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B7J7N7

- LIPA_ACIF2

UniProt

B7J7N7 - LIPA_ACIF2

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Protein

Lipoyl synthase

Gene

lipA

Organism
Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi90 – 901Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi97 – 971Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAFER243159:GH3S-2565-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-synUniRule annotation
Short name:
LSUniRule annotation
Lipoate synthaseUniRule annotation
Lipoic acid synthaseUniRule annotation
Sulfur insertion protein LipAUniRule annotation
Gene namesi
Name:lipAUniRule annotation
Ordered Locus Names:AFE_2570
OrganismiAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
Taxonomic identifieri243159 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus
ProteomesiUP000001362: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Lipoyl synthasePRO_1000191443Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243159.AFE_2570.

Structurei

3D structure databases

ProteinModelPortaliB7J7N7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

B7J7N7 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSQPQSKKA ARGADKTARN PIPIIPAPTE RLPKPQWLRV RSPLSPEVDQ
60 70 80 90 100
LKKILRDAAL HTVCEEASCP NLGECFGGGT ATFMILGDIC TRRCPFCDVA
110 120 130 140 150
HGRPEAPDPL ESVHLARTVA SMKLRFVVIT SVDRDDLRDG GARHFAEVIS
160 170 180 190 200
ALREHCPELH VEILVPDFRG RVANALAAFR ETPPDVFNHN LETVPRLYLQ
210 220 230 240 250
ARPGADYHHS LQLLAAFKAQ HPQIPTKSGL MLGLGEEIDE IRGVMRDLRQ
260 270 280 290 300
AGCELLTIGQ YLAPSRHHLP VARFVPPEEF QQLQRDGMAM GFRHVASGPL
310
VRSSYHAERS FHEIGGD
Length:317
Mass (Da):35,148
Last modified:February 10, 2009 - v1
Checksum:iFCBF3A7FB750C857
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001219 Genomic DNA. Translation: ACK80883.1.
RefSeqiWP_012537264.1. NC_011761.1.
YP_002426950.1. NC_011761.1.

Genome annotation databases

EnsemblBacteriaiACK80883; ACK80883; AFE_2570.
GeneIDi7136876.
KEGGiafr:AFE_2570.
PATRICi20656155. VBIAciFer29821_2334.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001219 Genomic DNA. Translation: ACK80883.1 .
RefSeqi WP_012537264.1. NC_011761.1.
YP_002426950.1. NC_011761.1.

3D structure databases

ProteinModelPortali B7J7N7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243159.AFE_2570.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK80883 ; ACK80883 ; AFE_2570 .
GeneIDi 7136876.
KEGGi afr:AFE_2570.
PATRICi 20656155. VBIAciFer29821_2334.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci AFER243159:GH3S-2565-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications."
    Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S.
    BMC Genomics 9:597-597(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

Entry informationi

Entry nameiLIPA_ACIF2
AccessioniPrimary (citable) accession number: B7J7N7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: October 29, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3