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B7K7P3 (RBL_CYAP7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase large chain

Short name=RuBisCO large subunit
EC=4.1.1.39
Gene names
Name:cbbL
Synonyms:rbcL
Ordered Locus Names:PCC7424_1367
OrganismCyanothece sp. (strain PCC 7424) (Synechococcus sp. (strain ATCC 29155)) [Complete proteome] [HAMAP]
Taxonomic identifier65393 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01338

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains By similarity.

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity.

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 472472Ribulose bisphosphate carboxylase large chain HAMAP-Rule MF_01338
PRO_1000142749

Sites

Active site1721Proton acceptor By similarity
Active site2911Proton acceptor By similarity
Metal binding1981Magnesium; via carbamate group By similarity
Metal binding2001Magnesium By similarity
Metal binding2011Magnesium By similarity
Binding site1201Substrate; in homodimeric partner By similarity
Binding site1701Substrate By similarity
Binding site1741Substrate By similarity
Binding site2921Substrate By similarity
Binding site3241Substrate By similarity
Binding site3761Substrate By similarity
Site3311Transition state stabilizer By similarity

Amino acid modifications

Modified residue1981N6-carboxylysine By similarity

Sequences

Sequence LengthMass (Da)Tools
B7K7P3 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: BCB9C7B95BE45189

FASTA47252,740
        10         20         30         40         50         60 
MVQSKAKAGF QAGVKDYRLT YYTPDYTPKD TDLLACFRVT PQPGVPPEEA GAAVAAESST 

        70         80         90        100        110        120 
GTWTTVWTDG LTDLDRYKGR CYDIEPVPNE DNQYFCFVAY PLDLFEEGSV TNILTSIVGN 

       130        140        150        160        170        180 
VFGFKALRAL RLEDIRFPVA LLKTFQGPPH GITVERDKLN KYGRPLLGCT IKPKLGLSAK 

       190        200        210        220        230        240 
NYGRAVYECL RGGLDFTKDD ENINSQPFMR WRDRFLFVQE AIEKSQAETN EIKGHYLNVT 

       250        260        270        280        290        300 
AGTLEEMMKR AEFAKEIKTP IIMHDYLTGG FTANTTLARW CRDNGVLLHI HRAMHAVIDR 

       310        320        330        340        350        360 
QKNHGIHFRV LAKCLRMSGG DHLHSGTVVG KLEGERGITM GFVDLMREDY VEEDRARGIF 

       370        380        390        400        410        420 
FTQDWASMPG VMPVASGGIH VWHMPALVEI FGDDSCLQFG GGTLGHPWGN APGATANRVA 

       430        440        450        460        470 
LEACIQARNE GRSLAREGNE VIREAARWSP ELAAACELWK EIKFEFEAMD TL 

« Hide

References

[1]"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 7424.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001291 Genomic DNA. Translation: ACK69811.1.
RefSeqYP_002376679.1. NC_011729.1.

3D structure databases

ProteinModelPortalB7K7P3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING65393.PCC7424_1367.

Proteomic databases

PRIDEB7K7P3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK69811; ACK69811; PCC7424_1367.
GeneID7111266.
KEGGcyc:PCC7424_1367.
PATRIC21551552. VBICyaSp136448_1405.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1850.
HOGENOMHOG000230831.
KOK01601.
OMAHRAMHAA.
OrthoDBEOG6ZKXMS.
ProtClustDBPRK04208.

Enzyme and pathway databases

BioCycCSP65393:GJP7-1382-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01338. RuBisCO_L_type1.
InterProIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRBL_CYAP7
AccessionPrimary (citable) accession number: B7K7P3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families