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B7JVW5 (B7JVW5_CYAP8) Unreviewed, UniProtKB/TrEMBL

Last modified May 29, 2013. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase large chain HAMAP-Rule MF_01338
Short name=RuBisCO large subunit HAMAP-Rule MF_01338
EC=4.1.1.39 HAMAP-Rule MF_01338
Gene names
Name:cbbL HAMAP-Rule MF_01338
Synonyms:rbcL HAMAP-Rule MF_01338
Ordered Locus Names:PCC8801_1602 EMBL ACK65654.1
OrganismCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1)) [Complete proteome] [HAMAP] EMBL ACK65654.1
Taxonomic identifier41431 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Oscillatoriophycideae › Chroococcales › Cyanothece

Protein attributes

Sequence length472 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01338

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers By similarity. HAMAP-Rule MF_01338

Post-translational modification

The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover By similarity. HAMAP-Rule MF_01338

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity. HAMAP-Rule MF_01338

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily. HAMAP-Rule MF_01338

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1721Proton acceptor By similarity HAMAP-Rule MF_01338
Active site2911Proton acceptor By similarity HAMAP-Rule MF_01338
Metal binding1981Magnesium; via carbamate group By similarity HAMAP-Rule MF_01338
Metal binding2001Magnesium By similarity HAMAP-Rule MF_01338
Metal binding2011Magnesium By similarity HAMAP-Rule MF_01338
Binding site1201Substrate; in homodimeric partner By similarity HAMAP-Rule MF_01338
Binding site1701Substrate By similarity HAMAP-Rule MF_01338
Binding site1741Substrate By similarity HAMAP-Rule MF_01338
Binding site2921Substrate By similarity HAMAP-Rule MF_01338
Binding site3241Substrate By similarity HAMAP-Rule MF_01338
Binding site3761Substrate By similarity HAMAP-Rule MF_01338
Site3311Transition state stabilizer By similarity HAMAP-Rule MF_01338

Amino acid modifications

Modified residue1981N6-carboxylysine By similarity HAMAP-Rule MF_01338
Disulfide bond244Interchain; in linked form By similarity HAMAP-Rule MF_01338

Sequences

Sequence LengthMass (Da)Tools
B7JVW5 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 9E7709AB3A482831

FASTA47252,735
        10         20         30         40         50         60 
MVQAKSKSGF EAGVKDYRLT YYTPDYTPKD TDLLACFRMT PQPGVPPEEA GAAVAAESST 

        70         80         90        100        110        120 
GTWTTVWTDN LTDLDRYKGR CYDIEKVENE DNQYFCFIAY PLDLFEEGSV TNVLTSLVGN 

       130        140        150        160        170        180 
VFGFKALRAL RLEDIRFPVA LIKTFQGPPH GITVERDKLN KYGRPLLGCT IKPKLGLSAK 

       190        200        210        220        230        240 
NYGRAVYECL RGGLDFTKDD ENINSQPFMR WRDRFLFVQD AIEKAQAETN EVKGHYLNVT 

       250        260        270        280        290        300 
AGTCEEMLKR AEFAKEIKTP IIMHDFLTGG FTANTTLAKF CRDNGLLLHI HRAMHAVIDR 

       310        320        330        340        350        360 
QKNHGIHFRV LAKCLRLSGG DHLHSGTVVG KLEGERGITM GFVDLMREDY VEEDRSRGIF 

       370        380        390        400        410        420 
FTQDYASMPG TMPVASGGIH VWHMPALVEI FGDDSCLQFG GGTLGHPWGN APGATANRVA 

       430        440        450        460        470 
LEACIQARNE GRSLAREGND VIREACKWSP ELAAACELWK EIKFEFEAMD TL

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References

[1]"Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 8801.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001287 Genomic DNA. Translation: ACK65654.1.
RefSeqYP_002371810.1. NC_011726.1.

3D structure databases

ProteinModelPortalB7JVW5.
SMRB7JVW5. Positions 9-466.
ModBaseSearch...

Protein-protein interaction databases

STRING41431.PCC8801_1602.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK65654; ACK65654; PCC8801_1602.
GeneID7105640.
KEGGcyp:PCC8801_1602.
PATRIC21575449. VBICyaSp125535_1757.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1850.
HOGENOMHOG000230831.
KOK01601.
OMAFTQDWAS.
ProtClustDBPRK04208.

Enzyme and pathway databases

BioCycCSP41431:GHLK-1613-MONOMER.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01338. RuBisCO_L_type1.
InterProIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. RuBisCO_large. 1 hit.
SSF54966. RuBisCO_large. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB7JVW5_CYAP8
AccessionPrimary (citable) accession number: B7JVW5
Entry history
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: May 29, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info