Reviewed, UniProtKB/Swiss-Prot B7J6C1 (PUR7_ACIF2)

Names and origin

Protein names

Recommended name:
    Phosphoribosylaminoimidazole-succinocarboxamide synthase
    EC=6.3.2.6
Alternative name(s):
    SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	AFE_2355

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	240 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 240	240	Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137		PRO_1000117820

Sequences

	Sequence		Length	Mass (Da)
	B7J6C1-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: F80951EA302BE3CE	FASTA	240	27,236
	10 20 30 40 50 60 MNERIALYEG KAKIVYASES PDELVLHFKD DTSAFDGEKV EQLAHKGEIN NAFNAFIMEY 70 80 90 100 110 120 LQGEGVPTHF IRRLNARESL VRRLEMIPVE CVVRNRAAGS LSKRLGIEEG RVLEPPTLEF 130 140 150 160 170 180 FLKNDALHDP MINTSHIRTF GWATAEEVEA MRRWTMRVNM LLSALFAKAN LILVDFKLEF 190 200 210 220 230 240 GRFDGELYLG DEFSPDGCRL WDALSLEKMD KDRFRRGLGG VVEAYAEVAR RLGVPLPAAS

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001219 Genomic DNA. Translation: ACK78311.1.
RefSeq	YP_002426746.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7137078.
GenomeReviews	Gene locus AFE_2355 in contig CP001219_GR.
KEGG	afr:AFE_2355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG306070.
OMA	TAFNAQK.
Family and domain databases
HAMAP	MF_00137. SAICAR_synth. [Tree]
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synt. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00081. purC. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR7_ACIF2

Accession

Primary (citable) accession number: B7J6C1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	February 9, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)