Reviewed, UniProtKB/Swiss-Prot B7J5I9 (AMPA_ACIF2)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	AFE_2201

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000118444
Sites
	Active site	281	1	Potential
	Active site	355	1	Potential
	Metal binding	269	1	Manganese 2 By similarity
	Metal binding	274	1	Manganese 1 By similarity
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	292	1	Manganese 2 By similarity
	Metal binding	351	1	Manganese 1 By similarity
	Metal binding	353	1	Manganese 1 By similarity
	Metal binding	353	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7J5I9-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 7E950A380AC66BF1	FASTA	500	52,844
	10 20 30 40 50 60 MEIAVQCQNP TTDNSDCVVV GIYEGGILSP AATLVDLASG GALRALLDTG DFTGDCGDTQ 70 80 90 100 110 120 LLYQVPGMAA ARVLVLGLGS HGKVKDSQFR KAALAAARAL QGARVGRASL HLLDTPVIRR 130 140 150 160 170 180 SAPACAKILV QAVADAEYHF DRHKKPADAP QRPISELQLS ISENDTAALA ELQGAVAEAQ 190 200 210 220 230 240 ATARAVAWTR DMANEPGNIC TPTWLAEQAE AMAGRLGIKS TILGPDAMEA LGMHLLLGVA 250 260 270 280 290 300 HGSRQPPRLI ILEYRGGAEN QAPIVLVGKG ITFDAGGISL KPADKMDEMK YDMCGGASAL 310 320 330 340 350 360 AAIQAAAELQ LPLNIVTVVP ASENLPDGQA TKPGDIHRSM NGLSVEVVNT DAEGRLILAD 370 380 390 400 410 420 TLTYVERFEP DVVIDMATLT GACIIALGHQ TAAVMGNHEG LVHDLIAAGK ESMDRVWELP 430 440 450 460 470 480 LFEEYQEQLK SPVADLSNVG GRPAGTITAA CFLSRFTENY RWAHLDIAGV AWKSGEHKGA 490 500 TGRPVPLLVE YLLRRARQVA

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001219 Genomic DNA. Translation: ACK79866.1.
RefSeq	YP_002426600.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7135385.
GenomeReviews	Gene locus AFE_2201 in contig CP001219_GR.
KEGG	afr:AFE_2201.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00181. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_ACIF2

Accession

Primary (citable) accession number: B7J5I9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)