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B7K1E7

- DAPF_CYAP8

UniProt

B7K1E7 - DAPF_CYAP8

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Cyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (10 Feb 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131SubstrateUniRule annotation
    Binding sitei47 – 471SubstrateUniRule annotation
    Binding sitei66 – 661SubstrateUniRule annotation
    Active sitei75 – 751Proton donor/acceptorUniRule annotation
    Binding sitei164 – 1641SubstrateUniRule annotation
    Sitei166 – 1661Important for catalytic activityUniRule annotation
    Binding sitei197 – 1971SubstrateUniRule annotation
    Sitei215 – 2151Important for catalytic activityUniRule annotation
    Active sitei224 – 2241Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciCSP41431:GHLK-2345-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:PCC8801_2326
    OrganismiCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
    Taxonomic identifieri41431 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
    ProteomesiUP000008204: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 281281Diaminopimelate epimerasePRO_1000118666Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi75 ↔ 224

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi41431.PCC8801_2326.

    Structurei

    3D structure databases

    ProteinModelPortaliB7K1E7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni10 – 112Substrate bindingUniRule annotation
    Regioni75 – 773Substrate bindingUniRule annotation
    Regioni215 – 2162Substrate bindingUniRule annotation
    Regioni225 – 2262Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0253.
    HOGENOMiHOG000220466.
    KOiK01778.
    OMAiLIVEPPY.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7K1E7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIEFSKYQG LGNDFILIDN RSSHTPLVSP EQAIKMCDRH FGIGADGVIF    50
    VLPGTANTDY TMRIFNSDGS EPQMCGNGIR CLARFIAHLE GGETLGKTYQ 100
    IDTLAGVISP RLEAQGQVKV DMGTPYLLAK EIPTTLGNSN DKVINHSLEV 150
    AGQTWLVTCV SMGNPHCITF VKDVWGIDLP TLGPQFEHHP GFPERTNTEF 200
    IEVVRRDYVK MRVWERGAGI TLACGTGACA SVVAGVLTNQ CDRYCIVELP 250
    GGNLTIEWSE MDNRIYMTGP AELVFTGIYN L 281
    Length:281
    Mass (Da):30,810
    Last modified:February 10, 2009 - v1
    Checksum:iACFB45BD1832FCDB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001287 Genomic DNA. Translation: ACK66342.1.
    RefSeqiWP_012595610.1. NC_011726.1.
    YP_002372498.1. NC_011726.1.

    Genome annotation databases

    EnsemblBacteriaiACK66342; ACK66342; PCC8801_2326.
    GeneIDi7103245.
    KEGGicyp:PCC8801_2326.
    PATRICi21576933. VBICyaSp125535_2492.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001287 Genomic DNA. Translation: ACK66342.1 .
    RefSeqi WP_012595610.1. NC_011726.1.
    YP_002372498.1. NC_011726.1.

    3D structure databases

    ProteinModelPortali B7K1E7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 41431.PCC8801_2326.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK66342 ; ACK66342 ; PCC8801_2326 .
    GeneIDi 7103245.
    KEGGi cyp:PCC8801_2326.
    PATRICi 21576933. VBICyaSp125535_2492.

    Phylogenomic databases

    eggNOGi COG0253.
    HOGENOMi HOG000220466.
    KOi K01778.
    OMAi LIVEPPY.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci CSP41431:GHLK-2345-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
      Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
      MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PCC 8801.

    Entry informationi

    Entry nameiDAPF_CYAP8
    AccessioniPrimary (citable) accession number: B7K1E7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: February 10, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3