Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Cyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotationSAAS annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotationSAAS annotation

Cofactori

FADUniRule annotationSAAS annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 186FADUniRule annotation

GO - Molecular functioni

  1. 5,10-methylenetetrahydrofolate-dependent tRNA (m5U54) methyltransferase activity Source: UniProtKB-HAMAP
  2. flavin adenine dinucleotide binding Source: UniProtKB-HAMAP
  3. methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

MethyltransferaseUniRule annotationSAAS annotation, Transferase

Keywords - Biological processi

tRNA processingUniRule annotationSAAS annotation

Keywords - Ligandi

FADUniRule annotationSAAS annotation, Flavoprotein

Enzyme and pathway databases

BioCyciCSP41431:GHLK-812-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotationSAAS annotation (EC:2.1.1.74UniRule annotationSAAS annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:PCC8801_0806Imported
OrganismiCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))Imported
Taxonomic identifieri41431 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000008204 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Protein-protein interaction databases

STRINGi41431.PCC8801_0806.

Structurei

3D structure databases

ProteinModelPortaliB7JYL8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.
OrthoDBiEOG6J74VT.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7JYL8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDRSTTTIQ VIGGGLAGTE AAWQIAQAGV PVILYEMRPL RTSPAHHSEE
60 70 80 90 100
LAELVCSNSF GAMSSDRAAG LLHEELRRLG SIIIQTADQH SVPAGGALAV
110 120 130 140 150
DRGVFSHALT QTLDNHPLIT LKRAAIGQIP SEGIVVLTTG PLTTPDLAED
160 170 180 190 200
LQRFTGMDYL SFFDAASPII VGDSINRDIA FLASRYDKGE AAYLNCPLTK
210 220 230 240 250
EQYLHFREEL CQAEQAELKD FERETAQFFE GCLPIEELAK RGEDTMRYGP
260 270 280 290 300
LKPVGLFDSR LGDFRDPENK GKRPYGVVQL RQEDKNGQLW NMVGFQTNLK
310 320 330 340 350
WGEQKRIFRL IPGLETAEFV RMGVMHRNTF INSPQLLEAT LQFKSRPTLL
360 370 380 390 400
AAGQLIGTEG YSAAAAGGWL AGTNAARLAL RLDPLKMPET TMMGALFEFI
410 420 430 440 450
SSASPKHFQP MPPNFGIIPE LSHKIGNKRE RYGKYRDRAL ADLETWNNQS

VNKRSLCVQ
Length:459
Mass (Da):50,805
Last modified:February 10, 2009 - v1
Checksum:i072BE7030E92E378
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001287 Genomic DNA. Translation: ACK64888.1.
RefSeqiYP_002371044.1. NC_011726.1.

Genome annotation databases

EnsemblBacteriaiACK64888; ACK64888; PCC8801_0806.
KEGGicyp:PCC8801_0806.
PATRICi21573822. VBICyaSp125535_0949.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001287 Genomic DNA. Translation: ACK64888.1.
RefSeqiYP_002371044.1. NC_011726.1.

3D structure databases

ProteinModelPortaliB7JYL8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi41431.PCC8801_0806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK64888; ACK64888; PCC8801_0806.
KEGGicyp:PCC8801_0806.
PATRICi21573822. VBICyaSp125535_0949.

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciCSP41431:GHLK-812-MONOMER.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 8801Imported.

Entry informationi

Entry nameiB7JYL8_CYAP8
AccessioniPrimary (citable) accession number: B7JYL8
Entry historyi
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: April 1, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.