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B7J6C4 (DAPA_ACIF2) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 21. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:AFE_2358
OrganismAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]
Taxonomic identifier243159 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291Dihydrodipicolinate synthase HAMAP MF_00418
PRO_1000124012

Regions

Region48 – 492Pyruvate binding By similarity

Sites

Active site1611Schiff-base intermediate with substrate By similarity
Binding site1061Pyruvate By similarity
Site1331Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
B7J6C4 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: BC3817F5E90F8D3F

FASTA29131,199
        10         20         30         40         50         60 
MFHGSMVALV TPMQVDGAID DVALRELVEW HIAEGTHALV AVGTTGESAT LEMREHVAVI 

        70         80         90        100        110        120 
QTVVEQARGR VPVIAGTGAN ATHEAIELTR AAMEVKADAA LLVSPYYNKP TQEGLFQHYS 

       130        140        150        160        170        180 
AIAEHCHFPI ILYNVPGRTA GDILPETVAR LAPRADIIGI KEASGKVERV AEILALCGDQ 

       190        200        210        220        230        240 
VQVYSGDDGA ALAAMALGAR GVISVTANAA PRLMARMCDL ALAGDFVGAR AVNAQLTGLH 

       250        260        270        280        290 
RDLFLESNPI PVKWALHEMG RMESVLRLPL TTLSSVHHER LRESLRRAQC I

« Hide

References

[1]"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications."
Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S.
BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001219 Genomic DNA. Translation: ACK78144.1.
RefSeqYP_002426749.1. NC_011761.1.

3D structure databases

ProteinModelPortalB7J6C4.
ModBaseSearch...

Protein-protein interaction databases

STRINGB7J6C4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7135524.
GenomeReviewsGene locus AFE_2358 in contig CP001219_GR.
KEGGafr:AFE_2358.
PATRIC20655779. VBIAciFer29821_2148.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG358848.
OMAFMLCGGH.
ProtClustDBCLSK2408286.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_ACIF2
AccessionPrimary (citable) accession number: B7J6C4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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