Reviewed, UniProtKB/Swiss-Prot B7JM28 (DXS_BACC0)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	BCAH820_4197

Organism

Bacillus cereus (strain AH820) [Complete proteome] [HAMAP]

Taxonomic identifier

405535 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	630 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 630	630	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000119537

Sequences

	Sequence		Length	Mass (Da)
	B7JM28-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 29320ADE58BB6B1D	FASTA	630	69,427
	10 20 30 40 50 60 MDLTQIQNPS FLKDMSISEL EGLSEDIRKF LIEELSQTGG HIAPNLGVVE LTIALHKLFD 70 80 90 100 110 120 SPKDKFLWDV GHQSYVHKIL TGRAKEFGTL RQYQGLCGFP KRCESEHDVW ETGHSSTSLS 130 140 150 160 170 180 AAMGMALARD LKKTKEYVIP IIGDGALTGG MALEALNHIG HEKTDMIVIL NDNEMSIAPN 190 200 210 220 230 240 VGALHNVLGR LRTAGKYHWV KDELEYILKK IPAVGGKVAA TAEKIKDSLK YLLVSGVFFE 250 260 270 280 290 300 ELGFTYLGPV DGHDYEKLFE TLQYAKKTKG PVLVHVITKK GKGYKPAESD VIGTWHGTGP 310 320 330 340 350 360 YKIESGDFVK PKEVAPAWSA VVSETVLKLA RTDERIVAIT PAMPVGSKLE KFQKEFPDRM 370 380 390 400 410 420 IDVGIAEQHA TTMAAGMATQ GMKPFLAIYS TFLQRAYDQV VHDICRQNLN VFIGIDRSGL 430 440 450 460 470 480 VGADGETHQG VFDISFLRHL PNMVIMMPKD ENEGQHLVYT AMQYEDGPIA LRYARGNGLG 490 500 510 520 530 540 VHMDEELKAI PIGSWETLKE GTQAAILTFG TTIPMAMEAA ERLEKAGVSV KVVNARFIKP 550 560 570 580 590 600 MDEAYLHDLL GKNIPILTIE EACLIGGFGT GVVEFASENG YHSALVERMG IPDRFIEHGS 610 620 630 VTKLLEEIGL TTDAVVDRIH TMIPSKQKRA

References

[1]	"Genome sequence of Bacillus cereus AH820." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001283 Genomic DNA. Translation: ACK88884.1.
RefSeq	YP_002453147.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7190911.
GenomeReviews	Gene locus BCAH820_4197 in contig CP001283_GR.
KEGG	bcu:BCAH820_4197.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BACC0

Accession

Primary (citable) accession number: B7JM28

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	September 22, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)