Reviewed, UniProtKB/Swiss-Prot B7J3V1 (MURC_ACIF2)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramate--L-alanine ligase
    EC=6.3.2.8
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	AFE_0205

Organism

Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) (Ferrobacillus ferrooxidans (strain ATCC 23270)) [Complete proteome] [HAMAP]

Taxonomic identifier

243159 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Acidithiobacillales › Acidithiobacillaceae › Acidithiobacillus

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000116613
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B7J3V1-1 [UniParc]. Last modified February 10, 2009. Version 1. Checksum: 6B3097FEF9CFBA03	FASTA	464	49,171
	10 20 30 40 50 60 MRNWVRQIHM VGIGGSGMRG IAEVLLNLGY AVSGSDLRPG NSTLRLTDLG ARIFSGHEAA 70 80 90 100 110 120 NVRGADVVVI SSAIPESNPE VQAARELRIP VIRRAEMLAE LMRFKQGIAI AGTHGKTTTT 130 140 150 160 170 180 SLVASILGAG GLDPTFVIGG RLKSAGTHAA LGSGEYLVAE ADESDASFLY LSPVMAVVTN 190 200 210 220 230 240 IDADHMETYG GSLDNLRGAF LQFLQRLPFY GLAVLCTDEA VVAGLIPELR TPVLRYGFGA 250 260 270 280 290 300 DADLQARDVT VQGIGSRFAV WRRVDSDYVH WLDVELGIPG RHNVLNALAA IGIASKVGIP 310 320 330 340 350 360 ESTIATALAD FRGVGRRFEL VGTFDGITVV DDYGHHPREI AATIAAARSV WPERPLVVAF 370 380 390 400 410 420 QPHRYSRTQA LFADFVSSLA AADRVILTDI YSAGEKALPG VTGRALAEAM AAQGMSVRFL 430 440 450 460 PDLLTAPQQI RAELPAGAVL LTLGAGSIAS LAAQWPQVFG EDPS

References

[1]

"Acidithiobacillus ferrooxidans metabolism: from genome sequence to industrial applications." Valdes J., Pedroso I., Quatrini R., Dodson R.J., Tettelin H., Blake R. II, Eisen J.A., Holmes D.S. BMC Genomics 9:597-597(2008) [PubMed: 19077236] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001219 Genomic DNA. Translation: ACK80005.1.
RefSeq	YP_002424714.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7135338.
GenomeReviews	Gene locus AFE_0205 in contig CP001219_GR.
KEGG	afr:AFE_0205.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00046. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01082. murC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_ACIF2

Accession

Primary (citable) accession number: B7J3V1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 10, 2009
Last modified:	June 16, 2009
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)