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Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Cyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei120 – 1201Substrate; in homodimeric partnerUniRule annotation
Binding sitei170 – 1701SubstrateUniRule annotation
Active sitei172 – 1721Proton acceptorUniRule annotation
Binding sitei174 – 1741SubstrateUniRule annotation
Metal bindingi198 – 1981Magnesium; via carbamate groupUniRule annotation
Metal bindingi200 – 2001MagnesiumUniRule annotation
Metal bindingi201 – 2011MagnesiumUniRule annotation
Active sitei291 – 2911Proton acceptorUniRule annotation
Binding sitei292 – 2921SubstrateUniRule annotation
Binding sitei324 – 3241SubstrateUniRule annotation
Sitei331 – 3311Transition state stabilizerUniRule annotation
Binding sitei376 – 3761SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported, MonooxygenaseUniRule annotation, Oxidoreductase

Keywords - Biological processi

Calvin cycleUniRule annotation, Carbon dioxide fixationUniRule annotation, PhotorespirationUniRule annotation, PhotosynthesisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciCSP41431:GHLK-1613-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:cbbLUniRule annotation
Synonyms:rbcLUniRule annotation
Ordered Locus Names:PCC8801_1602Imported
OrganismiCyanothece sp. (strain PCC 8801) (Synechococcus sp. (strain PCC 8801 / RF-1))Imported
Taxonomic identifieri41431 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanothece
ProteomesiUP000008204 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei198 – 1981N6-carboxylysineUniRule annotation
Disulfide bondi244 – 244Interchain; in linked formUniRule annotation

Post-translational modificationi

The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover.UniRule annotation

Keywords - PTMi

Disulfide bondUniRule annotation

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers.UniRule annotation

Protein-protein interaction databases

STRINGi41431.PCC8801_1602.

Structurei

3D structure databases

ProteinModelPortaliB7JVW5.
SMRiB7JVW5. Positions 9-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1850.
HOGENOMiHOG000230831.
KOiK01601.
OMAiHAAFTRN.
OrthoDBiEOG6ZKXMS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7JVW5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQAKSKSGF EAGVKDYRLT YYTPDYTPKD TDLLACFRMT PQPGVPPEEA
60 70 80 90 100
GAAVAAESST GTWTTVWTDN LTDLDRYKGR CYDIEKVENE DNQYFCFIAY
110 120 130 140 150
PLDLFEEGSV TNVLTSLVGN VFGFKALRAL RLEDIRFPVA LIKTFQGPPH
160 170 180 190 200
GITVERDKLN KYGRPLLGCT IKPKLGLSAK NYGRAVYECL RGGLDFTKDD
210 220 230 240 250
ENINSQPFMR WRDRFLFVQD AIEKAQAETN EVKGHYLNVT AGTCEEMLKR
260 270 280 290 300
AEFAKEIKTP IIMHDFLTGG FTANTTLAKF CRDNGLLLHI HRAMHAVIDR
310 320 330 340 350
QKNHGIHFRV LAKCLRLSGG DHLHSGTVVG KLEGERGITM GFVDLMREDY
360 370 380 390 400
VEEDRSRGIF FTQDYASMPG TMPVASGGIH VWHMPALVEI FGDDSCLQFG
410 420 430 440 450
GGTLGHPWGN APGATANRVA LEACIQARNE GRSLAREGND VIREACKWSP
460 470
ELAAACELWK EIKFEFEAMD TL
Length:472
Mass (Da):52,735
Last modified:February 10, 2009 - v1
Checksum:i9E7709AB3A482831
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001287 Genomic DNA. Translation: ACK65654.1.
RefSeqiWP_012594927.1. NC_011726.1.
YP_002371810.1. NC_011726.1.

Genome annotation databases

EnsemblBacteriaiACK65654; ACK65654; PCC8801_1602.
KEGGicyp:PCC8801_1602.
PATRICi21575449. VBICyaSp125535_1757.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001287 Genomic DNA. Translation: ACK65654.1.
RefSeqiWP_012594927.1. NC_011726.1.
YP_002371810.1. NC_011726.1.

3D structure databases

ProteinModelPortaliB7JVW5.
SMRiB7JVW5. Positions 9-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi41431.PCC8801_1602.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACK65654; ACK65654; PCC8801_1602.
KEGGicyp:PCC8801_1602.
PATRICi21575449. VBICyaSp125535_1757.

Phylogenomic databases

eggNOGiCOG1850.
HOGENOMiHOG000230831.
KOiK01601.
OMAiHAAFTRN.
OrthoDBiEOG6ZKXMS.

Enzyme and pathway databases

BioCyciCSP41431:GHLK-1613-MONOMER.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1.
InterProiIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Novel metabolic attributes of the genus Cyanothece, comprising a group of unicellular nitrogen-fixing Cyanobacteria."
    Bandyopadhyay A., Elvitigala T., Welsh E., Stockel J., Liberton M., Min H., Sherman L.A., Pakrasi H.B.
    MBio 2:E214-E214(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: PCC 8801Imported.

Entry informationi

Entry nameiB7JVW5_CYAP8
AccessioniPrimary (citable) accession number: B7JVW5
Entry historyi
Integrated into UniProtKB/TrEMBL: February 10, 2009
Last sequence update: February 10, 2009
Last modified: May 27, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.