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Reviewed, UniProtKB/Swiss-Prot B7K3R6 (GLO2_CYAP8)

Last modified February 9, 2010. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Hydroxyacylglutathione hydrolase
    EC=3.1.2.6
Alternative name(s):
    Glyoxalase II
      Short name=Glx II
Gene names
Name: gloB
Ordered Locus Names: PCC8801_2445
OrganismCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1)) [Complete proteome] [HAMAP]
Taxonomic identifier41431 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Chroococcales › Cyanothece

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Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374

Catalytic activity

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374

Subunit structure

Monomer By similarity. HAMAP MF_01374

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

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Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionhydroxyacylglutathione hydrolase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Hydroxyacylglutathione hydrolase HAMAP MF_01374
PRO_1000144756

Sites

Metal binding541Zinc 1 By similarity
Metal binding561Zinc 1 By similarity
Metal binding581Zinc 2 By similarity
Metal binding591Zinc 2 By similarity
Metal binding1131Zinc 1 By similarity
Metal binding1371Zinc 1 By similarity
Metal binding1371Zinc 2 By similarity
Metal binding1751Zinc 2 By similarity

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Sequences

Sequence LengthMass (Da)Tools
B7K3R6-1 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 42A7FA79F2FE45EF

FASTA25728,982
        10         20         30         40         50         60 
MEIKRLPALS DNYIFLLYDA DTQTAAVVDP AEPTPVLECL NKLGAQLVAI FNTHHHYDHV 

        70         80         90        100        110        120 
GANNQLQQYF PNLCIYGGSE DRGRIPGQQV FLKEGDRVEF GQRVGEVLFV PGHTRAHIAY 

       130        140        150        160        170        180 
YFPPKLSQET GELFCGDTLF GGGCGRLFEG TPTQMVNSLT KLRNLPDNTR VWCAHEYTLK 

       190        200        210        220        230        240 
NLQFALTVDP DNLVLKNRYH QVKEFRHQGQ ATVPSILGEE KLTNPFLRWD NSAIQLTIGM 

       250 
TDPARVFGKL RGMKDTF

« Hide

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References

[1]"Complete sequence of chromosome of Cyanothece sp. PCC 8801."
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Brettin T., Detter J.C., Han C., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N. expand/collapse author list , Liberton M., Stoeckel J., Banerjee A., Singh A., Page L., Sato H., Zhao L., Sherman L., Pakrasi H.B., Richardson P.
Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001287 Genomic DNA. Translation: ACK66456.1.
RefSeqYP_002372612.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID7101588.
GenomeReviewsGene locus PCC8801_2445 in contig CP001287_GR.
KEGGcyp:PCC8801_2445.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG753931.

Family and domain databases

HAMAPMF_01374. Glyoxalase_2.
[Tree]
InterProIPR001279. Blactmase-like.
IPR017782. Hydroxyacylglutathione_Hdrlase.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
TIGRFAMsTIGR03413. GSH_gloB. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameGLO2_CYAP8
AccessionPrimary (citable) accession number: B7K3R6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: February 9, 2010
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents