B7JN71 (THIM_BACC0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyethylthiazole kinase
EC=2.7.1.50

Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name=TH kinase
Short name=Thz kinase

Gene names

Name:	thiM
Ordered Locus Names:	BCAH820_0419

Organism

Bacillus cereus (strain AH820) [Complete proteome] [HAMAP]

Taxonomic identifier

405535 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cereus

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole. HAMAP-Rule MF_00228
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00228
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole: step 1/1. HAMAP-Rule MF_00228
Sequence similarities	Belongs to the Thz kinase family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW thiamine diphosphate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP hydroxyethylthiazole kinase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Hydroxyethylthiazole kinase HAMAP-Rule MF_00228		PRO_1000198106
Sites
	Binding site	45	1	Substrate; via amide nitrogen By similarity
	Binding site	121	1	ATP By similarity
	Binding site	167	1	ATP By similarity
	Binding site	194	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7JN71 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: B53502FADB94651B	FASTA	268	28,059
	10 20 30 40 50 60 MNTKEISKVV DLVRESNPLV HNITNVVVTN FTANGLLALG ASPVMAYAKE EVAEMASIAG 70 80 90 100 110 120 ALVLNMGTLR PDEVEAMLLA GKSANRNDVP VLFDPVGAGA TSYRTEVARH IPAEIELAII 130 140 150 160 170 180 RGNAAEIANV INEKWEIKGV DAGAGNGNVV SIAKQAADEL NTVAVITGKE DVVTDGERTI 190 200 210 220 230 240 VIRNGHSILT KITGTGCLLT SVIGAFVAVE KDYVKAAVAA LTFYGVAAEL AAAKTVEKGP 250 260 GSFQIEFLNQ LANTTSGDIE KYGKIEVI

References

[1]	"Genome sequence of Bacillus cereus AH820." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AH820.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001283 Genomic DNA. Translation: ACK92259.1.
RefSeq	YP_002449411.1. NC_011773.1.
3D structure databases
ProteinModelPortal	B7JN71.
SMR	B7JN71. Positions 1-268.
ModBase	Search...
Protein-protein interaction databases
STRING	405535.BCAH820_0419.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACK92259; ACK92259; BCAH820_0419.
GeneID	7188325.
KEGG	bcu:BCAH820_0419.
PATRIC	18837668. VBIBacCer122868_0391.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2145.
HOGENOM	HOG000114352.
KO	K00878.
OMA	SPVMAHA.
ProtClustDB	PRK09355.
Enzyme and pathway databases
BioCyc	BCER405535:GHSL-429-MONOMER.
UniPathway	UPA00060; UER00139.
Family and domain databases
HAMAP	MF_00228. Thz_kinase.
InterPro	IPR000417. Hyethyz_kinase. [Graphical view]
Pfam	PF02110. HK. 1 hit. [Graphical view]
PIRSF	PIRSF000513. Thz_kinase. 1 hit.
PRINTS	PR01099. HYETHTZKNASE.
TIGRFAMs	TIGR00694. thiM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THIM_BACC0

Accession

Primary (citable) accession number: B7JN71

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 29, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program