Reviewed, UniProtKB/Swiss-Prot B7K8X6 (SAHH_CYAP7)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	PCC7424_4381

Organism

Cyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155)) [Complete proteome] [HAMAP]

Taxonomic identifier

65393 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Cyanothece

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000129281
Regions
	Region	188 – 355	168	NAD binding By similarity
Sites
	Binding site	64	1	Substrate By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	161	1	Substrate By similarity
	Binding site	191	1	Substrate By similarity
	Binding site	195	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7K8X6-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: D9EC772C6FBE8809	FASTA	429	46,410
	10 20 30 40 50 60 MTVTPIRAKE ELKYEIKDIS LAPSGRQRIE WAGREMPVVK QIKDRFEKEK PFEGIRLIAC 70 80 90 100 110 120 CHVTTETANL AIALKAGGAD AVLIASNPLS TQDDVAACLV ADYGIPVFAI KGEDNATYHR 130 140 150 160 170 180 HVQIALDHKP NLIIDDGGDV TATLIQERKD QIADIIGTTE ETTTGIVRLA AMLKDGVLSF 190 200 210 220 230 240 PAMNVNDADT KHFFDNRYGT GQSTLDGIIR ATNVLLAGKT VVVAGYGWCG KGTAMRARGL 250 260 270 280 290 300 GANVIVTEIN AVKAIEAVMD GFRVMPMIEA APLGDLFVTV TGNKHVIRPE HFDVMKDGAI 310 320 330 340 350 360 VCNSGHFDIE IDLQSLGEKA SEIKEVRNFT QQYTLASGKS IVVLGEGRLI NLAAAEGHPS 370 380 390 400 410 420 AVMDMSFANQ ALACEYLVKN KGKLEPGIYN IPTEIDQEIA RLKLQGMGIS IDSLTPEQEA YLNSWTSGT

References

[1]

"Complete sequence of chromosome Cyanothece sp. PCC 7424." Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. , Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001291 Genomic DNA. Translation: ACK72745.1.
RefSeq	YP_002379613.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7111104.
GenomeReviews	Gene locus PCC7424_4381 in contig CP001291_GR.
KEGG	cyc:PCC7424_4381.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_CYAP7

Accession

Primary (citable) accession number: B7K8X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	September 22, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)