B7J781 (AROA_ACIF2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	AFE_0901

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000118773

Sequences

	Sequence		Length	Mass (Da)
	B7J781 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: C2C6F1F7220BFEC8	FASTA	433	44,810
	10 20 30 40 50 60 MTRYLVRPGS RLAGRFPVPG DKSISHRAVI LGALAEGVTE VEGLLEGADV LATIAAFRSM 70 80 90 100 110 120 GVQMEGPDKG HLRIHGAGLQ GLRAPVVPLD CGNSGTAMRL LAGVLAGQPF PSTLVGDASL 130 140 150 160 170 180 QKRPMGRILN PLRAMGAEIA AQDGRAPLHI HGRPLHGIDY ALPVASAQVK SAVLLAGLYA 190 200 210 220 230 240 DGQTCVTEPA PTRDHSERML QGFGQPVERH GPRACLRGGG RLCGQALQVP GDISSAAFFL 250 260 270 280 290 300 LGATIAPGSD LTLEGVGINP TRTGIIEILT RMGARIDLTA LREVGGEPVA DIRVRYAPLQ 310 320 330 340 350 360 GIAIPPRLVP LAIDEFPALF IAAACAKGQT VITGAEELRV KESDRIAVMA GGLRALGATV 370 380 390 400 410 420 EERVDGAIIS GSALLGGRVD SHGDHRIAMA FAMAALVAQG DMEILDCANV ATSFPSFPAL 430 AQQAGLLLEV ASA

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001219 Genomic DNA. Translation: ACK78170.1.
RefSeq	YP_002425379.1. NC_011761.1.
3D structure databases
ProteinModelPortal	B7J781.
ModBase	Search...
Protein-protein interaction databases
STRING	B7J781.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7135906.
GenomeReviews	Gene locus AFE_0901 in contig CP001219_GR.
KEGG	afr:AFE_0901.
PATRIC	20653113. VBIAciFer29821_0864.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	LECANIS.
ProtClustDB	CLSK2407745.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_ACIF2

Accession

Primary (citable) accession number: B7J781

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program