B7JDF1 (GLGB_BACC0) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	BCAH820_4980

Organism

Bacillus cereus (strain AH820) [Complete proteome] [HAMAP]

Taxonomic identifier

405535 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group › Bacillus cereus

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: UniProtKB-HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685		PRO_1000131807
Sites
	Active site	309	1	Nucleophile By similarity
	Active site	352	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7JDF1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 3437A2EFCB8320C0	FASTA	645	76,105
	10 20 30 40 50 60 MSVINCEEVK RDEFHTEKYY ESYNIFGAHI VTEDEMRGVR FTVWAPHAKA MSVVGDFNEW 70 80 90 100 110 120 DYEQHKMLQV TEEGIWSLFI PHIEEREIYK YAIETMAGDV IFKADPYAVY AEVRPNTASV 130 140 150 160 170 180 VFDIKGYEWN DKNWSRKKKK KSVYKEAMTV YELHFGSWKK KEDGTLYSYR EMAEELIPYV 190 200 210 220 230 240 VEHQFTHIEI MPLVEHPYDR SWGYQGTGYY AATSRFGTPY DLMHFVDECH KYGIGVILDW 250 260 270 280 290 300 VPGHFCKDAH GLYLFDGTPT YEYKDKDVQE NPVWGTVNFD LGKREVRNFL ISNALFWMRY 310 320 330 340 350 360 FHIDGFRVDA VANMLYWNKE GQEQSNEHAV SFLRELNEAV FAEDEDFLMT AEDSTAWPLV 370 380 390 400 410 420 TAPTYEGGLG FNYKWNMGWM NDVLKYMECA PEYRKYIHDK MTFSLLYAYS ENFILPLSHD 430 440 450 460 470 480 EVVHGKKSLL NKMPGDYWDK FAQLRLLYGY FFTHPGKKLL FMGGEFGQFD EWKDLEDLDW 490 500 510 520 530 540 NLHDFEMHRY MHDYFKELIA LYKRSKPLWQ LDHSREGFQW IDANNNEQSI FSFIRQGDKQ 550 560 570 580 590 600 EDALVVVCNF TKATYENYKV GVPDFEYYNE ILNSDAEQYG GSGQVNKKRL KTIQEPYHNQ 610 620 630 640 TAHVEITIPP FGVSILRPVK TRKGSKKQDG SKTKVRSNVT SRGKR

References

[1]	"Genome sequence of Bacillus cereus AH820." Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AH820.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001283 Genomic DNA. Translation: ACK88551.1.
RefSeq	YP_002453926.1. NC_011773.1.
3D structure databases
ProteinModelPortal	B7JDF1.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	405535.BCAH820_4980.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACK88551; ACK88551; BCAH820_4980.
GeneID	7191798.
KEGG	bcu:BCAH820_4980.
PATRIC	18846943. VBIBacCer122868_4976.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HOG000283037.
KO	K00700.
OMA	AKLLFMG.
OrthoDB	EOG6JX7GT.
Enzyme and pathway databases
BioCyc	BCER405535:GHSL-4993-MONOMER.
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006048. A-amylase_b_C. IPR006407. GlgB. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. SSF51445. 1 hit.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BACC0

Accession

Primary (citable) accession number: B7JDF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program