Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

B7K5H5

- SYI_CYAP8

UniProt

B7K5H5 - SYI_CYAP8

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
    Binding sitei613 – 6131ATPUniRule annotation
    Metal bindingi928 – 9281ZincUniRule annotation
    Metal bindingi931 – 9311ZincUniRule annotation
    Metal bindingi948 – 9481ZincUniRule annotation
    Metal bindingi951 – 9511ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCSP41431:GHLK-2738-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PCC8801_2707
    OrganismiCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
    Taxonomic identifieri41431 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
    ProteomesiUP000008204: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 959959Isoleucine--tRNA ligasePRO_1000189148Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi41431.PCC8801_2707.

    Structurei

    3D structure databases

    ProteinModelPortaliB7K5H5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi60 – 7011"HIGH" regionAdd
    BLAST
    Motifi610 – 6145"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7K5H5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTEPKSYKDT VNLPQTDFSM RANAVQREPE IQQFWTENCI YEQLSQNNPE    50
    DLFILHDGPP YANGSLHMGH ALNKTLKDII NKYKLLRGHK VRYVPGWDCH 100
    GLPIELKVLQ SMKSEEREGL TPLKLRRKAR DFALKTQQEQ AEGFKRYGVW 150
    GDWENPYLTL TPEYEAAQIG VFGQMALKGY IYRGLKPVHW SPSSRTALAE 200
    AELEYPEGHT SQSIFAAFPI IKSSKDAQEI LDPFLSNLGV AIWTTTPWTL 250
    PGNLAVALNP ELTYAIVEQT SNLCNYQYII VAADLVERLS ATFSTELTVK 300
    ATLPGQILEH TIYRHPLYDR ESEIVIGGDY VTTESGTGLV HTAPGHGQED 350
    YIVGQRYGLQ VLSPVDDAGN FTEEAGQFSG LNVLKDANQA IINELKNKGS 400
    LLKEEPYLHK YPYDWRTKKP TIFRATEQWF ASVEGFREAA LEAIKSVNWI 450
    PPQGENRITP MVSDRSDWCI SRQRSWGVPI PVFYNEETNE PLLTEETINH 500
    VQAIIAKQGS DAWWELSIEE LLPEQYKKDA HKYRRGTDTM DVWFDSGSSW 550
    AAVAKQREEL KYPVDIYLEG SDQHRGWFQS SLLTSVAVNG IAPYKTVLTH 600
    GFVLDEKGHK MSKSLGNIVD PLVIINGGKN QKQEPPYGAD VLRLWVSSVD 650
    YSSDVPIGQT ILKQLSDVYR KIRNTARFLL GNLHDFDPEK DTVSYDQLPE 700
    LDQYMLHRIT EVFTEVTDAF EKFQFFRFFQ TVQNFCVVDL SNFYLDIAKD 750
    RLYISDTNSL RRRSCQTVLK VAVESLAKAI APVLCHMAED IWQFLPYKTP 800
    YQSVFASGWV EMQKQWERPE LTASWGKLRQ IRTEVNKVLE QARNEKAIGS 850
    SLDAKVLLYV SDQDFKKQLE SFNPNDSLKG NQVDELRYLV LASQVELVDS 900
    LEAIKKADYQ SESELVSVGV VKAEGQKCDR CWNYSTKVGE FSDDPTICER 950
    CNAALVGEF 959
    Length:959
    Mass (Da):109,180
    Last modified:February 10, 2009 - v1
    Checksum:i56C3E3F7EB87F669
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001287 Genomic DNA. Translation: ACK66708.1.
    RefSeqiWP_012595975.1. NC_011726.1.
    YP_002372864.1. NC_011726.1.

    Genome annotation databases

    EnsemblBacteriaiACK66708; ACK66708; PCC8801_2707.
    GeneIDi7102093.
    KEGGicyp:PCC8801_2707.
    PATRICi21577729. VBICyaSp125535_2880.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001287 Genomic DNA. Translation: ACK66708.1 .
    RefSeqi WP_012595975.1. NC_011726.1.
    YP_002372864.1. NC_011726.1.

    3D structure databases

    ProteinModelPortali B7K5H5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 41431.PCC8801_2707.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK66708 ; ACK66708 ; PCC8801_2707 .
    GeneIDi 7102093.
    KEGGi cyp:PCC8801_2707.
    PATRICi 21577729. VBICyaSp125535_2880.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CSP41431:GHLK-2738-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
      Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
      MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 8801.

    Entry informationi

    Entry nameiSYI_CYAP8
    AccessioniPrimary (citable) accession number: B7K5H5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3