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B7J7N7

- LIPA_ACIF2

UniProt

B7J7N7 - LIPA_ACIF2

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Protein
Lipoyl synthase
Gene
lipA, AFE_2570
Organism
Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi97 – 971Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAFER243159:GH3S-2565-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:AFE_2570
OrganismiAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
Taxonomic identifieri243159 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus
ProteomesiUP000001362: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Lipoyl synthaseUniRule annotation
PRO_1000191443Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243159.AFE_2570.

Structurei

3D structure databases

ProteinModelPortaliB7J7N7.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B7J7N7-1 [UniParc]FASTAAdd to Basket

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MDSQPQSKKA ARGADKTARN PIPIIPAPTE RLPKPQWLRV RSPLSPEVDQ    50
LKKILRDAAL HTVCEEASCP NLGECFGGGT ATFMILGDIC TRRCPFCDVA 100
HGRPEAPDPL ESVHLARTVA SMKLRFVVIT SVDRDDLRDG GARHFAEVIS 150
ALREHCPELH VEILVPDFRG RVANALAAFR ETPPDVFNHN LETVPRLYLQ 200
ARPGADYHHS LQLLAAFKAQ HPQIPTKSGL MLGLGEEIDE IRGVMRDLRQ 250
AGCELLTIGQ YLAPSRHHLP VARFVPPEEF QQLQRDGMAM GFRHVASGPL 300
VRSSYHAERS FHEIGGD 317
Length:317
Mass (Da):35,148
Last modified:February 10, 2009 - v1
Checksum:iFCBF3A7FB750C857
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001219 Genomic DNA. Translation: ACK80883.1.
RefSeqiYP_002426950.1. NC_011761.1.

Genome annotation databases

EnsemblBacteriaiACK80883; ACK80883; AFE_2570.
GeneIDi7136876.
KEGGiafr:AFE_2570.
PATRICi20656155. VBIAciFer29821_2334.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001219 Genomic DNA. Translation: ACK80883.1 .
RefSeqi YP_002426950.1. NC_011761.1.

3D structure databases

ProteinModelPortali B7J7N7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243159.AFE_2570.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK80883 ; ACK80883 ; AFE_2570 .
GeneIDi 7136876.
KEGGi afr:AFE_2570.
PATRICi 20656155. VBIAciFer29821_2334.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci AFER243159:GH3S-2565-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications."
    Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S.
    BMC Genomics 9:597-597(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

Entry informationi

Entry nameiLIPA_ACIF2
AccessioniPrimary (citable) accession number: B7J7N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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