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B7JDF1 (GLGB_BACC0) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:BCAH820_4980
OrganismBacillus cereus (strain AH820) [Complete proteome] [HAMAP]
Taxonomic identifier405535 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Protein attributes

Sequence length645 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6456451,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000131807

Sites

Active site3091Nucleophile By similarity
Active site3521Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
B7JDF1 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 3437A2EFCB8320C0

FASTA64576,105
        10         20         30         40         50         60 
MSVINCEEVK RDEFHTEKYY ESYNIFGAHI VTEDEMRGVR FTVWAPHAKA MSVVGDFNEW 

        70         80         90        100        110        120 
DYEQHKMLQV TEEGIWSLFI PHIEEREIYK YAIETMAGDV IFKADPYAVY AEVRPNTASV 

       130        140        150        160        170        180 
VFDIKGYEWN DKNWSRKKKK KSVYKEAMTV YELHFGSWKK KEDGTLYSYR EMAEELIPYV 

       190        200        210        220        230        240 
VEHQFTHIEI MPLVEHPYDR SWGYQGTGYY AATSRFGTPY DLMHFVDECH KYGIGVILDW 

       250        260        270        280        290        300 
VPGHFCKDAH GLYLFDGTPT YEYKDKDVQE NPVWGTVNFD LGKREVRNFL ISNALFWMRY 

       310        320        330        340        350        360 
FHIDGFRVDA VANMLYWNKE GQEQSNEHAV SFLRELNEAV FAEDEDFLMT AEDSTAWPLV 

       370        380        390        400        410        420 
TAPTYEGGLG FNYKWNMGWM NDVLKYMECA PEYRKYIHDK MTFSLLYAYS ENFILPLSHD 

       430        440        450        460        470        480 
EVVHGKKSLL NKMPGDYWDK FAQLRLLYGY FFTHPGKKLL FMGGEFGQFD EWKDLEDLDW 

       490        500        510        520        530        540 
NLHDFEMHRY MHDYFKELIA LYKRSKPLWQ LDHSREGFQW IDANNNEQSI FSFIRQGDKQ 

       550        560        570        580        590        600 
EDALVVVCNF TKATYENYKV GVPDFEYYNE ILNSDAEQYG GSGQVNKKRL KTIQEPYHNQ 

       610        620        630        640 
TAHVEITIPP FGVSILRPVK TRKGSKKQDG SKTKVRSNVT SRGKR 

« Hide

References

[1]"Genome sequence of Bacillus cereus AH820."
Dodson R.J., Durkin A.S., Rosovitz M.J., Rasko D.A., Hoffmaster A., Ravel J., Sutton G.
Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AH820.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001283 Genomic DNA. Translation: ACK88551.1.
RefSeqYP_002453926.1. NC_011773.1.

3D structure databases

ProteinModelPortalB7JDF1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405535.BCAH820_4980.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK88551; ACK88551; BCAH820_4980.
GeneID7191798.
KEGGbcu:BCAH820_4980.
PATRIC18846943. VBIBacCer122868_4976.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycBCER405535:GHSL-4993-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_BACC0
AccessionPrimary (citable) accession number: B7JDF1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: June 11, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries