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B7K1U3

- BIOB_CYAP8

UniProt

B7K1U3 - BIOB_CYAP8

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Protein
Biotin synthase
Gene
bioB, PCC8801_0144
Organism
Cyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi96 – 961Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi137 – 1371Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi174 – 1741Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi234 – 2341Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi304 – 3041Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSP41431:GHLK-144-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:PCC8801_0144
OrganismiCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Taxonomic identifieri41431 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000008204: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 368368Biotin synthaseUniRule annotation
PRO_0000381340Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi41431.PCC8801_0144.

Structurei

3D structure databases

ProteinModelPortaliB7K1U3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B7K1U3-1 [UniParc]FASTAAdd to Basket

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MVQAPLSPSL LQTQSISQPP QETEALKAWL EELTQKIIEG DRINKSEALT    50
LTQIEGQDSI LLLCEAADRI RQACCGNVVD LCSIINIKSG NCSENCRFCS 100
QSVYHPGENS PIYGLKSSEE ILAQAKAAEA AGAKRFCLVS QGRGPKYQGA 150
KSKEFEQILA TVRQIAAETS IKPCCALGEV TPEQAQALRE AGVTRYNHNL 200
EASEGFYPEI VTSHSWRDRV ETIKNLKAAG IQACSGGIMG MGETWEDRVD 250
LALALRELGV ESVPINLLNP REGTPLGDCH RLDPFEALKA IAIFRLILPQ 300
QILRYAGGRE AIMGDLQSLG LKSGINAMLI GHYLTTLGQP PEKDLAMVES 350
LGLQGGEAPI PGEYQTRS 368
Length:368
Mass (Da):39,902
Last modified:February 10, 2009 - v1
Checksum:i8C58830EC4590781
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001287 Genomic DNA. Translation: ACK64250.1.
RefSeqiWP_012593527.1. NC_011726.1.
YP_002370406.1. NC_011726.1.

Genome annotation databases

EnsemblBacteriaiACK64250; ACK64250; PCC8801_0144.
GeneIDi7104730.
KEGGicyp:PCC8801_0144.
PATRICi21572433. VBICyaSp125535_0260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001287 Genomic DNA. Translation: ACK64250.1 .
RefSeqi WP_012593527.1. NC_011726.1.
YP_002370406.1. NC_011726.1.

3D structure databases

ProteinModelPortali B7K1U3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 41431.PCC8801_0144.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK64250 ; ACK64250 ; PCC8801_0144 .
GeneIDi 7104730.
KEGGi cyp:PCC8801_0144.
PATRICi 21572433. VBICyaSp125535_0260.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CSP41431:GHLK-144-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 8801.

Entry informationi

Entry nameiBIOB_CYAP8
AccessioniPrimary (citable) accession number: B7K1U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 10, 2009
Last modified: September 3, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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