Reviewed, UniProtKB/Swiss-Prot B7J439 (GLYA_ACIF2)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase
      Short name=Serine methylase
      Short name=SHMT
    EC=2.1.2.1

Gene names

Name:	glyA
Ordered Locus Names:	AFE_0295

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Serine hydroxymethyltransferase HAMAP MF_00051		PRO_0000369894
Amino acid modifications
	Modified residue	230	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B7J439-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 9D7CFAB5AE075822	FASTA	414	44,498
	10 20 30 40 50 60 MFSKTLTIAD FDPVLWDAMR KEARRQEDHV ELIASENYAS PMVMAAQGSV LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEY VDIAEQLAMD RALELFGAEH ANVQAHSGSQ ANQAVYLSVL QPGDKIMGMS 130 140 150 160 170 180 LAHGGHLTHG AKVNVSGKLF QVAAYGVRAE DGRIDYDAMA EQAERERPKM IVAGASAYSR 190 200 210 220 230 240 VIDFARIGEI ARSIGAYLLV DMAHIAGLVA TGLHPSPVPH ADFVTTTTHK TLRGPRGGLI 250 260 270 280 290 300 LCREQYAKKV NSLIFPGLQG GPLMHVIAAK AVAFREALQP EFKSYQQQVI HNAQTLSKVL 310 320 330 340 350 360 AGRGYGAVSG GTDNHLFLLN LGEKVTGKEA EEALGQANIT VNKNAVPFDI RPPAVTSGIR 370 380 390 400 410 IGTPAATTRG FGEAEMHRLG NGIADVLDAS SDAAVIERVR ADMKALCHQF PVYG

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001219 Genomic DNA. Translation: ACK78912.1.
RefSeq	YP_002424802.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7136603.
GenomeReviews	Gene locus AFE_0295 in contig CP001219_GR.
KEGG	afr:AFE_0295.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA_ACIF2

Accession

Primary (citable) accession number: B7J439

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	September 22, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)