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B7J415

- TRMB_ACIF2

UniProt

B7J415 - TRMB_ACIF2

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, AFE_0271
Organism
Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei58 – 581S-adenosyl-L-methionine By similarity
Binding sitei83 – 831S-adenosyl-L-methionine By similarity
Binding sitei110 – 1101S-adenosyl-L-methionine By similarity
Active sitei132 – 1321 By similarity
Binding sitei132 – 1321S-adenosyl-L-methionine By similarity
Binding sitei136 – 1361Substrate By similarity
Binding sitei168 – 1681Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciAFER243159:GH3S-271-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:AFE_0271
    OrganismiAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
    Taxonomic identifieri243159 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus
    ProteomesiUP000001362: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 227227tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_1000213437Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi243159.AFE_0271.

    Structurei

    3D structure databases

    ProteinModelPortaliB7J415.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni205 – 2084Substrate binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiTSATNDY.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B7J415-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEALKTSHR AIRSFVLRQG RITAAQTRAV AEYLPARQID AHAEWRDPWN    50
    NQRPLMLEIG FGNGEHLAAI AAQRPTWGCI GIEVHTPGVG SLLLQLVEAG 100
    TDNVRIVHDD AVTWLKTLPD AILRCIIIQF PDPWPKKRQQ KRRLIQPDFA 150
    AVLCRLLQPG GELQLATDWA DYAGQMLTVL NATPGLQNAD ADNGYVFRPD 200
    NRILTRFERR GQRLGHAVYD LCYRRIP 227
    Length:227
    Mass (Da):25,673
    Last modified:February 10, 2009 - v1
    Checksum:i15DC93572873E562
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001219 Genomic DNA. Translation: ACK78559.1.
    RefSeqiWP_012606533.1. NC_011761.1.
    YP_002424778.1. NC_011761.1.

    Genome annotation databases

    EnsemblBacteriaiACK78559; ACK78559; AFE_0271.
    GeneIDi7134929.
    KEGGiafr:AFE_0271.
    PATRICi20651879. VBIAciFer29821_0264.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001219 Genomic DNA. Translation: ACK78559.1 .
    RefSeqi WP_012606533.1. NC_011761.1.
    YP_002424778.1. NC_011761.1.

    3D structure databases

    ProteinModelPortali B7J415.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 243159.AFE_0271.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACK78559 ; ACK78559 ; AFE_0271 .
    GeneIDi 7134929.
    KEGGi afr:AFE_0271.
    PATRICi 20651879. VBIAciFer29821_0264.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi TSATNDY.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci AFER243159:GH3S-271-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications."
      Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S.
      BMC Genomics 9:597-597(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

    Entry informationi

    Entry nameiTRMB_ACIF2
    AccessioniPrimary (citable) accession number: B7J415
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: February 10, 2009
    Last modified: September 3, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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