Reviewed, UniProtKB/Swiss-Prot B7J4J4 (PPNK_ACIF2)

Names and origin

Protein names

Recommended name:
    Probable inorganic polyphosphate/ATP-NAD kinase
      Short name=Poly(P)/ATP NAD kinase
    EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	AFE_0451

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity.
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP MF_00361
Cofactor	Divalent metal ions By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 295	295	Probable inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361		PRO_1000120830

Sequences

	Sequence		Length	Mass (Da)
	B7J4J4-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 72FC392688828196	FASTA	295	31,754
	10 20 30 40 50 60 MTQPFQRVLL VSKYHDPSVL PGLRQLRDFL LARDMPTFLE SQSAADIGDS LGLPLLSFAE 70 80 90 100 110 120 ADAGSDLVIA LGGDGTLLGT ARQTAQSGIP ILGINQGRLG FLADLSIHQI SEALPPILEG 130 140 150 160 170 180 HYQQDLRSIL HAELWRSEER VHTGLAVNEV FIHKGGGESM IELQVQMDGR FVYTQRADGL 190 200 210 220 230 240 IIATPTGSTA YAMSAGGPIL TPTLAALLLV LICPHTLTAR PLAVADSVEI VARLTASRQS 250 260 270 280 290 AALSLDSHCS VPLEIGDEIV IRRASCAARF IHPEEENFFQ ILRGKLHWAD SPGTD

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001219 Genomic DNA. Translation: ACK78828.1.
RefSeq	YP_002424947.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7134667.
GenomeReviews	Gene locus AFE_0451 in contig CP001219_GR.
KEGG	afr:AFE_0451.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00361. [Tree]
InterPro	IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. ATP_NADK. [Graphical view]
Gene3D	G3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit.
PANTHER	PTHR20275. ATP_NADK. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPNK_ACIF2

Accession

Primary (citable) accession number: B7J4J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	October 13, 2009
This is version 9 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)