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B7K0C3

- B7K0C3_CYAP8

UniProt

B7K0C3 - B7K0C3_CYAP8

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Protein

Riboflavin biosynthesis protein RibBA

Gene

ribBA

Organism
Cyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Status
Unreviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotation
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.UniRule annotation

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation
GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate.UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation
Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi36 – 361Magnesium or manganese 1UniRule annotation
Metal bindingi36 – 361Magnesium or manganese 2UniRule annotation
Binding sitei40 – 401D-ribulose 5-phosphateUniRule annotation
Sitei136 – 1361Essential for DHBP synthase activityUniRule annotation
Metal bindingi153 – 1531Magnesium or manganese 2UniRule annotation
Binding sitei174 – 1741D-ribulose 5-phosphateUniRule annotation
Sitei174 – 1741Essential for DHBP synthase activityUniRule annotation
Metal bindingi269 – 2691Zinc; catalyticUniRule annotation
Metal bindingi280 – 2801Zinc; catalyticUniRule annotation
Metal bindingi282 – 2821Zinc; catalyticUniRule annotation
Binding sitei285 – 2851GTPUniRule annotation
Binding sitei329 – 3291GTPUniRule annotation
Active sitei341 – 3411Proton acceptor; for GTP cyclohydrolase activityUniRule annotation
Active sitei343 – 3431Nucleophile; for GTP cyclohydrolase activityUniRule annotation
Binding sitei364 – 3641GTPUniRule annotation
Binding sitei369 – 3691GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi264 – 2685GTPUniRule annotation
Nucleotide bindingi307 – 3093GTPUniRule annotation

GO - Molecular functioni

  1. 3,4-dihydroxy-2-butanone-4-phosphate synthase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-KW
  3. GTP cyclohydrolase II activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
  6. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. riboflavin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationImported, LyaseUniRule annotation

Keywords - Biological processi

Riboflavin biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

GTP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding, ZincUniRule annotation

Enzyme and pathway databases

BioCyciCSP41431:GHLK-3480-MONOMER.
UniPathwayiUPA00275; UER00399.
UPA00275; UER00400.

Names & Taxonomyi

Protein namesi
Recommended name:
Riboflavin biosynthesis protein RibBAUniRule annotation
Gene namesi
Name:ribBAUniRule annotation
Ordered Locus Names:PCC8801_3439Imported
OrganismiCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))Imported
Taxonomic identifieri41431 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000008204: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi41431.PCC8801_3439.

Structurei

3D structure databases

ProteinModelPortaliB7K0C3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 211211DHBP synthaseUniRule annotationAdd
BLAST
Regioni35 – 362D-ribulose 5-phosphate bindingUniRule annotation
Regioni150 – 1545D-ribulose 5-phosphate bindingUniRule annotation
Regioni212 – 556345GTP cyclohydrolase IIUniRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the GTP cyclohydrolase II family.UniRule annotation
In the N-terminal section; belongs to the DHBP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0108.
HOGENOMiHOG000115440.
KOiK14652.
OMAiGKGLICM.
OrthoDBiEOG679TK8.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
HAMAPiMF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProiIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
PfamiPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.

Sequencei

Sequence statusi: Complete.

B7K0C3 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDASPNAIAQ FDTIDAALAD IKAGKAIIVV DDENRENEGD LICAAQFATP
60 70 80 90 100
NMINFMAVEA RGLICLAMMG ERLDTLDLPL MVTKNTDSNQ TAFTVSIDAA
110 120 130 140 150
KHLGVSTGIS AEDRARTIQV AINPDTHPDD LTRPGHIFPI RAKEGGVLKR
160 170 180 190 200
AGHTEAAVDL SRLAGLYPAG VICEIQNPDG SMARLPELFE YAKKHELKLI
210 220 230 240 250
SIADLISYRL KHDRFVYRET VCQFPSQFGT FQLYAYRNVL DGTEHVAIVK
260 270 280 290 300
GDPAQFKDQP VMVRMHSECL TGDALGSMRC DCRMQLQTAL KMIEGSGLGV
310 320 330 340 350
VVYLRQEGRG IGLVNKLKAY SLQDMGLDTV EANERLGFPA DLRDYGMGAQ
360 370 380 390 400
MLNDLGVKQI RLITNNPRKI AGLKGYGLEV VDRVPLLIEA NDYNANYLAT
410 420 430 440 450
KAEKLGHLLL HTYLITVAID WETEMRSAKE RYGNLEKLRQ LCRSSQLLLQ
460 470 480 490 500
EEVRPIANAL FSSPSLIFHL GFEQGKMVDP HWYHNSKHPY LSAIAQILDE
510 520 530 540 550
IVTWPNIKRL EFLISSGDDP LLGLQVQLDR HTFSLKDQPS EYLRELEMQT

IYSFQG
Length:556
Mass (Da):61,992
Last modified:February 10, 2009 - v1
Checksum:iABDD8DBF8EF3DE98
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001287 Genomic DNA. Translation: ACK67407.1.
RefSeqiYP_002373563.1. NC_011726.1.

Genome annotation databases

EnsemblBacteriaiACK67407; ACK67407; PCC8801_3439.
GeneIDi7103129.
KEGGicyp:PCC8801_3439.
PATRICi21579233. VBICyaSp125535_3621.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001287 Genomic DNA. Translation: ACK67407.1 .
RefSeqi YP_002373563.1. NC_011726.1.

3D structure databases

ProteinModelPortali B7K0C3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 41431.PCC8801_3439.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK67407 ; ACK67407 ; PCC8801_3439 .
GeneIDi 7103129.
KEGGi cyp:PCC8801_3439.
PATRICi 21579233. VBICyaSp125535_3621.

Phylogenomic databases

eggNOGi COG0108.
HOGENOMi HOG000115440.
KOi K14652.
OMAi GKGLICM.
OrthoDBi EOG679TK8.

Enzyme and pathway databases

UniPathwayi UPA00275 ; UER00399 .
UPA00275 ; UER00400 .
BioCyci CSP41431:GHLK-3480-MONOMER.

Family and domain databases

Gene3Di 3.90.870.10. 1 hit.
HAMAPi MF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProi IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view ]
Pfami PF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view ]
SUPFAMi SSF55821. SSF55821. 1 hit.
TIGRFAMsi TIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 8801Imported.

Entry informationi

Entry nameiB7K0C3_CYAP8
AccessioniPrimary (citable) accession number: B7K0C3
Entry historyi
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotation, Reference proteomeImported

External Data

Dasty 3