B7K3N8 (PUR9_CYAP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
   EC=2.1.2.3
   Alternative name(s):
   AICAR transformylase
2. IMP cyclohydrolase
   EC=3.5.4.10
   Alternative name(s):
   ATIC
   IMP synthase
   Inosinicase

Gene names

Name:	purH
Ordered Locus Names:	PCC8801_1316

Organism

Cyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1)) [Complete proteome] [HAMAP]

Taxonomic identifier

41431 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Cyanothece

Protein attributes

Sequence length	516 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139 IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139 Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.
Domain	The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139
Sequence similarities	Belongs to the PurH family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Hydrolase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	IMP cyclohydrolase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylaminoimidazolecarboxamide formyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 516	516	Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139		PRO_1000117869

Sequences

	Sequence		Length	Mass (Da)
	B7K3N8 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 10AC3378788CFE1B	FASTA	516	55,482
	10 20 30 40 50 60 MTRLALLSVS DKTGIIDLAQ QLIHQFDFEL ISSGGTAKAL QAAGLPVTKV SEYTGSPEIL 70 80 90 100 110 120 GGRVKTLHPR IHGGILGRRD LPQDTQDMET HQIHPIDLVV VNLYPFEQTI ANPNVTVAEA 130 140 150 160 170 180 IENIDIGGPT LLRAAAKNYA HVTVLSNPKY YDTYLQELAE NNGEVSLEFR QKMAGETFAL 190 200 210 220 230 240 TNSYDGAIAN YFMTLSSENE STLPSRFTVS GTQFQSLRYG ENPHQQAAWY QTGTQPSGWA 250 260 270 280 290 300 AATQLQGKEL SYNNLVDLEA ARRIIAEFNP QEPAVAILKH TNPCGVAVGN TLADAYEKAF 310 320 330 340 350 360 NADSISAFGG IIALNQPLDK ETASLLTKTF LECVVAPGCD DEAKEILTAK SKVRVLVLPD 370 380 390 400 410 420 LMNGPKQTIK VIAGGLLVQA SDDVIDTPDS WKIVTEKQPT LQQLAELLFA WKVAKHVKSN 430 440 450 460 470 480 AIVVTKNRTT LGIGAGQMNR VGSVKIALEQ AGEAAMGGCL ASDGFFPFDD SVRTAAAAGI 490 500 510 KVIVQPGGSV KDKDSIAAAN ELGLVMMLTG IRHFLH

References

[1]

"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria." Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B. MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 8801.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001287 Genomic DNA. Translation: ACK65380.1.
RefSeq	YP_002371536.1. NC_011726.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	41431.PCC8801_1316.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACK65380; ACK65380; PCC8801_1316.
GeneID	7105059.
KEGG	cyp:PCC8801_1316.
PATRIC	21574871. VBICyaSp125535_1469.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0138.
HOGENOM	HOG000230372.
KO	K00602.
OMA	DLLFAWK.
ProtClustDB	PRK00881.
Enzyme and pathway databases
BioCyc	CSP41431:GHLK-1338-MONOMER.
UniPathway	UPA00074; UER00133. UPA00074; UER00135.
Family and domain databases
Gene3D	3.40.140.20. 2 hits. 3.40.50.1380. 1 hit.
HAMAP	MF_00139. PurH.
InterPro	IPR024051. AICAR_Tfase_dom. IPR002695. AICARFT_IMPCHas. IPR016193. Cytidine_deaminase-like. IPR011607. MGS-like_dom. [Graphical view]
PANTHER	PTHR11692. PTHR11692. 1 hit.
Pfam	PF01808. AICARFT_IMPCHas. 1 hit. PF02142. MGS. 1 hit. [Graphical view]
PIRSF	PIRSF000414. AICARFT_IMPCHas. 1 hit.
SMART	SM00798. AICARFT_IMPCHas. 1 hit. SM00851. MGS. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit. SSF52335. MGS-like_dom. 1 hit.
TIGRFAMs	TIGR00355. purH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR9_CYAP8

Accession

Primary (citable) accession number: B7K3N8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program