, NADP

Enzyme and pathway databases

BioCyciCSP65393:GJP7-4569-MONOMER.
UniPathwayiUPA00244; UER00312.

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenaseUniRule annotation (EC:1.1.1.262UniRule annotation)
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenaseUniRule annotation
Gene namesi
Name:pdxAUniRule annotation
Ordered Locus Names:PCC7424_4519
OrganismiCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155))
Taxonomic identifieri65393 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000002384 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3433434-hydroxythreonine-4-phosphate dehydrogenasePRO_1000128240Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi65393.PCC7424_4519.

Structurei

3D structure databases

ProteinModelPortaliB7KAA8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PdxA family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221591.
KOiK00097.
OMAiCELADDK.
OrthoDBiEOG6GN6ZC.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.

Sequencei

Sequence statusi: Complete.

B7KAA8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSLALTLGD PAGIGSEVIL KALADPSVGE NYNITVVGSR SLLEESYHQL
60 70 80 90 100
RSLPHVNHSD LVDPANLLIL DIPLDKTTKS QIQLGKGNAA SGEASFTYLQ
110 120 130 140 150
AAIAGTLKGE FAGIVTAPIA KSCWKAAGYH YPGQTEVLAQ AAGVEKFGML
160 170 180 190 200
FVARSPYTGW MLRTLLATTH IPLSQVPTVL TPELMTLKLE LLIDCLKNDF
210 220 230 240 250
RLDHPKIAIA GLNPHSGEQG QLGTEERDWL TPWLEEEKKR HCEVELVGLV
260 270 280 290 300
PPDTMWVTPG QAWYGNAPHP QHGADGYLAL YHDQGLIPVK LMAFDQAINT
310 320 330 340
TIGLPFIRTS PDHGTAFDIA GKGIARPASM IEAIKLAQAL VNG
Length:343
Mass (Da):36,976
Last modified:February 9, 2009 - v1
Checksum:i9E479E2A31053509
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK72882.1.
RefSeqiWP_015956465.1. NC_011729.1.
YP_002379750.1. NC_011729.1.

Genome annotation databases

EnsemblBacteriaiACK72882; ACK72882; PCC7424_4519.
KEGGicyc:PCC7424_4519.
PATRICi21558056. VBICyaSp136448_4624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001291 Genomic DNA. Translation: ACK72882.1.
RefSeqiWP_015956465.1. NC_011729.1.
YP_002379750.1. NC_011729.1.

3D structure databases

ProteinModelPortaliB7KAA8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi65393.PCC7424_4519.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK72882; ACK72882; PCC7424_4519.
KEGGicyc:PCC7424_4519.
PATRICi21558056. VBICyaSp136448_4624.

Phylogenomic databases

eggNOGiCOG1995.
HOGENOMiHOG000221591.
KOiK00097.
OMAiCELADDK.
OrthoDBiEOG6GN6ZC.

Enzyme and pathway databases

UniPathwayiUPA00244; UER00312.
BioCyciCSP65393:GJP7-4569-MONOMER.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
HAMAPiMF_00536. PdxA.
InterProiIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamiPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00557. pdxA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 7424.

Entry informationi

Entry nameiPDXA_CYAP7
AccessioniPrimary (citable) accession number: B7KAA8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2009
Last sequence update: February 9, 2009
Last modified: March 31, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The active site is located at the dimer interface.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.