Reviewed, UniProtKB/Swiss-Prot B7JDF1 (GLGB_BACC0)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	BCAH820_4980

Organism

Bacillus cereus (strain AH820) [Complete proteome] [HAMAP]

Taxonomic identifier

405535 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_1000131807
Sites
	Active site	204	1	By similarity
	Active site	239	1	By similarity
	Active site	244	1	By similarity
	Active site	307	1	By similarity
	Active site	309	1	By similarity
	Active site	352	1	By similarity
	Active site	419	1	By similarity
	Active site	420	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7JDF1-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 3437A2EFCB8320C0	FASTA	645	76,105
	10 20 30 40 50 60 MSVINCEEVK RDEFHTEKYY ESYNIFGAHI VTEDEMRGVR FTVWAPHAKA MSVVGDFNEW 70 80 90 100 110 120 DYEQHKMLQV TEEGIWSLFI PHIEEREIYK YAIETMAGDV IFKADPYAVY AEVRPNTASV 130 140 150 160 170 180 VFDIKGYEWN DKNWSRKKKK KSVYKEAMTV YELHFGSWKK KEDGTLYSYR EMAEELIPYV 190 200 210 220 230 240 VEHQFTHIEI MPLVEHPYDR SWGYQGTGYY AATSRFGTPY DLMHFVDECH KYGIGVILDW 250 260 270 280 290 300 VPGHFCKDAH GLYLFDGTPT YEYKDKDVQE NPVWGTVNFD LGKREVRNFL ISNALFWMRY 310 320 330 340 350 360 FHIDGFRVDA VANMLYWNKE GQEQSNEHAV SFLRELNEAV FAEDEDFLMT AEDSTAWPLV 370 380 390 400 410 420 TAPTYEGGLG FNYKWNMGWM NDVLKYMECA PEYRKYIHDK MTFSLLYAYS ENFILPLSHD 430 440 450 460 470 480 EVVHGKKSLL NKMPGDYWDK FAQLRLLYGY FFTHPGKKLL FMGGEFGQFD EWKDLEDLDW 490 500 510 520 530 540 NLHDFEMHRY MHDYFKELIA LYKRSKPLWQ LDHSREGFQW IDANNNEQSI FSFIRQGDKQ 550 560 570 580 590 600 EDALVVVCNF TKATYENYKV GVPDFEYYNE ILNSDAEQYG GSGQVNKKRL KTIQEPYHNQ 610 620 630 640 TAHVEITIPP FGVSILRPVK TRKGSKKQDG SKTKVRSNVT SRGKR

References

[1]	"Genome sequence of Bacillus cereus AH820." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001283 Genomic DNA. Translation: ACK88551.1.
RefSeq	YP_002453926.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7191798.
GenomeReviews	Gene locus BCAH820_4980 in contig CP001283_GR.
KEGG	bcu:BCAH820_4980.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BACC0

Accession

Primary (citable) accession number: B7JDF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 6 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)