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B7J415 (TRMB_ACIF2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:AFE_0271
OrganismAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]
Taxonomic identifier243159 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 227227tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000213437

Regions

Region205 – 2084Substrate binding By similarity

Sites

Active site1321 By similarity
Binding site581S-adenosyl-L-methionine By similarity
Binding site831S-adenosyl-L-methionine By similarity
Binding site1101S-adenosyl-L-methionine By similarity
Binding site1321S-adenosyl-L-methionine By similarity
Binding site1361Substrate By similarity
Binding site1681Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B7J415 [UniParc].

Last modified February 10, 2009. Version 1.
Checksum: 15DC93572873E562

FASTA22725,673
        10         20         30         40         50         60 
MEEALKTSHR AIRSFVLRQG RITAAQTRAV AEYLPARQID AHAEWRDPWN NQRPLMLEIG 

        70         80         90        100        110        120 
FGNGEHLAAI AAQRPTWGCI GIEVHTPGVG SLLLQLVEAG TDNVRIVHDD AVTWLKTLPD 

       130        140        150        160        170        180 
AILRCIIIQF PDPWPKKRQQ KRRLIQPDFA AVLCRLLQPG GELQLATDWA DYAGQMLTVL 

       190        200        210        220 
NATPGLQNAD ADNGYVFRPD NRILTRFERR GQRLGHAVYD LCYRRIP 

« Hide

References

[1]"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications."
Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S.
BMC Genomics 9:597-597(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001219 Genomic DNA. Translation: ACK78559.1.
RefSeqYP_002424778.1. NC_011761.1.

3D structure databases

ProteinModelPortalB7J415.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243159.AFE_0271.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACK78559; ACK78559; AFE_0271.
GeneID7134929.
KEGGafr:AFE_0271.
PATRIC20651879. VBIAciFer29821_0264.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMATSATNDY.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycAFER243159:GH3S-271-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_ACIF2
AccessionPrimary (citable) accession number: B7J415
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 10, 2009
Last modified: June 11, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways