Reviewed, UniProtKB/Swiss-Prot B7J7U7 (TPIS_ACIF2)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	AFE_2633

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	257 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 257	257	Triosephosphate isomerase HAMAP MF_00147		PRO_1000117993
Sites
	Active site	95	1	Electrophile By similarity
	Active site	168	1	Proton acceptor By similarity
	Binding site	9	1	Substrate By similarity
	Binding site	11	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7J7U7-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 4EBFF9C0F036D50D	FASTA	257	27,314
	10 20 30 40 50 60 MRPTMVAGNW KMNGLSGDAV HLTQAILHAG LEPMRPEVVI FPPFTLLHAV SQEAKSSALR 70 80 90 100 110 120 WGGQNLFWEA SGAYTGEISG AMLRDMGCRY VLIGHSERRQ IFAESDAQIV QKIKAALLSG 130 140 150 160 170 180 LIPVVCVGET EAERAQGLTD AVLRRQLEAV LPLLNLEASQ PNLIIAYEPV WAIGTGLSAS 190 200 210 220 230 240 PEQAQAVHVF IRELAAAYSA QLARRLLLLY GGSVKGNNAA ALFDQADIDG ALVGGASLQA 250 GEFIQICRAA ESAGRGG

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001219 Genomic DNA. Translation: ACK80228.1.
RefSeq	YP_002427010.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7136961.
GenomeReviews	Gene locus AFE_2633 in contig CP001219_GR.
KEGG	afr:AFE_2633.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_ACIF2

Accession

Primary (citable) accession number: B7J7U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)