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B7JZB2 (DDL_CYAP8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:PCC8801_3354
OrganismCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1)) [Complete proteome] [HAMAP]
Taxonomic identifier41431 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 351351D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000116637

Regions

Domain135 – 344210ATP-grasp
Nucleotide binding171 – 22656ATP By similarity

Sites

Metal binding2971Magnesium or manganese 1 By similarity
Metal binding3111Magnesium or manganese 1 By similarity
Metal binding3111Magnesium or manganese 2 By similarity
Metal binding3131Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B7JZB2 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: F2CCE6EFCCF84363

FASTA35138,458
        10         20         30         40         50         60 
MAKLRVGLLF GGRSGEHEVS LNSAKAIATA LQSGENPQKY DIIPIYIQKN GVWQAGDTAQ 

        70         80         90        100        110        120 
QVLNDAKPLP CEDATSQQLW TFPPQVSEVD VWFPILHGPN GEDGTLQGLL TLMQTPFVGS 

       130        140        150        160        170        180 
GVLGSAVGMD KIAMKMAFAQ AGLPQVKYIA VDRAQIWSNP CVFPKLCDEI EQRLGYPCFV 

       190        200        210        220        230        240 
KPANLGSSVG IAKVRSRSEL EKALDSAASY DRRIVIETGV KAREVECAVL GNENPKASVI 

       250        260        270        280        290        300 
GEITYNSDFY DYEAKYTDGM AQMHIPAQLP DAIVTQIQDM AIQAFKAVDG AGLARVDFFY 

       310        320        330        340        350 
VEDSQEVFIN EINTLPGFTA FSMYPQLWKE TGVPFAELVD QLIQLALERK Q 

« Hide

References

[1]"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 8801.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001287 Genomic DNA. Translation: ACK67323.1.
RefSeqYP_002373479.1. NC_011726.1.

3D structure databases

ProteinModelPortalB7JZB2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING41431.PCC8801_3354.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK67323; ACK67323; PCC8801_3354.
GeneID7103010.
KEGGcyp:PCC8801_3354.
PATRIC21579063. VBICyaSp125535_3537.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycCSP41431:GHLK-3393-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CYAP8
AccessionPrimary (citable) accession number: B7JZB2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: February 19, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways