Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B7JYA9 (SYL_CYAP8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PCC8801_3236
OrganismCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1)) [Complete proteome] [HAMAP]
Taxonomic identifier41431 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece

Protein attributes

Sequence length856 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 856856Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199191

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif617 – 6215"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6201ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B7JYA9 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: C5B2469C542BE1FE

FASTA85697,318
        10         20         30         40         50         60 
MESQYNAAAI EQKWQQDWVK QGLDKTPENS NKPKFYALSM FPYPSGNLHM GHVRNYVITD 

        70         80         90        100        110        120 
VIARLKRLQG YRVLHPMGWD AFGLPAENAA IDRGIPPAEW TYKNMSQMKQ QLQTLGLSID 

       130        140        150        160        170        180 
WEREVATCAP EYYKWTQWLF LQLYQAGLAY QKEAAVNWDP IDQTVLANEQ VDSEGYSWRS 

       190        200        210        220        230        240 
GAKVERKLLR QWFLKITDYA EQLLTDLDKL PGWPDRVKLM QENWIGKSVG AYLEFPIKGS 

       250        260        270        280        290        300 
EEKIAVFTTR PDTVYGVTYV VLAPEHPLTL KVTTPEQKAA VDAFIEEVSN ESEIERTAED 

       310        320        330        340        350        360 
KPKRGILTGG TAINPFNGQE IPILIADYVL YEYGTGAVMG VPAHDTRDFK FAKEKQLPIK 

       370        380        390        400        410        420 
VVIIPENSDN TNPPLTEAYT DPGIMVNSGI FDGIQSTEGK TAIIEYAEKE GYGKARIQYR 

       430        440        450        460        470        480 
LRDWLISRQR YWGCPIPVVH CPSCGTVAVP DADLPVKLPE NVEFTGRDAS PLAKLEDWIN 

       490        500        510        520        530        540 
VPCPSCGEPA KRETDTMDTF IDSSWYYLRY TDANNNQEAF NLEKANDWMA IDQYVGGIEH 

       550        560        570        580        590        600 
AILHLLYSRF FTKALRDRGL VNIDEPFKRL LTQGMVQGLT YKNPQTGKYV PSENVIFQDD 

       610        620        630        640        650        660 
HYRDKETGEI LSFFYEKMSK SKYNGVDPKQ VLGKYGADTA RMFILFKAPP EKDLEWDDAD 

       670        680        690        700        710        720 
VEGQFRFLNR VWRLVANYLE NKPKTVKKAS ELTKEDKDLR RAIHTAIKEI SEDLEGDYQF 

       730        740        750        760        770        780 
NTAVSELMKL SNALTDAKCL ESGVYQEGIE TLLILLAPFA PHIAEELWHN LGYEKSIHLQ 

       790        800        810        820        830        840 
SWPTVNPDAL VVDEITLVIQ IMGKTRGTIQ VSATATKDEL EKLARESEVG QRYLDGQDVK 

       850 
KVIVVPGKLV NFVIAK 

« Hide

References

[1]"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 8801.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001287 Genomic DNA. Translation: ACK67211.1.
RefSeqYP_002373367.1. NC_011726.1.

3D structure databases

ProteinModelPortalB7JYA9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING41431.PCC8801_3236.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK67211; ACK67211; PCC8801_3236.
GeneID7105365.
KEGGcyp:PCC8801_3236.
PATRIC21578831. VBICyaSp125535_3421.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycCSP41431:GHLK-3275-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CYAP8
AccessionPrimary (citable) accession number: B7JYA9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries