B7JFQ8 (PHNX_BACC0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphonoacetaldehyde hydrolase
Short name=Phosphonatase
EC=3.11.1.1

Alternative name(s):
Phosphonoacetaldehyde phosphonohydrolase

Gene names

Name:	phnX
Ordered Locus Names:	BCAH820_1414

Organism

Bacillus cereus (strain AH820) [Complete proteome] [HAMAP]

Taxonomic identifier

405535 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cereus

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in phosphonate degradation By similarity.
Catalytic activity	Phosphonoacetaldehyde + H2O = acetaldehyde + phosphate. HAMAP-Rule MF_01375
Cofactor	Binds 1 Mg2+ ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. PhnX family.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Schiff base
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	organic phosphonate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: InterPro phosphonoacetaldehyde hydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Phosphonoacetaldehyde hydrolase HAMAP-Rule MF_01375		PRO_1000144826
Sites
	Active site	9	1	Nucleophile By similarity
	Active site	50	1	Schiff-base intermediate with substrate By similarity
	Metal binding	9	1	Magnesium By similarity
	Metal binding	11	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	183	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7JFQ8 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: DE04AD84BE0E9033	FASTA	264	30,032
	10 20 30 40 50 60 MKIEAVIFDW AGTTVDYGCF APLEVFMEIF HKRGVGITAE EARKPMGLLK IDHVRALTEM 70 80 90 100 110 120 PRIANEWNRI FGQLPTETDI QEMYEEFEEI LFAILPRYAS PIHGVKEVIA SLRERGIKIG 130 140 150 160 170 180 STTGYTREMM DIVAKEAALQ GYKPDFLVTP DDVPAGRPYP WMCYKNAMEL GVYPMNHMIK 190 200 210 220 230 240 IGDTVSDMKE GRNAGMWTVG VILGSSELGL SEEEVENMDS AELREKIEVV RNRFVENGAH 250 260 FTIETMQELE SVMERIEKQE LIIS

References

[1]	"Genome sequence of Bacillus cereus AH820." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AH820.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001283 Genomic DNA. Translation: ACK88943.1.
RefSeq	YP_002450365.1. NC_011773.1.
3D structure databases
ProteinModelPortal	B7JFQ8.
ModBase	Search...
Protein-protein interaction databases
STRING	405535.BCAH820_1414.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACK88943; ACK88943; BCAH820_1414.
GeneID	7189447.
KEGG	bcu:BCAH820_1414.
PATRIC	18839684. VBIBacCer122868_1355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0637.
HOGENOM	HOG000217971.
KO	K05306.
OMA	EFGATWE.
ProtClustDB	PRK13478.
Enzyme and pathway databases
BioCyc	BCER405535:GHSL-2116-MONOMER.
Family and domain databases
Gene3D	1.10.150.240. 1 hit. 3.40.50.1000. 2 hits.
HAMAP	MF_01375. PhnX.
InterPro	IPR023214. HAD-like_dom. IPR006439. HAD-SF_hydro_IA_v1. IPR006402. HAD-SF_hydro_IA_v3. IPR023198. PGP_dom2. IPR006323. Phosphonoacetald_hydro. [Graphical view]
Pfam	PF13419. HAD_2. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01549. HAD-SF-IA-v1. 1 hit. TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01422. phosphonatase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHNX_BACC0

Accession

Primary (citable) accession number: B7JFQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program