Reviewed, UniProtKB/Swiss-Prot B7J9P7 (DXR_ACIF2)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose 5-phosphate reductoisomerase
      Short name=DXP reductoisomerase
    EC=1.1.1.267
Alternative name(s):
    1-deoxyxylulose-5-phosphate reductoisomerase
    2-C-methyl-D-erythritol 4-phosphate synthase

Gene names

Name:	dxr
Ordered Locus Names:	AFE_1450

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADP-dependent rearrangement and reduction of 1-deoxy-D-xylulose-5-phosphate (DXP) to 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity.
Catalytic activity	2-C-methyl-D-erythritol 4-phosphate + NADP+ = 1-deoxy-D-xylulose 5-phosphate + NADPH. HAMAP MF_00183
Cofactor	Divalent cation By similarity.
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 1/6. HAMAP MF_00183
Sequence similarities	Belongs to the DXR family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate reductoisomerase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	1-deoxy-D-xylulose 5-phosphate reductoisomerase HAMAP MF_00183		PRO_1000118490
Regions
	Nucleotide binding	10 – 39	30	NADP By similarity
Sites
	Metal binding	154	1	Divalent metal cation By similarity
	Metal binding	156	1	Divalent metal cation By similarity
	Metal binding	223	1	Divalent metal cation By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	201	1	Substrate By similarity
	Binding site	223	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7J9P7-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: A4459AA052635932	FASTA	393	42,127
	10 20 30 40 50 60 MEMTRGICIL GATGSIGKST LDVVSRHPDQ FRIVALTGNH RVAEMQLLCQ QHHPELVVMA 70 80 90 100 110 120 APEAAQQLRV GLGDAGLKKI QVESGPEALA EAARMSGVDE VMAAIVGAAG LLPTLAAVEA 130 140 150 160 170 180 GKKVYLANKE CLVMAGNLFM ERVRQHQVTL LPIDSEHNAV FQCFADGKGV RRILLTASGG 190 200 210 220 230 240 PFRTWPAEHL AVVTPDQACA HPNWVMGRKI SVDSATMMNK GLEVIEAHWL FDLPASRIDV 250 260 270 280 290 300 MIHPQSIIHS MVEYVDGSVL AQLGNPDMRT PIAHALAFPE RMESGVSSLD LAHGPDLQFE 310 320 330 340 350 360 APDLQRFPCL ALAFDALQAG GAAATVLNAA NEIAVQAFLE GHLPFLRIAA VVEDTLGELQ 370 380 390 PAAPDHLDDV LAIDQLAREV ALRHLARHGS GMQ

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001219 Genomic DNA. Translation: ACK78432.1.
RefSeq	YP_002425878.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7135018.
GenomeReviews	Gene locus AFE_1450 in contig CP001219_GR.
KEGG	afr:AFE_1450.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00183. [Tree]
InterPro	IPR003821. DXP_reductoisomerase. IPR013644. DXP_reductoisomerase_C. IPR013512. DXP_reductoisomerase_N. [Graphical view]
Pfam	PF08436. DXP_redisom_C. 1 hit. PF02670. DXP_reductoisom. 1 hit. [Graphical view]
PIRSF	PIRSF006205. Dxp_reductismrs. 1 hit.
TIGRFAMs	TIGR00243. Dxr. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DXR_ACIF2

Accession

Primary (citable) accession number: B7J9P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	September 22, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)