Skip Header

Contribute Send feedback
Read comments (?) or add your own

B7J4C0 (ISPD_ACIF2) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT
Gene names
Name:ispD
Ordered Locus Names:AFE_0370
OrganismAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]
Taxonomic identifier243159 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length237 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108

Sequence similarities

Belongs to the IspD family.

Ontologies

Keywords
   Biological processIsoprene biosynthesis
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processisoprenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2372372-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
PRO_1000117435

Sites

Site161Transition state stabilizer By similarity
Site231Transition state stabilizer By similarity
Site1601Positions MEP for the nucleophilic attack By similarity
Site2161Positions MEP for the nucleophilic attack By similarity

Sequences

Sequence LengthMass (Da)Tools
B7J4C0 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 21509CB14B1B633C

FASTA23725,106
        10         20         30         40         50         60 
MERVWVVIPA GGRGQRFGAA QAKQYVLLRD RPVIAHTLAA FLGEPRIAGI QLVLPGEDIA 

        70         80         90        100        110        120 
TGAWRELLGP MPAPLLPPVV GGGLRADSVR LGLEALLRQG AVPSDWVLVH DAARPCLRRE 

       130        140        150        160        170        180 
DLLRLLESLA NAPQGALLAV PVADTLKRGE DGCSSGTVDR EGLWRALTPQ AFPLGALLAA 

       190        200        210        220        230 
LEAARAGNRQ ITDEASAMEA QGWRPRLIPG HGDNIKVTLS DDLMLAAAIL AARSEEG

« Hide

References

[1]"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications."
Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S.
BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001219 Genomic DNA. Translation: ACK80882.1.
RefSeqYP_002424873.1. NC_011761.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB7J4C0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID7134540.
GenomeReviewsGene locus AFE_0370 in contig CP001219_GR.
KEGGafr:AFE_0370.
PATRIC20652075. VBIAciFer29821_0358.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG672839.
OMAFPQGAIL.
ProtClustDBCLSK2407431.

Family and domain databases

HAMAPMF_00108. IspD.
[Tree]
InterProIPR001228. ISPD_synthase.
IPR018294. ISPD_synthase_CS.
[Graphical view]
KOK00991.
PfamPF01128. IspD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00453. IspD. 1 hit.
PROSITEPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPD_ACIF2
AccessionPrimary (citable) accession number: B7J4C0
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: February 10, 2009
Last modified: January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents