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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Cyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei569Aminoacyl-adenylateUniRule annotation1
Binding sitei613ATPUniRule annotation1
Metal bindingi928ZincUniRule annotation1
Metal bindingi931ZincUniRule annotation1
Metal bindingi948ZincUniRule annotation1
Metal bindingi951ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PCC8801_2707
OrganismiCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Taxonomic identifieri41431 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeOscillatorialesCyanothecaceaeCyanothece
Proteomesi
  • UP000008204 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891481 – 959Isoleucine--tRNA ligaseAdd BLAST959

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi41431.PCC8801_2707

Structurei

3D structure databases

ProteinModelPortaliB7K5H5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi60 – 70"HIGH" regionAdd BLAST11
Motifi610 – 614"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B7K5H5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEPKSYKDT VNLPQTDFSM RANAVQREPE IQQFWTENCI YEQLSQNNPE
60 70 80 90 100
DLFILHDGPP YANGSLHMGH ALNKTLKDII NKYKLLRGHK VRYVPGWDCH
110 120 130 140 150
GLPIELKVLQ SMKSEEREGL TPLKLRRKAR DFALKTQQEQ AEGFKRYGVW
160 170 180 190 200
GDWENPYLTL TPEYEAAQIG VFGQMALKGY IYRGLKPVHW SPSSRTALAE
210 220 230 240 250
AELEYPEGHT SQSIFAAFPI IKSSKDAQEI LDPFLSNLGV AIWTTTPWTL
260 270 280 290 300
PGNLAVALNP ELTYAIVEQT SNLCNYQYII VAADLVERLS ATFSTELTVK
310 320 330 340 350
ATLPGQILEH TIYRHPLYDR ESEIVIGGDY VTTESGTGLV HTAPGHGQED
360 370 380 390 400
YIVGQRYGLQ VLSPVDDAGN FTEEAGQFSG LNVLKDANQA IINELKNKGS
410 420 430 440 450
LLKEEPYLHK YPYDWRTKKP TIFRATEQWF ASVEGFREAA LEAIKSVNWI
460 470 480 490 500
PPQGENRITP MVSDRSDWCI SRQRSWGVPI PVFYNEETNE PLLTEETINH
510 520 530 540 550
VQAIIAKQGS DAWWELSIEE LLPEQYKKDA HKYRRGTDTM DVWFDSGSSW
560 570 580 590 600
AAVAKQREEL KYPVDIYLEG SDQHRGWFQS SLLTSVAVNG IAPYKTVLTH
610 620 630 640 650
GFVLDEKGHK MSKSLGNIVD PLVIINGGKN QKQEPPYGAD VLRLWVSSVD
660 670 680 690 700
YSSDVPIGQT ILKQLSDVYR KIRNTARFLL GNLHDFDPEK DTVSYDQLPE
710 720 730 740 750
LDQYMLHRIT EVFTEVTDAF EKFQFFRFFQ TVQNFCVVDL SNFYLDIAKD
760 770 780 790 800
RLYISDTNSL RRRSCQTVLK VAVESLAKAI APVLCHMAED IWQFLPYKTP
810 820 830 840 850
YQSVFASGWV EMQKQWERPE LTASWGKLRQ IRTEVNKVLE QARNEKAIGS
860 870 880 890 900
SLDAKVLLYV SDQDFKKQLE SFNPNDSLKG NQVDELRYLV LASQVELVDS
910 920 930 940 950
LEAIKKADYQ SESELVSVGV VKAEGQKCDR CWNYSTKVGE FSDDPTICER

CNAALVGEF
Length:959
Mass (Da):109,180
Last modified:February 10, 2009 - v1
Checksum:i56C3E3F7EB87F669
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001287 Genomic DNA Translation: ACK66708.1
RefSeqiWP_012595975.1, NC_011726.1

Genome annotation databases

EnsemblBacteriaiACK66708; ACK66708; PCC8801_2707
KEGGicyp:PCC8801_2707

Similar proteinsi

Entry informationi

Entry nameiSYI_CYAP8
AccessioniPrimary (citable) accession number: B7K5H5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: March 28, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health