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B7J415

- TRMB_ACIF2

UniProt

B7J415 - TRMB_ACIF2

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei58 – 581S-adenosyl-L-methionineUniRule annotation
Binding sitei83 – 831S-adenosyl-L-methionineUniRule annotation
Binding sitei110 – 1101S-adenosyl-L-methionineUniRule annotation
Active sitei132 – 1321By similarity
Binding sitei132 – 1321S-adenosyl-L-methionineUniRule annotation
Binding sitei136 – 1361SubstrateUniRule annotation
Binding sitei168 – 1681SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAFER243159:GH3S-271-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:AFE_0271
OrganismiAcidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270))
Taxonomic identifieri243159 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAcidithiobacillalesAcidithiobacillaceaeAcidithiobacillus
ProteomesiUP000001362: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227tRNA (guanine-N(7)-)-methyltransferasePRO_1000213437Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243159.AFE_0271.

Structurei

3D structure databases

ProteinModelPortaliB7J415.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni205 – 2084Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiTSATNDY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B7J415-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEEALKTSHR AIRSFVLRQG RITAAQTRAV AEYLPARQID AHAEWRDPWN
60 70 80 90 100
NQRPLMLEIG FGNGEHLAAI AAQRPTWGCI GIEVHTPGVG SLLLQLVEAG
110 120 130 140 150
TDNVRIVHDD AVTWLKTLPD AILRCIIIQF PDPWPKKRQQ KRRLIQPDFA
160 170 180 190 200
AVLCRLLQPG GELQLATDWA DYAGQMLTVL NATPGLQNAD ADNGYVFRPD
210 220
NRILTRFERR GQRLGHAVYD LCYRRIP
Length:227
Mass (Da):25,673
Last modified:February 10, 2009 - v1
Checksum:i15DC93572873E562
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001219 Genomic DNA. Translation: ACK78559.1.
RefSeqiYP_002424778.1. NC_011761.1.

Genome annotation databases

EnsemblBacteriaiACK78559; ACK78559; AFE_0271.
GeneIDi7134929.
KEGGiafr:AFE_0271.
PATRICi20651879. VBIAciFer29821_0264.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001219 Genomic DNA. Translation: ACK78559.1 .
RefSeqi YP_002424778.1. NC_011761.1.

3D structure databases

ProteinModelPortali B7J415.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243159.AFE_0271.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACK78559 ; ACK78559 ; AFE_0271 .
GeneIDi 7134929.
KEGGi afr:AFE_0271.
PATRICi 20651879. VBIAciFer29821_0264.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OMAi TSATNDY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci AFER243159:GH3S-271-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications."
    Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S.
    BMC Genomics 9:597-597(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23270 / DSM 14882 / NCIB 8455.

Entry informationi

Entry nameiTRMB_ACIF2
AccessioniPrimary (citable) accession number: B7J415
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: February 10, 2009
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3