B7JBI1 (GSA_ACIF2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate-1-semialdehyde 2,1-aminomutase
Short name=GSA
EC=5.4.3.8

Alternative name(s):
Glutamate-1-semialdehyde aminotransferase
Short name=GSA-AT

Gene names

Name:	hemL
Ordered Locus Names:	AFE_3303

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus › Thiobacillus ferrooxidans

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate. HAMAP-Rule MF_00375
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. HAMAP-Rule MF_00375
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00375.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: HAMAP protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Glutamate-1-semialdehyde 2,1-aminomutase HAMAP-Rule MF_00375		PRO_1000121851
Amino acid modifications
	Modified residue	266	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7JBI1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 5D79E349A3FECCB9	FASTA	430	45,193
	10 20 30 40 50 60 MTQTSHALFH MAQQCIPGGV NSPVRAFRGV GGDPIFIDHA EGPFFWDVEG KRYLDYVGSW 70 80 90 100 110 120 GPMIHGHGHP EVLAAVHAQV NKGLGFGAPT AIEVEMAELV CALVPGIESV RMTSSGTEAV 130 140 150 160 170 180 MTAIRLARGY TGRDRIIKFE GNYHGHSDSL LVKAGSGALT LGQPSSAGVP REVSQNTLVL 190 200 210 220 230 240 PYNDLPAVVE MMAQFGFDVA TIIVEPVAGN MGCVPPEPGF LEGLRAVCDQ YGCVLIFDEV 250 260 270 280 290 300 MTGFRVALGG AQALYGVRPD LTTLGKIIGG GLPVGAVGGP REIMEYLAPT GPVYQAGTLS 310 320 330 340 350 360 GNPVAMAAGL ATLRLLTVPG FHERLAAQTA VLCEGLAERA EAAGVPMQIN HVPGMFGWFF 370 380 390 400 410 420 AEQPVRGFDT VMAADSKRYA RFFHGLLARG VYLAPSAYEA GFLSAAHGDT EIAATLDAAE 430 AVLATLKEDA

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001219 Genomic DNA. Translation: ACK79485.1.
RefSeq	YP_002427656.1. NC_011761.1.
3D structure databases
ProteinModelPortal	B7JBI1.
ModBase	Search...
Protein-protein interaction databases
STRING	243159.AFE_3303.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACK79485; ACK79485; AFE_3303.
GeneID	7136891.
KEGG	afr:AFE_3303.
PATRIC	20657535. VBIAciFer29821_3010.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0001.
HOGENOM	HOG000020210.
KO	K01845.
OMA	FNGNPIS.
ProtClustDB	PRK00062.
Enzyme and pathway databases
BioCyc	AFER243159:GH3S-1703-MONOMER.
UniPathway	UPA00251; UER00317.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
HAMAP	MF_00375. HemL_aminotrans_3.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_ACIF2

Accession

Primary (citable) accession number: B7JBI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program