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B7K5H5

- SYI_CYAP8

UniProt

B7K5H5 - SYI_CYAP8

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Protein

Isoleucine--tRNA ligase

Gene
ileS, PCC8801_2707
Organism
Cyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylate By similarity
Binding sitei613 – 6131ATP By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity
Metal bindingi948 – 9481Zinc By similarity
Metal bindingi951 – 9511Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCSP41431:GHLK-2738-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PCC8801_2707
OrganismiCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Taxonomic identifieri41431 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000008204: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Isoleucine--tRNA ligaseUniRule annotationPRO_1000189148Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi41431.PCC8801_2707.

Structurei

3D structure databases

ProteinModelPortaliB7K5H5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotationAdd
BLAST
Motifi610 – 6145"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7K5H5-1 [UniParc]FASTAAdd to Basket

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MTEPKSYKDT VNLPQTDFSM RANAVQREPE IQQFWTENCI YEQLSQNNPE    50
DLFILHDGPP YANGSLHMGH ALNKTLKDII NKYKLLRGHK VRYVPGWDCH 100
GLPIELKVLQ SMKSEEREGL TPLKLRRKAR DFALKTQQEQ AEGFKRYGVW 150
GDWENPYLTL TPEYEAAQIG VFGQMALKGY IYRGLKPVHW SPSSRTALAE 200
AELEYPEGHT SQSIFAAFPI IKSSKDAQEI LDPFLSNLGV AIWTTTPWTL 250
PGNLAVALNP ELTYAIVEQT SNLCNYQYII VAADLVERLS ATFSTELTVK 300
ATLPGQILEH TIYRHPLYDR ESEIVIGGDY VTTESGTGLV HTAPGHGQED 350
YIVGQRYGLQ VLSPVDDAGN FTEEAGQFSG LNVLKDANQA IINELKNKGS 400
LLKEEPYLHK YPYDWRTKKP TIFRATEQWF ASVEGFREAA LEAIKSVNWI 450
PPQGENRITP MVSDRSDWCI SRQRSWGVPI PVFYNEETNE PLLTEETINH 500
VQAIIAKQGS DAWWELSIEE LLPEQYKKDA HKYRRGTDTM DVWFDSGSSW 550
AAVAKQREEL KYPVDIYLEG SDQHRGWFQS SLLTSVAVNG IAPYKTVLTH 600
GFVLDEKGHK MSKSLGNIVD PLVIINGGKN QKQEPPYGAD VLRLWVSSVD 650
YSSDVPIGQT ILKQLSDVYR KIRNTARFLL GNLHDFDPEK DTVSYDQLPE 700
LDQYMLHRIT EVFTEVTDAF EKFQFFRFFQ TVQNFCVVDL SNFYLDIAKD 750
RLYISDTNSL RRRSCQTVLK VAVESLAKAI APVLCHMAED IWQFLPYKTP 800
YQSVFASGWV EMQKQWERPE LTASWGKLRQ IRTEVNKVLE QARNEKAIGS 850
SLDAKVLLYV SDQDFKKQLE SFNPNDSLKG NQVDELRYLV LASQVELVDS 900
LEAIKKADYQ SESELVSVGV VKAEGQKCDR CWNYSTKVGE FSDDPTICER 950
CNAALVGEF 959
Length:959
Mass (Da):109,180
Last modified:February 10, 2009 - v1
Checksum:i56C3E3F7EB87F669
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001287 Genomic DNA. Translation: ACK66708.1.
RefSeqiWP_012595975.1. NC_011726.1.
YP_002372864.1. NC_011726.1.

Genome annotation databases

EnsemblBacteriaiACK66708; ACK66708; PCC8801_2707.
GeneIDi7102093.
KEGGicyp:PCC8801_2707.
PATRICi21577729. VBICyaSp125535_2880.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001287 Genomic DNA. Translation: ACK66708.1 .
RefSeqi WP_012595975.1. NC_011726.1.
YP_002372864.1. NC_011726.1.

3D structure databases

ProteinModelPortali B7K5H5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 41431.PCC8801_2707.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK66708 ; ACK66708 ; PCC8801_2707 .
GeneIDi 7102093.
KEGGi cyp:PCC8801_2707.
PATRICi 21577729. VBICyaSp125535_2880.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CSP41431:GHLK-2738-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 8801.

Entry informationi

Entry nameiSYI_CYAP8
AccessioniPrimary (citable) accession number: B7K5H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: September 3, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi